Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow FMOD rightarrow Mouse FMOD  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • aldehyde dehydrogenase 5 family, member A1 (ALDH5A1) ELISA Kit
 • Pfu DNA Polymerase (PF0212) Recombinant Protein
 • Complement Component 4d (C4d) ELISA Kit
 • Glycated Hemoglobin A1c (HbA1c) ELISA Kit
 • Insulin Like Growth Factor-1 (IGF1) Recombinant Protein
 • SLC6A15 Antibody
 • APBA2 Antibody
 • CD104 (beta 4 Integrin) Antibody
 • CD122 (IL2RB) Blocking Peptide
 • ADAM metallopeptidase with thrombospondin type 1 motif, 12 (... ELISA Kit
 • Norovirus Antibody
 • ABCC8 Antibody
 • A-L-Iduronidase (IDUA) ELISA Kit
 • Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

FMOD elisa kit :: Mouse Fibromodulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733452
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Fibromodulin (FMOD), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Fibromodulin ELISA Kit

 Product Gene Name   

FMOD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600245
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of FMOD. No significant cross-reactivity or interference between FMOD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FMOD and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    1.0-25ng/mL
 Sensitivity    0.1ng/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FMOD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FMOD purchase    MBS733452 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fibromodulin (FMOD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1.0-25ng/mL in biological research samples containing FMOD, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS733452 kit is based on FMOD antibody-FMOD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FMOD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FMOD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for FMOD elisa kit

   Intended Uses: This FMOD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FMOD. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FMOD ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FMOD. Standards or samples are then added to the microtiter plate wells and FMOD if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FMOD present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FMOD are added to each well to "sandwich" the FMOD immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FMOD and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FMOD concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for FMOD elisa kit    Cardiovascular
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of FMOD elisa kit    FMOD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS733452. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for FMOD. It may not necessarily be applicable to this product.
 NCBI GI #    71040111
 NCBI GeneID    2331
 NCBI Accession #    NP_002014.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002023.4 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q15331; Q8IV47 [Other Products]
 UniProt Related Accession #    Q06828 [Other Products]
 Molecular Weight    43,179 Da
Table BarTOPTable Bar
 NCBI Official Full Name    fibromodulin
 NCBI Official Synonym Full Names    fibromodulin
 NCBI Official Symbol    FMOD [Similar Products]
 NCBI Official Synonym Symbols   
FM; SLRR2E
[Similar Products]
 NCBI Protein Information    fibromodulin; KSPG fibromodulin; collagen-binding 59 kDa protein; keratan sulfate proteoglycan fibromodulin
 UniProt Protein Name    Fibromodulin
 UniProt Synonym Protein Names   
Collagen-binding 59 kDa protein; Keratan sulfate proteoglycan fibromodulin; KSPG fibromodulin
 Protein Family    Fibromodulin
 UniProt Gene Name    FMOD [Similar Products]
 UniProt Synonym Gene Names    FM; SLRR2E; FM; KSPG fibromodulin [Similar Products]
 UniProt Entry Name    FMOD_HUMAN
Section Bar
 NCBI Summary for FMOD    Fibromodulin belongs to the family of small interstitial proteoglycans. The encoded protein possesses a central region containing leucine-rich repeats with 4 keratan sulfate chains, flanked by terminal domains containing disulphide bonds. Owing to the interaction with type I and type II collagen fibrils and in vitro inhibition of fibrillogenesis, the encoded protein may play a role in the assembly of extracellular matrix. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. Sequence variations in this gene may be associated with the pathogenesis of high myopia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Section Bar
 UniProt Comments for FMOD    FMOD: Affects the rate of fibrils formation. May have a primary role in collagen fibrillogenesis. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; extracellular region

Biological Process: transforming growth factor beta receptor complex assembly; keratan sulfate metabolic process; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; carbohydrate metabolic process; pathogenesis; keratan sulfate catabolic process
Table BarTOPTable Bar
 Research Articles on FMOD    1. Melanocyte-secreted factor FMOD is a key regulator of angiogenesis.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with FMOD elisa kitOrgans/Tissues associated with FMOD elisa kit
 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 Keratan Sulfate Biosynthesis Pathway antibodies  Keratan Sulfate Biosynthesis Pathway Diagram
 Keratan Sulfate Degradation Pathway antibodies  Keratan Sulfate Degradation Pathway Diagram
 Keratan Sulfate/keratin Metabolism Pathway antibodies  Keratan Sulfate/keratin Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >19 publications with FMOD and Bone
 Connective Tissue Antibodies  >13 publications with FMOD and Connective Tissue
 Blood Antibodies  >11 publications with FMOD and Blood
 Eye Antibodies  >6 publications with FMOD and Eye
 Vascular Antibodies  >6 publications with FMOD and Vascular
 Skin Antibodies  >5 publications with FMOD and Skin
 Brain Antibodies  >4 publications with FMOD and Brain
 Embryonic Tissue Antibodies  >4 publications with FMOD and Embryonic Tissue
 Kidney Antibodies  >3 publications with FMOD and Kidney
 Mouth Antibodies  >3 publications with FMOD and Mouth
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions