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IAA elisa kit :: Mouse IAA ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2506102
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IAA, ELISA Kit

★Popular Item★
 Also Known As   

Mouse IAA (Insulin Autoantibodies) ELISA Kit

 Product Gene Name   

IAA elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2506102 COA    COA PDF
 3D Structure    ModBase 3D Structure for Q9FLN8
 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinantMouse IAA. No significant cross-reactivity or interference between Mouse IAA and analogues was observed.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    Min: 0.094ng/mL; Max: 10ng/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IAA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IAA purchase    MBS2506102 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IAA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing IAA, with an estimated sensitivity of Min: 0.094ng/mL; Max: 10ng/mL. The ELISA analytical biochemical technique of the MBS2506102 kit is based on IAA antibody-IAA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IAA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IAA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IAA elisa kit

   Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse IAA concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to IAA. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for IAA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IAA, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IAA.You can calculate the concentration of IAA in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of IAA elisa kit    IAA elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2506102. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IAA. It may not necessarily be applicable to this product.
 NCBI GI #    145334821
 NCBI GeneID    835618
 NCBI Accession #    NP_001078756.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001085287.1 [Other Products]
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 UniProt Primary Accession #    Q9FLN8 [Other Products]
 UniProt Secondary Accession #    Q56ZV4; Q8LAR1; A8MS18 [Other Products]
 UniProt Related Accession #    Q9FLN8 [Other Products]
 Molecular Weight    38,070 Da
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 NCBI Official Full Name    IAA carboxylmethyltransferase 1
 NCBI Official Symbol    IAMT1 [Similar Products]
 NCBI Official Synonym Symbols   
IAA carboxylmethyltransferase 1; IAMT1; MCO15.20; MCO15_20
[Similar Products]
 NCBI Protein Information    IAA carboxylmethyltransferase 1
 UniProt Protein Name    Indole-3-acetate O-methyltransferase 1
 UniProt Synonym Protein Names   
IAA carboxylmethyltransferase 1; S-adenosyl-L-methionine:(indol-3-yl) acetate carboxylmethyltransferase 1
 Protein Family    IAA acetyltransferase
 UniProt Gene Name    IAMT1 [Similar Products]
 UniProt Entry Name    IAMT1_ARATH
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 UniProt Comments for IAA    Catalyzes the methylation of the free carboxyl end of the plant hormone indole-3-acetic acid (IAA). Converts IAA to IAA methyl ester (MeIAA). Regulates IAA activities by IAA methylation. Methylation of IAA plays an important role in regulating plant development and auxin homeostasis. Required for correct leaf pattern formation. MeIAA seems to be an inactive form of IAA.
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 Research Articles on IAA    1. Data show that the expression of TCP4 and IAMT1 that encodes a methyltransferase converting free indole-3-acetic acid (IAA) to methyl-IAA ester (MeIAA) was induced and the induction level was dependent on the abundance of TaWRKY71-1 transcripts.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IAA elisa kitPathways associated with IAA elisa kit
 Reference Product  PubMed Publications
 AT1G27340 elisa kit  >2 publications with IAA and AT1G27340
 Products by Pathway  Pathway Diagram
 Gibberellin Inactivation II (methylation) Pathway antibodies  Gibberellin Inactivation II (methylation) Pathway Diagram
 Methyl Indole-3-acetate Interconversion Pathway antibodies  Methyl Indole-3-acetate Interconversion Pathway Diagram
 Methyl Indole-3-acetate Interconversion Pathway antibodies  Methyl Indole-3-acetate Interconversion Pathway Diagram
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