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FLG elisa kit :: Mouse Filaggrin (FLG) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2024386 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Filaggrin (FLG), ELISA Kit

★Popular Item★
 Also Known As   

Filaggrin (FLG) ELISA Kit

 Product Synonym Names    Profilaggrin
 Product Gene Name   

FLG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    135940
 3D Structure    ModBase 3D Structure for P20930
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Filaggrin (FLG).
No significant cross-reactivity or interference between Filaggrin (FLG) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.114ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Filaggrin (FLG) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Filaggrin (FLG) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of FLG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for FLG purchase    MBS2024386 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Filaggrin (FLG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing FLG, with an estimated sensitivity of < 0.114ng/mL. The ELISA analytical biochemical technique of the MBS2024386 kit is based on FLG antibody-FLG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FLG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FLG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for FLG elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filaggrin (FLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filaggrin (FLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filaggrin (FLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filaggrin (FLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for FLG elisa kit    Metabolic pathway
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 Typical Testing Data/Standard Curve (for reference only) of FLG elisa kit    FLG elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2024386. Click to request current manual
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NCBI/Uniprot data below describe general gene information for FLG. It may not necessarily be applicable to this product.
 NCBI GI #    60097902
 NCBI GeneID    2312
 NCBI Accession #    NP_002007.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002016.1 [Other Products]
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 UniProt Primary Accession #    P20930 [Other Products]
 UniProt Secondary Accession #    Q01720; Q5T583; Q9UC71 [Other Products]
 UniProt Related Accession #    P20930 [Other Products]
 Molecular Weight    435,170 Da
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 NCBI Official Full Name    filaggrin
 NCBI Official Synonym Full Names    filaggrin
 NCBI Official Symbol    FLG [Similar Products]
 NCBI Official Synonym Symbols   
ATOD2
[Similar Products]
 NCBI Protein Information    filaggrin
 UniProt Protein Name    Filaggrin
 Protein Family    Filaggrin
 UniProt Gene Name    FLG [Similar Products]
 UniProt Entry Name    FILA_HUMAN
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 NCBI Summary for FLG    The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
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 UniProt Comments for FLG    FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus

Molecular Function: protein binding; calcium ion binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2
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 Research Articles on FLG    1. IL-33 increases the skin barrier permeability to allergens by downregulating the expression of filaggrin in atopic dermatitis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with FLG elisa kitPathways associated with FLG elisa kit
 Reference Product  PubMed Publications
 FGFR1 elisa kit  >38 publications with FLG and FGFR1
 LOR elisa kit  >12 publications with FLG and LOR
 SPINK5 elisa kit  >8 publications with FLG and SPINK5
 IVL elisa kit  >7 publications with FLG and IVL
 EGF elisa kit  >5 publications with FLG and EGF
 KRT1 elisa kit  >1 publications with FLG and KRT1
 Products by Pathway  Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
Diseases associated with FLG elisa kitOrgans/Tissues associated with FLG elisa kit
 Disease Name  Pubmed Publications
 Dermatitis, Atopic Antibodies  >192 publications with FLG and Dermatitis, Atopic
 Dermatitis, Atopic, 1 Antibodies  >112 publications with FLG and Dermatitis, Atopic, 1
 Dermatitis, Atopic, 2 Antibodies  >95 publications with FLG and Dermatitis, Atopic, 2
 Eczema Antibodies  >77 publications with FLG and Eczema
 Ichthyosis Antibodies  >69 publications with FLG and Ichthyosis
 Ichthyosis Vulgaris Antibodies  >64 publications with FLG and Ichthyosis Vulgaris
 Inflammation Antibodies  >37 publications with FLG and Inflammation
 Lung Diseases Antibodies  >33 publications with FLG and Lung Diseases
 Psoriasis Antibodies  >15 publications with FLG and Psoriasis
 Necrosis Antibodies  >12 publications with FLG and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >207 publications with FLG and Skin
 Brain Antibodies  >31 publications with FLG and Brain
 Uterus Antibodies  >3 publications with FLG and Uterus
 Mouth Antibodies  >2 publications with FLG and Mouth
 Testis Antibodies  >2 publications with FLG and Testis
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