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GZMK elisa kit :: Mouse Granzyme K (GZMK) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2018976 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $265 +1 FREE 8GB USB
48-Strip-Wells  /  $490 +1 FREE 8GB USB
96-Strip-Wells  /  $655 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,660 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,795 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Granzyme K (GZMK), ELISA Kit

★Popular Item★
 Also Known As   

Granzyme K (GZMK) ELISA Kit

 Product Synonym Names    GZM-K; TRYP2; PRSS; Serine Protease,Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2
 Product Gene Name   

GZMK elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600784
 3D Structure    ModBase 3D Structure for P49863
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of GZMK. No significant cross-reactivity or interference between GZMK and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, cell lysates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    15.6-1,000pg/mL
 Sensitivity    6.3pg/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GZMK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GZMK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of GZMK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for GZMK purchase    MBS2018976 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Granzyme K (GZMK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1,000pg/mL in biological research samples containing GZMK, with an estimated sensitivity of 6.3pg/mL. The ELISA analytical biochemical technique of the MBS2018976 kit is based on GZMK antibody-GZMK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GZMK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GZMK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell lysates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for GZMK elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GZMK in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GZMK. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GZMK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GZMK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of GZMK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for GZMK elisa kit    Enzyme & Kinase; Tumor immunity; Infection immunity; Immune molecule
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 Typical Testing Data/Standard Curve (for reference only) of GZMK elisa kit    GZMK elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2018976. Click to request current manual
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NCBI/Uniprot data below describe general gene information for GZMK. It may not necessarily be applicable to this product.
 NCBI GI #    4504235
 NCBI GeneID    3003
 NCBI Accession #    NP_002095.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002104.2 [Other Products]
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 UniProt Primary Accession #    P49863 [Other Products]
 UniProt Secondary Accession #    B2R563 [Other Products]
 UniProt Related Accession #    P49863 [Other Products]
 Molecular Weight    28,882 Da
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 NCBI Official Full Name    granzyme K
 NCBI Official Synonym Full Names    granzyme K (granzyme 3; tryptase II)
 NCBI Official Symbol    GZMK [Similar Products]
 NCBI Official Synonym Symbols   
TRYP2
[Similar Products]
 NCBI Protein Information    granzyme K
 UniProt Protein Name    Granzyme K
 UniProt Synonym Protein Names   
Fragmentin-3; Granzyme-3; NK-tryptase-2; NK-Tryp-2
 Protein Family    Granzyme
 UniProt Gene Name    GZMK [Similar Products]
 UniProt Synonym Gene Names    TRYP2; NK-Tryp-2 [Similar Products]
 UniProt Entry Name    GRAK_HUMAN
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 NCBI Summary for GZMK    This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GZMK    GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.21.-; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis
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 Research Articles on GZMK    1. Extracellular GrK is an unexpected direct modulator of lipopolysaccharide-TLR4 signaling during the antimicrobial innate immune response.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with GZMK elisa kitDiseases associated with GZMK elisa kit
 Reference Product  PubMed Publications
 GZMB elisa kit  >36 publications with GZMK and GZMB
 GZMA elisa kit  >12 publications with GZMK and GZMA
 PRF1 elisa kit  >9 publications with GZMK and PRF1
 GZMH elisa kit  >2 publications with GZMK and GZMH
 CCR5 elisa kit  >1 publications with GZMK and CCR5
 CD2 elisa kit  >1 publications with GZMK and CD2
 Disease Name  Pubmed Publications
 Death Antibodies  >14 publications with GZMK and Death
 Inflammation Antibodies  >4 publications with GZMK and Inflammation
 Lung Diseases Antibodies  >1 publications with GZMK and Lung Diseases
 Heart Diseases Antibodies  >1 publications with GZMK and Heart Diseases
 Pneumonia Antibodies  >1 publications with GZMK and Pneumonia
 Depressive Disorder Antibodies  >1 publications with GZMK and Depressive Disorder
 Necrosis Antibodies  >1 publications with GZMK and Necrosis
Organs/Tissues associated with GZMK elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >26 publications with GZMK and Blood
 Lung Antibodies  >3 publications with GZMK and Lung
 Brain Antibodies  >1 publications with GZMK and Brain
 Thymus Antibodies  >1 publications with GZMK and Thymus
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