Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow IkBKb rightarrow Mouse IkBKb  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Suppressor of cytokine signaling 1 (SOCS1) Recombinant Protein
 • Platelet-Derived Growth Factor AB (PDGF-AB) ELISA Kit
 • Interferon Beta (IFN-beta) ELISA Kit
 • Aryl-hydrocarbon-interactin g protein-like 1 (AIPL1) Antibody
 • alpha-thrombin (F2) Protein
 • Ovalbumin sIgG (OVA sIgG) ELISA Kit
 • Procollagen Type I N-Terminal Propeptide (P I NP) ELISA Kit
 • G Protein Alpha Inhibiting Activity Polypeptide 3 (GNalphaI3... ELISA Kit
 • Thyroid Hormone Receptor, beta-Isotype (THRB) Antibody
 • ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) ELISA Kit
 • EPHB3 Blocking Peptide
 • permeability glycoprotein, P-gp ELISA Kit
 • Interleukin 17A (IL-17A) ELISA Kit
 • O3FAR1 - GPR120 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

IkBKb elisa kit :: Mouse Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2019397 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), ELISA Kit

 Also Known As   

Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) ELISA Kit

 Product Synonym Names    IKK2; NFKBIKB; IKK-beta; IKKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta
 Product Gene Name   

IkBKb elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    603258
 3D Structure    ModBase 3D Structure for O14920
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb).
No significant cross-reactivity or interference between Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) and analogues was observed.
Section Bar
 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.055ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
Section Bar
 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IkBKb elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IkBKb purchase    MBS2019397 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing IkBKb, with an estimated sensitivity of < 0.055ng/mL. The ELISA analytical biochemical technique of the MBS2019397 kit is based on IkBKb antibody-IkBKb antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IkBKb antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IkBKb. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for IkBKb elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for IkBKb elisa kit    Enzyme & Kinase
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of IkBKb elisa kit    IkBKb elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2019397. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for IkBKb. It may not necessarily be applicable to this product.
 NCBI GI #    119583630
 NCBI GeneID    3551
 NCBI Accession #    EAW63226.1 [Other Products]
Section Bar
 UniProt Primary Accession #    O14920 [Other Products]
 UniProt Secondary Accession #    O75327; B4DZ30; B4E0U4 [Other Products]
 UniProt Related Accession #    O14920 [Other Products]
 Molecular Weight    79,509 Da
Table BarTOPTable Bar
 NCBI Official Full Name    inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, isoform CRA_e
 NCBI Official Synonym Full Names    inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
 NCBI Official Symbol    IKBKB [Similar Products]
 NCBI Official Synonym Symbols   
IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
[Similar Products]
 NCBI Protein Information    inhibitor of nuclear factor kappa-B kinase subunit beta
 UniProt Protein Name    Inhibitor of nuclear factor kappa-B kinase subunit beta
 UniProt Synonym Protein Names   
I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
 UniProt Gene Name    IKBKB [Similar Products]
 UniProt Synonym Gene Names    IKKB; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; IKK2; NFKBIKB [Similar Products]
 UniProt Entry Name    IKKB_HUMAN
Section Bar
 NCBI Summary for IkBKb    The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]
Section Bar
 UniProt Comments for IkBKb    IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Protein kinase, Other; Other group; IKK family

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; response to virus; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; negative regulation of apoptosis

Disease: Immunodeficiency 15
Table BarTOPTable Bar
 Research Articles on IkBKb    1. High IKBKB expression is associated with inflammation in heart Valve Diseases.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with IkBKb elisa kitPathways associated with IkBKb elisa kit
 Reference Product  PubMed Publications
 CHUK elisa kit  >1180 publications with IkBKb and CHUK
 RELA elisa kit  >528 publications with IkBKb and RELA
 IKBKG elisa kit  >297 publications with IkBKb and IKBKG
 NFKB1 elisa kit  >47 publications with IkBKb and NFKB1
 NFKBIA elisa kit  >15 publications with IkBKb and NFKBIA
 TNFRSF1A elisa kit  >12 publications with IkBKb and TNFRSF1A
 UBC elisa kit  >6 publications with IkBKb and UBC
 MAP3K14 elisa kit  >5 publications with IkBKb and MAP3K14
 MAP3K7 elisa kit  >4 publications with IkBKb and MAP3K7
 NFKBIB elisa kit  >3 publications with IkBKb and NFKBIB
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with IkBKb elisa kitOrgans/Tissues associated with IkBKb elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1298 publications with IkBKb and Necrosis
 Inflammation Antibodies  >1050 publications with IkBKb and Inflammation
 Insulin Resistance Antibodies  >126 publications with IkBKb and Insulin Resistance
 Breast Neoplasms Antibodies  >126 publications with IkBKb and Breast Neoplasms
 Liver Diseases Antibodies  >116 publications with IkBKb and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >102 publications with IkBKb and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >80 publications with IkBKb and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >69 publications with IkBKb and Lung Neoplasms
 Atherosclerosis Antibodies  >61 publications with IkBKb and Atherosclerosis
 Fibrosis Antibodies  >60 publications with IkBKb and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >740 publications with IkBKb and Blood
 Vascular Antibodies  >277 publications with IkBKb and Vascular
 Lung Antibodies  >267 publications with IkBKb and Lung
 Muscle Antibodies  >230 publications with IkBKb and Muscle
 Liver Antibodies  >220 publications with IkBKb and Liver
 Skin Antibodies  >185 publications with IkBKb and Skin
 Bone Antibodies  >155 publications with IkBKb and Bone
 Brain Antibodies  >155 publications with IkBKb and Brain
 Heart Antibodies  >126 publications with IkBKb and Heart
 Kidney Antibodies  >113 publications with IkBKb and Kidney
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions