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HB-EGF elisa kit :: Mouse Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS267401
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit

 Product Gene Name   

HB-EGF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HB-EGF concentration
 Assay Type    Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HB-EGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HB-EGF purchase    MBS267401 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heparin Binding Epidermal Growth Factor Like Growth Factor (HB-EGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing HB-EGF, with an estimated sensitivity of 5 pg/ml. The ELISA analytical biochemical technique of the MBS267401 kit is based on HB-EGF antibody-HB-EGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HB-EGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HB-EGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HB-EGF concentration. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for HB-EGF elisa kit

   Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse HB-EGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of HB-EGF elisa kit    HB-EGF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS267401. Click to request current manual
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NCBI/Uniprot data below describe general gene information for HB-EGF. It may not necessarily be applicable to this product.
 NCBI GI #    1236400
 NCBI GeneID    15200
 NCBI Accession #    AAC52617.1 [Other Products]
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 UniProt Related Accession #    Q06186 [Other Products]
 Molecular Weight    22,808 Da
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 NCBI Official Full Name    heparin-binding epidermal growth factor-like growth factor
 NCBI Official Synonym Full Names    heparin-binding EGF-like growth factor
 NCBI Official Symbol    Hbegf [Similar Products]
 NCBI Official Synonym Symbols   
Dtr; Dts; Hegfl; AW047313
[Similar Products]
 NCBI Protein Information    proheparin-binding EGF-like growth factor; diphtheria toxin receptor; heparin binding epidermal growth factor-like growth factor
 UniProt Protein Name    Proheparin-binding EGF-like growth factor
 UniProt Gene Name    Hbegf [Similar Products]
 UniProt Synonym Gene Names    Dtr; Hegfl; HB-EGF; HBEGF [Similar Products]
 UniProt Entry Name    HBEGF_MOUSE
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 UniProt Comments for HB-EGF    HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: heparin binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; positive regulation of protein kinase B signaling cascade; cell migration; negative regulation of elastin biosynthetic process; positive regulation of smooth muscle cell proliferation; blastocyst growth; angiogenesis; positive regulation of cell growth; regulation of heart contraction; positive regulation of cell migration; wound healing, spreading of epidermal cells
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 Research Articles on HB-EGF    1. Decreased nitric oxide bioavailability leads to increased HB-EGF expression, which may be an important mediator of the resulting progressive diabetic nephropathy in eNOS-knockout diabetic mice.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with HB-EGF elisa kitPathways associated with HB-EGF elisa kit
 Reference Product  PubMed Publications
 Egfr elisa kit  >18 publications with HB-EGF and Egfr
 Nrg1 elisa kit  >1 publications with HB-EGF and Nrg1
 Nrg2 elisa kit  >1 publications with HB-EGF and Nrg2
 Erbb3 elisa kit  >1 publications with HB-EGF and Erbb3
 Adam17 elisa kit  >1 publications with HB-EGF and Adam17
 Erbb4 elisa kit  >1 publications with HB-EGF and Erbb4
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 EGFR Transactivation By Gastrin Pathway antibodies  EGFR Transactivation By Gastrin Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
Diseases associated with HB-EGF elisa kitOrgans/Tissues associated with HB-EGF elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with HB-EGF and Neoplasms
 Cardiovascular Diseases Antibodies  >8 publications with HB-EGF and Cardiovascular Diseases
 Inflammation Antibodies  >6 publications with HB-EGF and Inflammation
 Necrosis Antibodies  >6 publications with HB-EGF and Necrosis
 Heart Diseases Antibodies  >4 publications with HB-EGF and Heart Diseases
 Drug Toxicity Antibodies  >3 publications with HB-EGF and Drug Toxicity
 Breast Neoplasms Antibodies  >3 publications with HB-EGF and Breast Neoplasms
 Lung Diseases Antibodies  >3 publications with HB-EGF and Lung Diseases
 Disease Models, Animal Antibodies  >3 publications with HB-EGF and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >2 publications with HB-EGF and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >8 publications with HB-EGF and Lung
 Muscle Antibodies  >7 publications with HB-EGF and Muscle
 Embryonic Tissue Antibodies  >6 publications with HB-EGF and Embryonic Tissue
 Brain Antibodies  >5 publications with HB-EGF and Brain
 Bone Antibodies  >4 publications with HB-EGF and Bone
 Skin Antibodies  >4 publications with HB-EGF and Skin
 Heart Antibodies  >4 publications with HB-EGF and Heart
 Eye Antibodies  >2 publications with HB-EGF and Eye
 Joint Antibodies  >1 publications with HB-EGF and Joint
 Prostate Antibodies  >1 publications with HB-EGF and Prostate
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