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IL5 elisa kit :: Mouse IL-5 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175807
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96-Strip-Wells  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IL-5 (IL5), ELISA Kit

★Popular Item★
 Also Known As   

Mouse IL-5 PicoKine ELISA Kit

 Product Synonym Names    Interleukin-5; interleukin 5; B cell differentiation factor I; B-cell differentiation factor I; Colony stimulating factor eosinophil; EDF; Eosinophil differentiation factor; IL 5; IL-5; IL5; IL5_HUMAN; Interleukin 5; Interleukin 5 (colony stimulating factor eosinophil); Interleukin-5; Interleukin5; T cell replacing factor; T-cell replacing factor; TRF
 Product Gene Name   

IL5 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P04401
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse IL-5
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 Samples    Cell culture supernates and serum
 Assay Type    Sandwich
 Detection Range    15.6pg/ml-1000pg/ml
 Sensitivity    < 2pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL5 purchase    MBS175807 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-5 (IL5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6pg/ml-1000pg/ml in biological research samples containing IL5, with an estimated sensitivity of < 2pg/ml. The ELISA analytical biochemical technique of the MBS175807 kit is based on IL5 antibody-IL5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates and serum. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL5 elisa kit

   Principle of the assay: mouse IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-5 has been precoated onto 96-well plates. Standards(sf21,M21-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-5 amount of sample captured in plate.
Background: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with thatrecently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils andeosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3Human and mouse of IL-5 share 70% amino acid sequence homology.
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 Typical Testing Data/Standard Curve (for reference only) of IL5 elisa kit    Mouse IL-5 PicoKine ELISA Kit standard curve
IL5 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS175807. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL5. It may not necessarily be applicable to this product.
 NCBI GI #    124342
 NCBI GeneID    16191
 NCBI Accession #    P04401.1 [Other Products]
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 UniProt Primary Accession #    P04401 [Other Products]
 UniProt Related Accession #    P04401 [Other Products]
 Molecular Weight    15,410 Da
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 NCBI Official Full Name    Interleukin-5
 NCBI Official Synonym Full Names    interleukin 5
 NCBI Official Symbol    Il5 [Similar Products]
 NCBI Official Synonym Symbols   
Il-5
[Similar Products]
 NCBI Protein Information    interleukin-5; TRF; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor
 UniProt Protein Name    Interleukin-5
 UniProt Synonym Protein Names   
B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF
 Protein Family    Interleukin
 UniProt Gene Name    Il5 [Similar Products]
 UniProt Synonym Gene Names    Il-5; IL-5; BCGF-II; TRF [Similar Products]
 UniProt Entry Name    IL5_MOUSE
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 UniProt Comments for IL5    IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; immune response; positive regulation of transcription factor activity; positive regulation of immunoglobulin secretion; positive regulation of eosinophil differentiation
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 Research Articles on IL5    1. A decrease in the levels of IL-5, IL-9, and IL-6R in the BALF.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL5 elisa kitDiseases associated with IL5 elisa kit
 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Disease Name  Pubmed Publications
 Hypersensitivity Antibodies  >125 publications with IL5 and Hypersensitivity
 Asthma Antibodies  >108 publications with IL5 and Asthma
 Inflammation Antibodies  >107 publications with IL5 and Inflammation
 Respiratory Hypersensitivity Antibodies  >92 publications with IL5 and Respiratory Hypersensitivity
 Lung Diseases Antibodies  >88 publications with IL5 and Lung Diseases
 Eosinophilia Antibodies  >67 publications with IL5 and Eosinophilia
 Necrosis Antibodies  >58 publications with IL5 and Necrosis
 Fibrosis Antibodies  >13 publications with IL5 and Fibrosis
 Bronchial Hyperreactivity Antibodies  >13 publications with IL5 and Bronchial Hyperreactivity
 Hyperplasia Antibodies  >10 publications with IL5 and Hyperplasia
Organs/Tissues associated with IL5 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >329 publications with IL5 and Blood
 Spleen Antibodies  >29 publications with IL5 and Spleen
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