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INS elisa kit :: Mouse Insulin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS728172
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Insulin, ELISA Kit

 Also Known As   

Mouse Insulin ELISA Kit

 Product Gene Name   

INS elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 OMIM    613370
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    0.1 ng/mL.
 Intended Uses    This INS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse INS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact technical support.
 Other Notes    Small volumes of INS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for INS purchase    MBS728172 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Insulin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing INS, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS728172 kit is based on INS antibody-INS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect INS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, INS. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for INS elisa kit

   Principle of the Assay: INS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-INS antibody and an INS-HRP conjugate. The assay sample and buffer are incubated together with INS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the INS concentration since INS from samples and INS-HRP conjugate compete for the anti-INS antibody binding site. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of INS elisa kit    INS elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS728172
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NCBI/Uniprot data below describe general gene information for INS. It may not necessarily be applicable to this product.
 NCBI GI #    386828
 NCBI GeneID    3630
 NCBI Accession #    AAA59172.1 [Other Products]
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 UniProt Secondary Accession #    Q5EEX2 [Other Products]
 UniProt Related Accession #    P01308 [Other Products]
 Molecular Weight    21,537 Da
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 NCBI Official Full Name    insulin
 NCBI Official Synonym Full Names    insulin
 NCBI Official Symbol    INS [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2
 UniProt Protein Name    Insulin
 Protein Family    Insulin
 UniProt Gene Name    INS [Similar Products]
 UniProt Entry Name    INS_HUMAN
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 NCBI Summary for INS    After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
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 UniProt Comments for INS    Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; glucose homeostasis; positive regulation of vasodilation; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; glucose transport; regulation of protein secretion; positive regulation of MAPKKK cascade; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; negative regulation of vasodilation; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10
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 Research Articles on INS    1. Data suggest that plasma insulin levels can be modulated by dietary factors; here, plasma insulin levels are up-regulated by sugar-sweetened beverages (in men, increased consumption of 1 serving/day up-regulated plasma insulin).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with INS elisa kitPathways associated with INS elisa kit
 Reference Product  PubMed Publications
 IRS1 elisa kit  >35 publications with INS and IRS1
 FOXO1 elisa kit  >31 publications with INS and FOXO1
 INSR elisa kit  >28 publications with INS and INSR
 IRS2 elisa kit  >26 publications with INS and IRS2
 AKT1 elisa kit  >18 publications with INS and AKT1
 GRB2 elisa kit  >11 publications with INS and GRB2
 IDE elisa kit  >7 publications with INS and IDE
 IGFBP1 elisa kit  >5 publications with INS and IGFBP1
 AKT2 elisa kit  >3 publications with INS and AKT2
 PTPN1 elisa kit  >3 publications with INS and PTPN1
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Arf6 Trafficking Events Pathway antibodies  Arf6 Trafficking Events Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
Diseases associated with INS elisa kitOrgans/Tissues associated with INS elisa kit
 Disease Name  Pubmed Publications
 Metabolic Diseases Antibodies  >1082 publications with INS and Metabolic Diseases
 Diabetes Mellitus Antibodies  >896 publications with INS and Diabetes Mellitus
 Insulin Resistance Antibodies  >327 publications with INS and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >308 publications with INS and Diabetes Mellitus, Type 2
 Obesity Antibodies  >243 publications with INS and Obesity
 Hyperglycemia Antibodies  >221 publications with INS and Hyperglycemia
 Hyperinsulinism Antibodies  >213 publications with INS and Hyperinsulinism
 Hypertension Antibodies  >129 publications with INS and Hypertension
 Polycystic Ovary Syndrome Antibodies  >60 publications with INS and Polycystic Ovary Syndrome
 Fatty Liver Antibodies  >49 publications with INS and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Pancreas Antibodies  >1540 publications with INS and Pancreas
 Blood Antibodies  >1535 publications with INS and Blood
 Brain Antibodies  >767 publications with INS and Brain
 Muscle Antibodies  >560 publications with INS and Muscle
 Liver Antibodies  >416 publications with INS and Liver
 Heart Antibodies  >306 publications with INS and Heart
 Kidney Antibodies  >277 publications with INS and Kidney
 Vascular Antibodies  >277 publications with INS and Vascular
 Nerve Antibodies  >274 publications with INS and Nerve
 Bone Antibodies  >161 publications with INS and Bone
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