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iNOS elisa kit :: Mouse Inducible nitric oxide synthase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS723353
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Inducible nitric oxide synthase (iNOS), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Inducible nitric oxide synthase ELISA Kit

 Product Gene Name   

iNOS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of iNOS. No significant cross-reactivity or interference between iNOS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between iNOS and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Competitive
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of iNOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for iNOS purchase    MBS723353 is a ready-to-use microwell, strip plate Quantitative Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inducible nitric oxide synthase (iNOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing iNOS, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS723353 kit is based on iNOS antibody-iNOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect iNOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, iNOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for iNOS elisa kit

   Intended Uses: This iNOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse iNOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: iNOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-iNOS antibody and an iNOS-HRP conjugate. The assay sample and buffer are incubated together with iNOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the iNOS concentration since iNOS from samples and iNOS-HRP conjugate compete for the anti-iNOS antibody binding site. Since the number of sites is limited, as more sites are occupied by iNOS from the sample, fewer sites are left to bind iNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The iNOS concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for iNOS elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of iNOS elisa kit    iNOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS723353. Click to request current manual
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NCBI/Uniprot data below describe general gene information for iNOS. It may not necessarily be applicable to this product.
 NCBI GI #    17137626
 NCBI GeneID    35671
 NCBI Accession #    NP_477405.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_058057.6 [Other Products]
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 UniProt Secondary Accession #    Q8IGW1; Q8MR42 [Other Products]
 UniProt Related Accession #    O97477 [Other Products]
 Molecular Weight    62,249 Da
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 NCBI Official Full Name    inos
 NCBI Official Symbol    Inos [Similar Products]
 NCBI Official Synonym Symbols   
bs36h12.y1; CG11143; DmelCG11143; INOS
[Similar Products]
 NCBI Protein Information    CG11143 gene product from transcript CG11143-RA; CG11143-PA; Inos-PA; inos; myo-inositol-1-phosphate-synthase
 UniProt Protein Name    Inositol-3-phosphate synthase
 UniProt Synonym Protein Names   
Myo-inositol 1-phosphate synthase; IPS; MI-1-P synthase
 UniProt Gene Name    Inos [Similar Products]
 UniProt Synonym Gene Names    CG11143; MIP synthase; IPS; MI-1-P synthase [Similar Products]
 UniProt Entry Name    INO1_DROME
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with iNOS elisa kitOrgans/Tissues associated with iNOS elisa kit
 Products by Pathway  Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Synthesis Of IP2, IP, And Ins In The Cytosol Pathway antibodies  Synthesis Of IP2, IP, And Ins In The Cytosol Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4524 publications with iNOS and Blood
 Embryonic Tissue Antibodies  >179 publications with iNOS and Embryonic Tissue
 Head Antibodies  >155 publications with iNOS and Head
 Ovary Antibodies  >92 publications with iNOS and Ovary
 Testis Antibodies  >84 publications with iNOS and Testis
 Whole Body Antibodies  >74 publications with iNOS and Whole Body
 Fat Body Antibodies  >50 publications with iNOS and Fat Body
 Uncharacterized Tissue Antibodies  >2 publications with iNOS and Uncharacterized Tissue
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