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IL6 elisa kit :: Mouse IL-6 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS824703
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $470 +1 FREE 8GB USB
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 Product Name   

IL-6 (IL6), ELISA Kit

★Popular Item★
 Also Known As   

Mouse IL-6 ELISA Kit

 Product Synonym Names    IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
 Product Gene Name   

IL6 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P08505
 Species Reactivity    Mouse
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 Specificity    The Mouse IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-6 proteins within the range of 7.8 pg/ml - 500 pg/ml.
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 Samples    Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
 Sensitivity    4 pg/ml.
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 Preparation and Storage    Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL6 purchase    MBS824703 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6 (IL6) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL6, with an estimated sensitivity of 4 pg/ml. The ELISA analytical biochemical technique of the MBS824703 kit is based on IL6 antibody-IL6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL6 elisa kit

   Principle of the Assay: Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kitis an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-6 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Interleukin 6 is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hematopoiesis. It is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells. IL-6 is a prototypic member of the IL-6 superfamily of cytokines that share gp130 as a component required for signal transduction. The mouse, rat and human IL-6 cDNAs have been cloned. The mouse IL-6 cDNA encodes a 211 amino acid residue precursor polypeptide with a hydrophobic signal sequence that is cleaved to generate the 187 aa residue mature protein. Mouse to rat and human, there is approximately 87% and 39% aa identity, respectively. Although human and mouse IL-6 are equally active on mouse cells, mouse IL-6 is not active on human cells. The high-affinity IL-6 receptor complex, which mediates IL-6 bioactivity, consists of two membrane glycoproteins. The production of IL-6 is upregulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-13 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies.
 Applications Tested/Suitable for IL6 elisa kit   

Sandwich ELISA (SE)

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 Typical Testing Data/Standard Curve (for reference only) of IL6 elisa kit    IL6 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS824703. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL6. It may not necessarily be applicable to this product.
 NCBI GI #    13624311
 NCBI GeneID    16193
 NCBI Accession #    NP_112445.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_031168.1 [Other Products]
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 UniProt Primary Accession #    P08505 [Other Products]
 UniProt Secondary Accession #    Q3UCQ0; Q8BN26 [Other Products]
 UniProt Related Accession #    P08505 [Other Products]
 Molecular Weight    24,384 Da
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 NCBI Official Full Name    interleukin-6
 NCBI Official Synonym Full Names    interleukin 6
 NCBI Official Symbol    Il6 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    interleukin-6; interleukin HP-1; B-cell hybridoma growth factor
 UniProt Protein Name    Interleukin-6
 UniProt Synonym Protein Names   
B-cell hybridoma growth factor; Interleukin HP-1
 Protein Family    Interleukin
 UniProt Gene Name    Il6 [Similar Products]
 UniProt Synonym Gene Names    Il-6; IL-6 [Similar Products]
 UniProt Entry Name    IL6_MOUSE
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 UniProt Comments for IL6    IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; interleukin-6 receptor complex; external side of plasma membrane

Molecular Function: protein binding; interleukin-6 receptor binding; growth factor activity; cytokine activity; receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; negative regulation of cytokine secretion; myeloid cell homeostasis; positive regulation of JAK-STAT cascade; glucose homeostasis; positive regulation of tyrosine phosphorylation of Stat3 protein; muscle maintenance; regulation of apoptosis; regulation of cell shape; positive regulation of T-helper 2 cell differentiation; positive regulation of acute inflammatory response; negative regulation of gluconeogenesis; acute-phase response; positive regulation of T cell proliferation; cell growth; defense response to virus; neurite development; positive regulation of protein import into nucleus, translocation; positive regulation of interleukin-6 production; defense response to protozoan; positive regulation of chemokine production; positive regulation of peptidyl-tyrosine phosphorylation; cell redox homeostasis; positive regulation of B cell activation; negative regulation of protein kinase activity; neutrophil apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of muscle development; positive regulation of translation; T cell activation; negative regulation of hormone secretion; positive regulation of smooth muscle cell proliferation; regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of caspase activity; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; response to wounding; hepatic immune response; inflammatory response; negative regulation of chemokine biosynthetic process; cytokine and chemokine mediated signaling pathway; positive regulation of immunoglobulin secretion; positive regulation of peptidyl-serine phosphorylation; endocrine pancreas development; regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; immune response; positive regulation of neuron differentiation; positive regulation of DNA replication; positive regulation of transmission of nerve impulse
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 Research Articles on IL6    1. It is a pro-inflammatory cytokine.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with IL6 elisa kitDiseases associated with IL6 elisa kit
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2270 publications with IL6 and Inflammation
 Necrosis Antibodies  >1642 publications with IL6 and Necrosis
 Autoimmune Diseases Antibodies  >431 publications with IL6 and Autoimmune Diseases
 Skin Diseases Antibodies  >279 publications with IL6 and Skin Diseases
 Arthritis, Rheumatoid Antibodies  >260 publications with IL6 and Arthritis, Rheumatoid
 Atherosclerosis Antibodies  >230 publications with IL6 and Atherosclerosis
 Myocardial Ischemia Antibodies  >221 publications with IL6 and Myocardial Ischemia
 Ischemia Antibodies  >118 publications with IL6 and Ischemia
 Diabetes Mellitus, Type 2 Antibodies  >103 publications with IL6 and Diabetes Mellitus, Type 2
 Breast Neoplasms Antibodies  >100 publications with IL6 and Breast Neoplasms
Organs/Tissues associated with IL6 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >383 publications with IL6 and Lung
 Bone Marrow Antibodies  >194 publications with IL6 and Bone Marrow
 Eye Antibodies  >119 publications with IL6 and Eye
 Spleen Antibodies  >89 publications with IL6 and Spleen
 Embryonic Tissue Antibodies  >80 publications with IL6 and Embryonic Tissue
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