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GHRL elisa kit :: Mouse Ghrelin (GHRL) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2601787
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ghrelin (GHRL), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Ghrelin (GHRL) ELISA Kit

 Product Gene Name   

GHRL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605353
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Mouse serum, plasma or cell culture supernatant and organizations
 Assay Type    Quantitative Sandwich
 Detection Range    10 ng/ml-0.156 ng/ml
 Sensitivity    0.05 ng/ml
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GHRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GHRL purchase    MBS2601787 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin (GHRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156 ng/ml in biological research samples containing GHRL, with an estimated sensitivity of 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS2601787 kit is based on GHRL antibody-GHRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or cell culture supernatant and organizations. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GHRL elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse GHRL monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of GHRL elisa kit    GHRL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2601787. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
 NCBI GI #    6691572
 NCBI GeneID    51738
 NCBI Accession #    BAA89371.1 [Other Products]
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 UniProt Secondary Accession #    Q86YP8; Q8TAT9; Q9H3R3; A8CF34; A8CF38; A8CF42; A8DN29; A8DN30 [Other Products]
 UniProt Related Accession #    Q9UBU3 [Other Products]
 Molecular Weight    9,972 Da
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 NCBI Official Full Name    ghrelin
 NCBI Official Synonym Full Names    ghrelin/obestatin prepropeptide
 NCBI Official Symbol    GHRL [Similar Products]
 NCBI Official Synonym Symbols   
MTLRP
[Similar Products]
 NCBI Protein Information    appetite-regulating hormone; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide; prepro-appetite regulatory hormone; ghrelin, growth hormone secretagogue receptor ligand
 UniProt Protein Name    Appetite-regulating hormone
 UniProt Synonym Protein Names   
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Cleaved into the following 3 chains:Ghrelin-27; Ghrelin-28; Ghrelin; Obestatin
 Protein Family    Ghrelin
 UniProt Gene Name    GHRL [Similar Products]
 UniProt Synonym Gene Names    MTLRP; UNQ524/PRO1066; Ghrelin [Similar Products]
 UniProt Entry Name    GHRL_HUMAN
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 NCBI Summary for GHRL    This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]
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 UniProt Comments for GHRL    ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cell development/differentiation; Apoptosis

Chromosomal Location of Human Ortholog: 3p26-p25

Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity

Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis

Disease: Obesity
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 Research Articles on GHRL    1. Association of the GHRL Leu72Met polymorphism and resistance to diabetic nephropathy in Chinese type 2 diabetes mellitus patients.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GHRL elisa kitPathways associated with GHRL elisa kit
 Reference Product  PubMed Publications
 GHSR elisa kit  >24 publications with GHRL and GHSR
 LEP elisa kit  >11 publications with GHRL and LEP
 NPY elisa kit  >11 publications with GHRL and NPY
 CCK elisa kit  >5 publications with GHRL and CCK
 GHRH elisa kit  >3 publications with GHRL and GHRH
 AGRP elisa kit  >3 publications with GHRL and AGRP
 GCG elisa kit  >2 publications with GHRL and GCG
 IGF1 elisa kit  >1 publications with GHRL and IGF1
 GPR39 elisa kit  >1 publications with GHRL and GPR39
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with GHRL elisa kitOrgans/Tissues associated with GHRL elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >52 publications with GHRL and Obesity
 Insulin Resistance Antibodies  >18 publications with GHRL and Insulin Resistance
 Nervous System Diseases Antibodies  >13 publications with GHRL and Nervous System Diseases
 Weight Gain Antibodies  >12 publications with GHRL and Weight Gain
 Inflammation Antibodies  >9 publications with GHRL and Inflammation
 Cachexia Antibodies  >6 publications with GHRL and Cachexia
 Necrosis Antibodies  >5 publications with GHRL and Necrosis
 Heart Diseases Antibodies  >5 publications with GHRL and Heart Diseases
 Breast Neoplasms Antibodies  >5 publications with GHRL and Breast Neoplasms
 Hypertension Antibodies  >4 publications with GHRL and Hypertension
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >75 publications with GHRL and Blood
 Stomach Antibodies  >27 publications with GHRL and Stomach
 Brain Antibodies  >23 publications with GHRL and Brain
 Connective Tissue Antibodies  >10 publications with GHRL and Connective Tissue
 Intestine Antibodies  >4 publications with GHRL and Intestine
 Prostate Antibodies  >1 publications with GHRL and Prostate
 Embryonic Tissue Antibodies  >1 publications with GHRL and Embryonic Tissue
 Lung Antibodies  >1 publications with GHRL and Lung
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