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H2AFX elisa kit :: Mouse H2A histone family, member X ELISA Kit

Scan QR to view Datasheet Catalog #    MBS907486 H2AFX elisa kit
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

H2A histone family, member X (H2AFX), ELISA Kit

 Also Known As   

Mouse Histone H2A.x, H2AFX ELISA Kit

 Product Synonym Names    Mouse Histone H2A.x (H2AFX) ELISA kit; H2A.X; H2A/X; H2AX; H2AX histone; H2A histone family; member X
 Product Gene Name   

H2AFX elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P27661
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse H2AFX. No significant cross-reactivity or interference between mouse H2AFX and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    15.6 pg/ml-1000 pg/ml.
 Sensitivity    The minimum detectable dose of mouse H2AFX is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of H2AFX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for H2AFX purchase    MBS907486 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the H2A histone family, member X (H2AFX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml-1000 pg/ml in biological research samples containing H2AFX, with an estimated sensitivity of The minimum detectable dose of mouse H2AFX is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS907486 kit is based on H2AFX antibody-H2AFX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect H2AFX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, H2AFX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for H2AFX elisa kit

   Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for H2AFX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any H2AFX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H2AFX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H2AFX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS907486. Click to request current manual
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NCBI/Uniprot data below describe general gene information for H2AFX. It may not necessarily be applicable to this product.
 NCBI GI #    7106331
 NCBI GeneID    15270
 NCBI Accession #    NP_034566.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_010436.2 [Other Products]
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 UniProt Primary Accession #    P27661 [Other Products]
 UniProt Related Accession #    P27661 [Other Products]
 Molecular Weight    15,143 Da
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 NCBI Official Full Name    histone H2AX
 NCBI Official Synonym Full Names    H2A histone family, member X
 NCBI Official Symbol    H2afx [Similar Products]
 NCBI Official Synonym Symbols   
H2ax; H2A.X; AW228881; Hist5-2ax; gammaH2ax
[Similar Products]
 NCBI Protein Information    histone H2AX; H2a/x; histone H2A.x; histone 5 protein 2ax
 UniProt Protein Name    Histone H2AX
 UniProt Synonym Protein Names   
Histone H2A.X
 UniProt Gene Name    H2afx [Similar Products]
 UniProt Synonym Gene Names    H2a.x; H2ax; Hist5-2ax; H2a/x [Similar Products]
 UniProt Entry Name    H2AX_MOUSE
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 UniProt Comments for H2AFX    H2AX: a histone that replaces conventional H2A in a subset of nucleosomes. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Phosphorylated on S139 by ATM and DNA-PK in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Dephosphorylation of S139 by PP2A is required for DNA DSB repair. Apparently phosphorylated on Y143 by WSTF, determining the relative recruitment of either DNA repair or pro-apoptotic factors. H2AXpY142 favors the recruitment of pro-apoptotic factors APBB1 and JNK1. In contrast, dephosphorylation of pY143 by EYA phosphatases favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated pS139. Monoubiquitination of K119 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'K63' linkages by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage.

Protein type: DNA repair, damage; Helicase; DNA-binding

Cellular Component: XY body; male germ cell nucleus; chromosome, telomeric region; condensed nuclear chromosome; nuclear chromatin; replication fork; chromosome; nucleosome; chromatin; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; histone binding; protein heterodimerization activity; damaged DNA binding

Biological Process: meiotic cell cycle; DNA damage checkpoint; spermatogenesis; cell cycle; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination; DNA recombination
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 Research Articles on H2AFX    1. H2ax functions in a haploinsufficient manner to suppress allelic imbalances and limit molecular heterogeneity within and among Emu-c-Myc lymphomas.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with H2AFX elisa kitDiseases associated with H2AFX elisa kit
 Products by Pathway  Pathway Diagram
 ATM Mediated Phosphorylation Of Repair Proteins Pathway antibodies  ATM Mediated Phosphorylation Of Repair Proteins Pathway Diagram
 ATM Mediated Response To DNA Double-strand Break Pathway antibodies  ATM Mediated Response To DNA Double-strand Break Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway antibodies  Assembly Of The RAD50-MRE11-NBS1 Complex At DNA Double-strand Breaks Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Double-Strand Break Repair Pathway antibodies  Double-Strand Break Repair Pathway Diagram
 Homologous Recombination Repair Pathway antibodies  Homologous Recombination Repair Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >376 publications with H2AFX and Neoplasms
 Ataxia Antibodies  >250 publications with H2AFX and Ataxia
 Carcinoma Antibodies  >121 publications with H2AFX and Carcinoma
 Drug Toxicity Antibodies  >84 publications with H2AFX and Drug Toxicity
 Skin Diseases Antibodies  >68 publications with H2AFX and Skin Diseases
 Breast Neoplasms Antibodies  >66 publications with H2AFX and Breast Neoplasms
 Gastrointestinal Diseases Antibodies  >48 publications with H2AFX and Gastrointestinal Diseases
 Nervous System Diseases Antibodies  >45 publications with H2AFX and Nervous System Diseases
 Lung Neoplasms Antibodies  >44 publications with H2AFX and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >42 publications with H2AFX and Neoplasms, Experimental
Organs/Tissues associated with H2AFX elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >63 publications with H2AFX and Lung
 Brain Antibodies  >29 publications with H2AFX and Brain
 Bone Antibodies  >23 publications with H2AFX and Bone
 Liver Antibodies  >15 publications with H2AFX and Liver
 Testis Antibodies  >11 publications with H2AFX and Testis
 Heart Antibodies  >11 publications with H2AFX and Heart
 Intestine Antibodies  >10 publications with H2AFX and Intestine
 Eye Antibodies  >9 publications with H2AFX and Eye
 Embryonic Tissue Antibodies  >9 publications with H2AFX and Embryonic Tissue
 Ovary Antibodies  >6 publications with H2AFX and Ovary
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