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| Product Name
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Ghrelin (GHRL), ELISA Kit ★Popular Item★
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| Also Known As
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Mouse Ghrelin (GHRL) ELISA Kit
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Sample Manual Insert
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| Request for Current Manual Insert
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| OMIM
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605353
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| Species Reactivity
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Mouse
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| Specificity
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No cross-reaction with other factors.
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| Samples
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Mouse serum, plasma or cell culture supernatant and organizations
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| Assay Type
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Quantitative Sandwich
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| Detection Range
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10 ng/ml-0.156 ng/ml
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| Sensitivity
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0.05 ng/ml
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| Intra-assay Precision
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<= 8%
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| Inter-assay Precision
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<= 12%
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| Preparation and Storage
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Store all reagents at 2-8 degree C.
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| Product Note
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Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
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| Other Notes
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Small volumes of GHRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Searchable Terms for GHRL purchase
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MBS2601787 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin (GHRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing GHRL, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS2601787 kit is based on GHRL antibody-GHRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or cell culture supernatant and organizations. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GHRL elisa kit
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Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GHRL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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| Typical Testing Data/Standard Curve (for reference only) of GHRL elisa kit
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| Sample Manual Insert of MBS2601787. Click to request current manual
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| NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product. |
| NCBI GI #
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6691572
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| NCBI GeneID
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51738
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| NCBI Accession #
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BAA89371.1
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[Other Products]
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| UniProt Secondary Accession #
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Q86YP8; Q8TAT9; Q9H3R3; A8CF34; A8CF38; A8CF42; A8DN29; A8DN30
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[Other Products]
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| UniProt Related Accession #
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Q9UBU3
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[Other Products]
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| Molecular Weight
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9,972 Da
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| NCBI Official Full Name
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ghrelin
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| NCBI Official Synonym Full Names
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ghrelin/obestatin prepropeptide
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| NCBI Official Symbol
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GHRL
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[Similar Products]
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| NCBI Official Synonym Symbols
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MTLRP
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[Similar Products]
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| NCBI Protein Information
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appetite-regulating hormone; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide; prepro-appetite regulatory hormone; ghrelin, growth hormone secretagogue receptor ligand
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| UniProt Protein Name
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Appetite-regulating hormone
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| UniProt Synonym Protein Names
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Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Cleaved into the following 3 chains:Ghrelin-27; Ghrelin-28; Ghrelin; Obestatin
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| Protein Family
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Ghrelin
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| UniProt Gene Name
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GHRL
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[Similar Products]
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| UniProt Synonym Gene Names
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MTLRP; UNQ524/PRO1066; Ghrelin
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[Similar Products]
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| UniProt Entry Name
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GHRL_HUMAN
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| NCBI Summary for GHRL
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This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]
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| UniProt Comments for GHRL
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ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach
Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cell development/differentiation; Apoptosis
Chromosomal Location of Human Ortholog: 3p26-p25
Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region
Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity
Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis
Disease: Obesity
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| Research Articles on GHRL
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1. Association of the GHRL Leu72Met polymorphism and resistance to diabetic nephropathy in Chinese type 2 diabetes mellitus patients.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with GHRL elisa kit | Pathways associated with GHRL elisa kit |
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| Diseases associated with GHRL elisa kit | Organs/Tissues associated with GHRL elisa kit |
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ELISA Kit 
