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Gcg elisa kit :: Mouse Glucagon ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886862
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $420 +1 FREE 8GB USB
96-Strip-Wells  /  $560 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,445 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,520 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glucagon (Gcg), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Glucagon ELISA Kit

 Product Synonym Names    Glucagon; Gcg
 Product Gene Name   

Gcg elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886862 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P55095
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    15.6-1000 pg/mL
 Sensitivity    8.04 pg/mL
 Intra-Assay CV    <=4.3%
 Inter-Assay CV    <=6.4%
 Recovery    102%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Gcg elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Gcg purchase    MBS2886862 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucagon (Gcg) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000 pg/mL in biological research samples containing Gcg, with an estimated sensitivity of 8.04 pg/mL. The ELISA analytical biochemical technique of the MBS2886862 kit is based on Gcg antibody-Gcg antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gcg antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gcg. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Gcg elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Standard Curve/Testing Data of Gcg elisa kit    Gcg elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2886862. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Gcg. It may not necessarily be applicable to this product.
 NCBI GI #    33468853
 NCBI GeneID    14526
 NCBI Accession #    NP_032126.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008100.4 [Other Products]
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 UniProt Primary Accession #    P55095 [Other Products]
 UniProt Secondary Accession #    Q3UFE9 [Other Products]
 UniProt Related Accession #    P55095 [Other Products]
 Molecular Weight    20,906 Da
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 NCBI Official Full Name    glucagon preproprotein
 NCBI Official Synonym Full Names    glucagon
 NCBI Official Symbol    Gcg [Similar Products]
 NCBI Official Synonym Symbols   
Glu; PPG; GLP-1
[Similar Products]
 NCBI Protein Information    glucagon
 UniProt Protein Name    Glucagon
 Protein Family    Glucagon
 UniProt Gene Name    Gcg [Similar Products]
 UniProt Synonym Gene Names    GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2 [Similar Products]
 UniProt Entry Name    GLUC_MOUSE
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 NCBI Summary for Gcg    This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015]
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 UniProt Comments for Gcg    GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: glucagon receptor binding; hormone activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; negative regulation of appetite; positive regulation of cAMP biosynthetic process; positive regulation of histone H3-K4 methylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein kinase activity; regulation of insulin secretion; response to starvation
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 Research Articles on Gcg    1. Deoxynivalenol induced cholecystokinin and glucagon-like peptide 1 release by enteroendocrine cells in mediated by casR/TRPA1 signaling.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Gcg elisa kitPathways associated with Gcg elisa kit
 Reference Product  PubMed Publications
 Glp1r elisa kit  >10 publications with Gcg and Glp1r
 Tcf7l2 elisa kit  >6 publications with Gcg and Tcf7l2
 Gcgr elisa kit  >5 publications with Gcg and Gcgr
 Glp2r elisa kit  >4 publications with Gcg and Glp2r
 Pax6 elisa kit  >4 publications with Gcg and Pax6
 Ghrl elisa kit  >3 publications with Gcg and Ghrl
 Gipr elisa kit  >2 publications with Gcg and Gipr
 Ins2 elisa kit  >1 publications with Gcg and Ins2
 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Glucagon Signaling In Metabolic Regulation Pathway antibodies  Glucagon Signaling In Metabolic Regulation Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram
 Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway antibodies  Glucagon-like Peptide-1 (GLP1) Regulates Insulin Secretion Pathway Diagram
Diseases associated with Gcg elisa kitOrgans/Tissues associated with Gcg elisa kit
 Disease Name  Pubmed Publications
 Muscular Diseases Antibodies  >73 publications with Gcg and Muscular Diseases
 Diabetes Mellitus, Type 2 Antibodies  >28 publications with Gcg and Diabetes Mellitus, Type 2
 Obesity Antibodies  >21 publications with Gcg and Obesity
 Vascular Diseases Antibodies  >15 publications with Gcg and Vascular Diseases
 Insulin Resistance Antibodies  >13 publications with Gcg and Insulin Resistance
 Hyperglycemia Antibodies  >12 publications with Gcg and Hyperglycemia
 Kidney Diseases Antibodies  >11 publications with Gcg and Kidney Diseases
 Hyperinsulinism Antibodies  >10 publications with Gcg and Hyperinsulinism
 Liver Diseases Antibodies  >9 publications with Gcg and Liver Diseases
 Pain Antibodies  >9 publications with Gcg and Pain
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >48 publications with Gcg and Pancreas
 Brain Antibodies  >47 publications with Gcg and Brain
 Intestine Antibodies  >21 publications with Gcg and Intestine
 Eye Antibodies  >15 publications with Gcg and Eye
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