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GSDMD elisa kit :: Mouse Gasdermin D ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2031861 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Gasdermin D (GSDMD), ELISA Kit

★Popular Item★
 Also Known As   

Gasdermin D (GSDMD) ELISA Kit

 Product Synonym Names    GSDMDC1; DF5L; Gasdermin Domain Containing 1
 Product Gene Name   

GSDMD elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Gasdermin D (GSDMD).
No significant cross-reactivity or interference between Gasdermin D (GSDMD) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.063ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Gasdermin D (GSDMD) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Gasdermin D (GSDMD) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GSDMD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GSDMD purchase    MBS2031861 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gasdermin D (GSDMD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing GSDMD, with an estimated sensitivity of < 0.063ng/mL. The ELISA analytical biochemical technique of the MBS2031861 kit is based on GSDMD antibody-GSDMD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GSDMD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GSDMD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GSDMD elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gasdermin D (GSDMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gasdermin D (GSDMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gasdermin D (GSDMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gasdermin D (GSDMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for GSDMD elisa kit    Signal transduction
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 Typical Testing Data/Standard Curve (for reference only) of GSDMD elisa kit    GSDMD elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2031861. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GSDMD. It may not necessarily be applicable to this product.
 NCBI GI #    261878494
 NCBI GeneID    79792
 NCBI Accession #    NP_001159709.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001166237.1 [Other Products]
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 UniProt Secondary Accession #    Q96Q98; D3DWJ9 [Other Products]
 UniProt Related Accession #    P57764 [Other Products]
 Molecular Weight    52,801 Da
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 NCBI Official Full Name    gasdermin-D
 NCBI Official Synonym Full Names    gasdermin D
 NCBI Official Symbol    GSDMD [Similar Products]
 NCBI Official Synonym Symbols   
DF5L; DFNA5L; FKSG10; GSDMDC1
[Similar Products]
 NCBI Protein Information    gasdermin-D
 UniProt Protein Name    Gasdermin-D
 UniProt Synonym Protein Names   
Gasdermin domain-containing protein 1
 Protein Family    Gasdermin
 UniProt Gene Name    GSDMD [Similar Products]
 UniProt Synonym Gene Names    DFNA5L; GSDMDC1 [Similar Products]
 UniProt Entry Name    GSDMD_HUMAN
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 NCBI Summary for GSDMD    Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
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 UniProt Comments for GSDMD    FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm

Molecular Function: protein binding

Biological Process: cellular response to extracellular stimulus
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 Research Articles on GSDMD    1. Study investigated the expression pattern of the GSDM family genes in the upper gastrointestinal epithelium and cancers.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GSDMD elisa kitDiseases associated with GSDMD elisa kit
 Reference Product  PubMed Publications
 TIGD5 elisa kit  >1 publications with GSDMD and TIGD5
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with GSDMD and Necrosis
Organs/Tissues associated with GSDMD elisa kit
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >2 publications with GSDMD and Skin
 Bone Marrow Antibodies  >1 publications with GSDMD and Bone Marrow
 Esophagus Antibodies  >1 publications with GSDMD and Esophagus
 Bone Antibodies  >1 publications with GSDMD and Bone
 Stomach Antibodies  >1 publications with GSDMD and Stomach
 Prostate Antibodies  >1 publications with GSDMD and Prostate
 Salivary Gland Antibodies  >1 publications with GSDMD and Salivary Gland
 Intestine Antibodies  >1 publications with GSDMD and Intestine
 Placenta Antibodies  >1 publications with GSDMD and Placenta
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