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IL-10 elisa kit :: Mouse Interleukin 10 (IL-10) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS268184
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 10 (IL-10), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Interleukin 10 (IL-10) ELISA Kit

 Product Gene Name   

IL-10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 614395
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    1000 pg/ml-15.6 pg/ml
 Sensitivity    Up to 5 pg/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-10 purchase    MBS268184 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 10 (IL-10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 1000 pg/ml-15.6 pg/ml in biological research samples containing IL-10, with an estimated sensitivity of Up to 5 pg/ml. The ELISA analytical biochemical technique of the MBS268184 kit is based on IL-10 antibody-IL-10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for IL-10 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse IL-10 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Testing Data/Standard Curve (for reference only) of IL-10 elisa kit    IL-10 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS268184. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-10. It may not necessarily be applicable to this product.
 NCBI GI #    10835141
 NCBI GeneID    3586
 NCBI Accession #    NP_000563.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000572.2 [Other Products]
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 UniProt Related Accession #    P22301 [Other Products]
 Molecular Weight    20,517 Da
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 NCBI Official Full Name    interleukin-10
 NCBI Official Synonym Full Names    interleukin 10
 NCBI Official Symbol    IL10 [Similar Products]
 NCBI Official Synonym Symbols   
CSIF; TGIF; GVHDS; IL-10; IL10A
[Similar Products]
 NCBI Protein Information    interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
 UniProt Protein Name    Interleukin-10
 UniProt Synonym Protein Names   
Cytokine synthesis inhibitory factor; CSIF
 UniProt Gene Name    IL10 [Similar Products]
 UniProt Synonym Gene Names    IL-10; CSIF [Similar Products]
 UniProt Entry Name    IL10_HUMAN
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 NCBI Summary for IL-10    The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
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 UniProt Comments for IL-10    IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; interleukin-10 receptor binding

Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; leukocyte chemotaxis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; response to molecule of bacterial origin; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; hemopoiesis; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to activity; regulation of sensory perception of pain; receptor biosynthetic process; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To
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 Research Articles on IL-10    1. A meta-analysis indicates that the Interleukin-10-1082 AA genotype is associated with increased overall atherosclerosis risk.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with IL-10 elisa kitPathways associated with IL-10 elisa kit
 Reference Product  PubMed Publications
 CD4 elisa kit  >3691 publications with IL-10 and CD4
 JUN elisa kit  >2091 publications with IL-10 and JUN
 IL6 elisa kit  >586 publications with IL-10 and IL6
 IL4 elisa kit  >397 publications with IL-10 and IL4
 IL2 elisa kit  >223 publications with IL-10 and IL2
 NOS2 elisa kit  >206 publications with IL-10 and NOS2
 IL10RB elisa kit  >38 publications with IL-10 and IL10RB
 IL10RA elisa kit  >20 publications with IL-10 and IL10RA
 DIF elisa kit  >1 publications with IL-10 and DIF
 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Asthma Pathway antibodies  Asthma Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
Diseases associated with IL-10 elisa kitOrgans/Tissues associated with IL-10 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >7221 publications with IL-10 and Necrosis
 Inflammation Antibodies  >5643 publications with IL-10 and Inflammation
 Sepsis Antibodies  >1085 publications with IL-10 and Sepsis
 Colitis Antibodies  >800 publications with IL-10 and Colitis
 Genetic Predisposition to Disease Antibodies  >777 publications with IL-10 and Genetic Predisposition to Disease
 Hypersensitivity, Immediate Antibodies  >746 publications with IL-10 and Hypersensitivity, Immediate
 Inflammatory Bowel Diseases Antibodies  >673 publications with IL-10 and Inflammatory Bowel Diseases
 Arthritis, Rheumatoid Antibodies  >548 publications with IL-10 and Arthritis, Rheumatoid
 Drug Toxicity Antibodies  >502 publications with IL-10 and Drug Toxicity
 Kidney Diseases Antibodies  >420 publications with IL-10 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >14560 publications with IL-10 and Blood
 Spleen Antibodies  >1719 publications with IL-10 and Spleen
 Lung Antibodies  >1641 publications with IL-10 and Lung
 Connective Tissue Antibodies  >203 publications with IL-10 and Connective Tissue
 Uncharacterized Tissue Antibodies  >1 publications with IL-10 and Uncharacterized Tissue
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