Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow HNF6alpha rightarrow Mouse HNF6alpha  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Kalirin (KALRN) ELISA Kit
 • Phosphatase 1 (PPP1CC) Recombinant Protein
 • Protease (L3) Recombinant Protein
 • gem (nuclear organelle) associated protein 6 (GEMIN6) ELISA Kit
 • BBS1 Antibody
 • Cortisol-3 Antibody
 • ABCA2 Antibody
 • Tumor Necrosis Factor Ligand Superfamily, Member 4 (TNFSF4) ELISA Kit
 • Specificity protein 1 (SP1) ELISA Kit
 • neuropilin 2 (NRP2) ELISA Kit
 • CACYBP Recombinant Protein
 • I-Ab (public; Ia.m8) Antibody
 • DnaJ homolog subfamily C member 14, DNAJC14 ELISA Kit
 • Tramadol Antigen
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

HNF6alpha elisa kit :: Mouse Hepatocyte Nuclear Factor 6 Alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS729652
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hepatocyte Nuclear Factor 6 Alpha (HNF6alpha), ELISA Kit

 Also Known As   

Mouse Hepatocyte Nuclear Factor 6 Alpha ELISA Kit

 Product Gene Name   

HNF6alpha elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    604164
Section Bar
 Species Reactivity    Mouse
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    100-2500pg/mL
 Sensitivity    1.0pg/mL
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of HNF6alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for HNF6alpha purchase    MBS729652 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatocyte Nuclear Factor 6 Alpha (HNF6alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100-2500pg/mL in biological research samples containing HNF6alpha, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS729652 kit is based on HNF6alpha antibody-HNF6alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HNF6alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HNF6alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for HNF6alpha elisa kit

   Intended Uses: This HNF6a ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHNF6a in the sample, thisHNF6a ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHNF6a concentration. The concentration ofHNF6a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This HNF6a enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHNF6a. Standards or samples are then added to the microtiter plate wells andHNF6a if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHNF6a present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHNF6a are added to each well to "sandwich" theHNF6a immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHNF6a and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
 Product Categories/Family for HNF6alpha elisa kit    Epigenetics and Nuclear Signaling
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of HNF6alpha elisa kit    HNF6alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS729652. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for HNF6alpha. It may not necessarily be applicable to this product.
 NCBI GI #    4100316
 NCBI GeneID    3175
 NCBI Accession #    AAD00826.1 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q99744; Q9UMR6; B2RTV4 [Other Products]
 UniProt Related Accession #    Q9UBC0 [Other Products]
 Molecular Weight    51,023 Da
Table BarTOPTable Bar
 NCBI Official Full Name    hepatocyte nuclear factor-6 alpha
 NCBI Official Synonym Full Names    one cut homeobox 1
 NCBI Official Symbol    ONECUT1 [Similar Products]
 NCBI Official Synonym Symbols   
HNF6; HNF-6; HNF6A
[Similar Products]
 NCBI Protein Information    hepatocyte nuclear factor 6; one cut domain family member 1; hepatocyte nuclear factor 6, alpha
 UniProt Protein Name    Hepatocyte nuclear factor 6
 UniProt Synonym Protein Names   
One cut domain family member 1; One cut homeobox 1
 UniProt Gene Name    ONECUT1 [Similar Products]
 UniProt Synonym Gene Names    HNF6; HNF6A; HNF-6 [Similar Products]
 UniProt Entry Name    HNF6_HUMAN
Section Bar
 NCBI Summary for HNF6alpha    This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
Section Bar
 UniProt Comments for HNF6alpha    ONECUT1: Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. Belongs to the CUT homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: spleen development; cell fate commitment; transcription, DNA-dependent; regulation of cell-matrix adhesion; system development; glucose metabolic process; liver development; endocrine pancreas development; regulation of transcription, DNA-dependent; B cell differentiation; epithelial cell development; cilium biogenesis; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; endoderm development
Table BarTOPTable Bar
 Research Articles on HNF6alpha    1. The negative correlation between HNF6 expression and pancreatic cancer progression suggests that HNF6 maintains pancreatic epithelial homeostasis, and that its loss contributes to the progression from intraepithelial neoplasms to ductal adenocarcinoma.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with HNF6alpha elisa kitPathways associated with HNF6alpha elisa kit
 Reference Product  PubMed Publications
 FOXA2 elisa kit  >24 publications with HNF6alpha and FOXA2
 PDX1 elisa kit  >8 publications with HNF6alpha and PDX1
 NEUROG3 elisa kit  >6 publications with HNF6alpha and NEUROG3
 ONECUT3 elisa kit  >5 publications with HNF6alpha and ONECUT3
 NKX6-1 elisa kit  >3 publications with HNF6alpha and NKX6-1
 CREBBP elisa kit  >2 publications with HNF6alpha and CREBBP
 CCND1 elisa kit  >1 publications with HNF6alpha and CCND1
 FGF10 elisa kit  >1 publications with HNF6alpha and FGF10
 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FOXM1 Transcription Factor Network Pathway antibodies  FOXM1 Transcription Factor Network Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Regulation Of Beta-cell Development Pathway antibodies  Regulation Of Beta-cell Development Pathway Diagram
 Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Early Pancreatic Precursor Cells Pathway Diagram
 Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
Diseases associated with HNF6alpha elisa kitOrgans/Tissues associated with HNF6alpha elisa kit
 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >14 publications with HNF6alpha and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >9 publications with HNF6alpha and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >9 publications with HNF6alpha and Liver Neoplasms
 Disease Models, Animal Antibodies  >9 publications with HNF6alpha and Disease Models, Animal
 Hematologic Diseases Antibodies  >4 publications with HNF6alpha and Hematologic Diseases
 Liver Neoplasms, Experimental Antibodies  >2 publications with HNF6alpha and Liver Neoplasms, Experimental
 Kidney Diseases Antibodies  >2 publications with HNF6alpha and Kidney Diseases
 Liver Cirrhosis Antibodies  >2 publications with HNF6alpha and Liver Cirrhosis
 Neoplasms, Experimental Antibodies  >2 publications with HNF6alpha and Neoplasms, Experimental
 Fatty Liver Antibodies  >2 publications with HNF6alpha and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >7 publications with HNF6alpha and Eye
 Bone Antibodies  >2 publications with HNF6alpha and Bone
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions