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Ihh elisa kit :: Mouse Indian hedgehog protein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2886533
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Indian hedgehog protein (Ihh), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Indian hedgehog protein ELISA Kit

 Product Synonym Names    Indian hedgehog protein; IHH; HHG-2; Ihh
 Product Gene Name   

Ihh elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2886533 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P97812
 Species Reactivity    Mouse
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Ihh elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ihh purchase    MBS2886533 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Indian hedgehog protein (Ihh) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ihh. The ELISA analytical biochemical technique of the MBS2886533 kit is based on Ihh antibody-Ihh antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ihh antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ihh. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Ihh elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Ihh elisa kit    Ihh elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2886533. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Ihh. It may not necessarily be applicable to this product.
 NCBI GI #    922304384
 NCBI GeneID    16147
 NCBI Accession #    NP_034674.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010544.3 [Other Products]
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 UniProt Primary Accession #    P97812 [Other Products]
 UniProt Secondary Accession #    Q61724 [Other Products]
 UniProt Related Accession #    P97812 [Other Products]
 Molecular Weight    45,485 Da
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 NCBI Official Full Name    indian hedgehog protein isoform 1 preproprotein
 NCBI Official Synonym Full Names    Indian hedgehog
 NCBI Official Symbol    Ihh [Similar Products]
 NCBI Official Synonym Symbols   
HHG-2
[Similar Products]
 NCBI Protein Information    indian hedgehog protein
 UniProt Protein Name    Indian hedgehog protein
 UniProt Synonym Protein Names   
HHG-2
 Protein Family    Indian hedgehog protein
 UniProt Gene Name    Ihh [Similar Products]
 UniProt Synonym Gene Names    IHH [Similar Products]
 UniProt Entry Name    IHH_MOUSE
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 UniProt Comments for Ihh    IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Cellular Component: extracellular region; membrane; plasma membrane; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; patched binding; peptidase activity; protein binding

Biological Process: bone resorption; camera-type eye development; cartilage development; cell communication; cell differentiation; cell fate specification; cell maturation; cell-cell signaling; embryonic camera-type eye morphogenesis; embryonic development; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic pattern specification; heart looping; in utero embryonic development; intein-mediated protein splicing; morphogenesis of a branching structure; multicellular organism growth; multicellular organismal development; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of eye pigmentation; negative regulation of immature T cell proliferation in the thymus; negative regulation of signal transduction; negative regulation of T cell differentiation in the thymus; neuron development; ossification; osteoblast differentiation; pancreas development; pattern specification process; patterning of blood vessels; positive regulation of alpha-beta T cell differentiation; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; proteoglycan metabolic process; proteolysis; regulation of cell differentiation; regulation of growth; skeletal development; smooth muscle development; smoothened signaling pathway; vasculature development; vitelline membrane formation
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 Research Articles on Ihh    1. increased expression in cell bodies of cutaneous sensory nerves of dorsal root ganglia as compared with skin epithelia
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ihh elisa kitPathways associated with Ihh elisa kit
 Reference Product  PubMed Publications
 Gli1 elisa kit  >88 publications with Ihh and Gli1
 Smo elisa kit  >71 publications with Ihh and Smo
 Gli2 elisa kit  >49 publications with Ihh and Gli2
 Ptch1 elisa kit  >49 publications with Ihh and Ptch1
 Gli3 elisa kit  >43 publications with Ihh and Gli3
 Pthlh elisa kit  >24 publications with Ihh and Pthlh
 Hhip elisa kit  >18 publications with Ihh and Hhip
 Ptch2 elisa kit  >7 publications with Ihh and Ptch2
 Gas1 elisa kit  >3 publications with Ihh and Gas1
 Products by Pathway  Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
Diseases associated with Ihh elisa kitOrgans/Tissues associated with Ihh elisa kit
 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >135 publications with Ihh and Congenital Abnormalities
 Hypertrophy Antibodies  >103 publications with Ihh and Hypertrophy
 Infertility, Male Antibodies  >37 publications with Ihh and Infertility, Male
 Cardiovascular Diseases Antibodies  >25 publications with Ihh and Cardiovascular Diseases
 Brain Diseases Antibodies  >25 publications with Ihh and Brain Diseases
 Liver Diseases Antibodies  >24 publications with Ihh and Liver Diseases
 Brachydactyly type A1 Antibodies  >20 publications with Ihh and Brachydactyly type A1
 Fibrosis Antibodies  >16 publications with Ihh and Fibrosis
 Hypertension Antibodies  >14 publications with Ihh and Hypertension
 Hyperplasia Antibodies  >13 publications with Ihh and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >139 publications with Ihh and Embryonic Tissue
 Liver Antibodies  >61 publications with Ihh and Liver
 Intestine Antibodies  >28 publications with Ihh and Intestine
 Heart Antibodies  >27 publications with Ihh and Heart
 Eye Antibodies  >17 publications with Ihh and Eye
 Extraembryonic Tissue Antibodies  >5 publications with Ihh and Extraembryonic Tissue
 Uncharacterized Tissue Antibodies  >4 publications with Ihh and Uncharacterized Tissue
 Spleen Antibodies  >3 publications with Ihh and Spleen
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