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IL-1beta elisa kit :: Mouse IL-1beta ELISA Kit

Scan QR to view Datasheet Catalog #    MBS355354
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $440 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-1beta, ELISA Kit

★Popular Item★
 Also Known As   

IL-1beta Mouse ELISA Kit

 Product Synonym Names    Mouse IL-1beta
 Product Gene Name   

IL-1beta elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 2; NC_000068.7 (129364579..129371120, complement). Location: 2 F; 2 62.97 cM
 3D Structure    ModBase 3D Structure for P10749
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 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    5 pg/ml-100 pg/ml
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 Preparation and Storage    Store at 2-8 degree C for 6 months.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-1beta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-1beta purchase    MBS355354 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-1beta, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5 pg/ml-100 pg/ml in biological research samples containing IL-1beta. The ELISA analytical biochemical technique of the MBS355354 kit is based on IL-1beta antibody-IL-1beta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1beta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1beta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-1beta elisa kit

  
Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-IL-1betaantibody was pre-coated onto 96-well plates. And the HRP conjugated anti-IL-1beta antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Pathak et al. (2011) proposed that IL1B blockade may be a viable therapy for patients with autoimmune inner ear disease or sensorineural hearing loss that fail to respond to corticosteroids.
 Applications Tested/Suitable for IL-1beta elisa kit   

Quantitative sELISA (EIA)

 Application Notes for IL-1beta elisa kit    For quantitative detection of IL-1beta in mouse serum, plasma, urine, cell culture supernatant or tissue samples.
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 Typical Testing Data/Standard Curve (for reference only) of IL-1beta elisa kit    IL-1beta elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS355354. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-1beta. It may not necessarily be applicable to this product.
 NCBI GI #    6680415
 NCBI GeneID    16176
 NCBI Accession #    NP_032387.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008361.3 [Other Products]
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 UniProt Primary Accession #    P10749 [Other Products]
 UniProt Related Accession #    P10749 [Other Products]
 Molecular Weight    30,931 Da
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 NCBI Official Full Name    interleukin-1 beta
 NCBI Official Synonym Full Names    interleukin 1 beta
 NCBI Official Symbol    Il1b [Similar Products]
 NCBI Official Synonym Symbols   
Il-1b; IL-1beta
[Similar Products]
 NCBI Protein Information    interleukin-1 beta; IL-1 beta
 UniProt Protein Name    Interleukin-1 beta
 UniProt Gene Name    Il1b [Similar Products]
 UniProt Synonym Gene Names    IL-1 beta [Similar Products]
 UniProt Entry Name    IL1B_MOUSE
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 NCBI Summary for IL-1beta    The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]
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 UniProt Comments for IL-1beta    Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Subunit structure: Monomer.

Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

Sequence similarities: Belongs to the IL-1 family.
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 Research Articles on IL-1beta    1. COX-2 has a critical role in mediating the diuretic effects of chronic IL-1beta elevation on the kidney
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-1beta elisa kitPathways associated with IL-1beta elisa kit
 Reference Product  PubMed Publications
 Il6 elisa kit  >205 publications with IL-1beta and Il6
 Tnf elisa kit  >200 publications with IL-1beta and Tnf
 Il1a elisa kit  >162 publications with IL-1beta and Il1a
 Ptgs2 elisa kit  >46 publications with IL-1beta and Ptgs2
 Il1r1 elisa kit  >32 publications with IL-1beta and Il1r1
 Casp1 elisa kit  >31 publications with IL-1beta and Casp1
 Nfkb1 elisa kit  >19 publications with IL-1beta and Nfkb1
 Il18 elisa kit  >18 publications with IL-1beta and Il18
 Myd88 elisa kit  >13 publications with IL-1beta and Myd88
 Il1r2 elisa kit  >10 publications with IL-1beta and Il1r2
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
Diseases associated with IL-1beta elisa kitOrgans/Tissues associated with IL-1beta elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >252 publications with IL-1beta and Inflammation
 Necrosis Antibodies  >175 publications with IL-1beta and Necrosis
 Skin Diseases Antibodies  >38 publications with IL-1beta and Skin Diseases
 Stomach Neoplasms Antibodies  >31 publications with IL-1beta and Stomach Neoplasms
 Arthritis, Rheumatoid Antibodies  >26 publications with IL-1beta and Arthritis, Rheumatoid
 Myocardial Ischemia Antibodies  >17 publications with IL-1beta and Myocardial Ischemia
 Adenocarcinoma Antibodies  >17 publications with IL-1beta and Adenocarcinoma
 Hypersensitivity Antibodies  >12 publications with IL-1beta and Hypersensitivity
 Lung Neoplasms Antibodies  >11 publications with IL-1beta and Lung Neoplasms
 Fever Antibodies  >11 publications with IL-1beta and Fever
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >220 publications with IL-1beta and Blood
 Lung Antibodies  >51 publications with IL-1beta and Lung
 Brain Antibodies  >29 publications with IL-1beta and Brain
 Muscle Antibodies  >18 publications with IL-1beta and Muscle
 Bone Marrow Antibodies  >15 publications with IL-1beta and Bone Marrow
 Lymph Node Antibodies  >7 publications with IL-1beta and Lymph Node
 Spleen Antibodies  >6 publications with IL-1beta and Spleen
 Mammary Gland Antibodies  >5 publications with IL-1beta and Mammary Gland
 Vagina Antibodies  >4 publications with IL-1beta and Vagina
 Thymus Antibodies  >3 publications with IL-1beta and Thymus
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