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Hmgb1 elisa kit :: Mouse High mobility group protein B1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS762757
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

High mobility group protein B1 (Hmgb1), ELISA Kit

 Also Known As   

Mouse High mobility group protein B1 ELISA Kit

 Product Synonym Names    Hmgb1/HMG-1/Amphoterin/high mobility group box 1/High mobility group protein 1/high-mobility group (nonhistone chromosomal) protein 1/high-mobility group box 1/HMG-1/HMG1DKFZp686A04236/HMG3/SBP-1/Sulfoglucuronyl carbohydrate binding protein
 Product Gene Name   

Hmgb1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 MBS762757 QC Report    QC Report PDF
 OMIM    163905
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Hmgb1. No significant cross-reactivity or interference between Hmgb1 and analogues was observed.
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 Samples    Serum, plasma and other biological fluids.
 Assay Type    Sandwich ELISA, Double Antibody
 Detection Range    15.625-1000pg/ml
 Sensitivity    9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hmgb1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hmgb1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay.
 Other Notes    Small volumes of Hmgb1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Hmgb1 purchase    MBS762757 is a ready-to-use microwell, strip plate Sandwich, Double Antibody ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the High mobility group protein B1 (Hmgb1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/ml in biological research samples containing Hmgb1, with an estimated sensitivity of 9.375pg/ml. The ELISA analytical biochemical technique of the MBS762757 kit is based on Hmgb1 antibody-Hmgb1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hmgb1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Hmgb1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Hmgb1 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Hmgb1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Hmgb1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Hmgb1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Hmgb1 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of Hmgb1 elisa kit    Hmgb1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS762757
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NCBI/Uniprot data below describe general gene information for Hmgb1. It may not necessarily be applicable to this product.
 NCBI GI #    926459557
 NCBI GeneID    3146
 NCBI Accession #    NP_001300822.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001313893.1 [Other Products]
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 UniProt Secondary Accession #    Q14321; Q5T7C3; Q6IBE1; A5D8W9 [Other Products]
 UniProt Related Accession #    P09429 [Other Products]
 Molecular Weight    24,894 Da
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 NCBI Official Full Name    high mobility group protein B1
 NCBI Official Synonym Full Names    high mobility group box 1
 NCBI Official Symbol    HMGB1 [Similar Products]
 NCBI Official Synonym Symbols   
HMG1; HMG3; HMG-1; SBP-1
[Similar Products]
 NCBI Protein Information    high mobility group protein B1
 UniProt Protein Name    High mobility group protein B1
 UniProt Synonym Protein Names   
High mobility group protein 1; HMG-1
 Protein Family    High mobility group protein
 UniProt Gene Name    HMGB1 [Similar Products]
 UniProt Synonym Gene Names    HMG1; HMG-1 [Similar Products]
 UniProt Entry Name    HMGB1_HUMAN
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 NCBI Summary for Hmgb1    This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
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 UniProt Comments for Hmgb1    HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: cell surface; condensed chromosome; cytosol; early endosome; ER-Golgi intermediate compartment; extracellular region; extracellular space; nucleoplasm; nucleus; plasma membrane; transcriptional repressor complex

Molecular Function: bubble DNA binding; C-X-C chemokine binding; chemoattractant activity; cytokine activity; damaged DNA binding; DNA bending activity; double-stranded DNA binding; double-stranded RNA binding; four-way junction DNA binding; lipopolysaccharide binding; lyase activity; phosphatidylserine binding; protein binding; RAGE receptor binding; single-stranded DNA binding; single-stranded RNA binding; transcription factor activity; transcription factor binding

Biological Process: activation of innate immune response; apoptosis; apoptotic cell clearance; autophagy; base-excision repair; cell structure disassembly during apoptosis; chromatin assembly; dendritic cell chemotaxis; DNA fragmentation during apoptosis; DNA geometric change; DNA ligation during DNA repair; DNA recombination; DNA topological change; elevation of cytosolic calcium ion concentration; endothelial cell proliferation; inflammatory response; inflammatory response to antigenic stimulus; innate immune response; macrophage activation during immune response; myeloid dendritic cell activation; negative regulation of blood vessel endothelial cell migration; negative regulation of CD4-positive, alpha beta T cell differentiation; negative regulation of interferon-gamma production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; neurite development; plasmacytoid dendritic cell activation; positive chemotaxis; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA binding; positive regulation of DNA ligation; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-1 secretion; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of MAPKKK cascade; positive regulation of mismatch repair; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; programmed cell death; regulation of autophagy; regulation of restriction endodeoxyribonuclease activity; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; regulation of transcription from RNA polymerase II promoter; T-helper 1 cell differentiation; V(D)J recombination
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 Research Articles on Hmgb1    1. HMGB1 enhances the inflammatory responses of HBECs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Hmgb1 elisa kitPathways associated with Hmgb1 elisa kit
 Reference Product  PubMed Publications
 TLR4 elisa kit  >448 publications with Hmgb1 and TLR4
 TLR2 elisa kit  >169 publications with Hmgb1 and TLR2
 RELA elisa kit  >27 publications with Hmgb1 and RELA
 TBP elisa kit  >13 publications with Hmgb1 and TBP
 AGER elisa kit  >10 publications with Hmgb1 and AGER
 TP53 elisa kit  >9 publications with Hmgb1 and TP53
 CREBBP elisa kit  >3 publications with Hmgb1 and CREBBP
 NFKB1 elisa kit  >3 publications with Hmgb1 and NFKB1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Diseases associated with Hmgb1 elisa kitOrgans/Tissues associated with Hmgb1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1610 publications with Hmgb1 and Inflammation
 Necrosis Antibodies  >873 publications with Hmgb1 and Necrosis
 Sepsis Antibodies  >562 publications with Hmgb1 and Sepsis
 Disease Models, Animal Antibodies  >464 publications with Hmgb1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >284 publications with Hmgb1 and Nervous System Diseases
 Liver Diseases Antibodies  >221 publications with Hmgb1 and Liver Diseases
 Heart Diseases Antibodies  >152 publications with Hmgb1 and Heart Diseases
 Myocardial Ischemia Antibodies  >124 publications with Hmgb1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >107 publications with Hmgb1 and Kidney Diseases
 Fibrosis Antibodies  >99 publications with Hmgb1 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1331 publications with Hmgb1 and Blood
 Liver Antibodies  >492 publications with Hmgb1 and Liver
 Lung Antibodies  >455 publications with Hmgb1 and Lung
 Brain Antibodies  >369 publications with Hmgb1 and Brain
 Vascular Antibodies  >361 publications with Hmgb1 and Vascular
 Muscle Antibodies  >214 publications with Hmgb1 and Muscle
 Heart Antibodies  >205 publications with Hmgb1 and Heart
 Kidney Antibodies  >190 publications with Hmgb1 and Kidney
 Bone Antibodies  >154 publications with Hmgb1 and Bone
 Intestine Antibodies  >115 publications with Hmgb1 and Intestine
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