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IL-12p35 elisa kit :: Mouse Interleukin 12 p35 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS744303 (PROMOTIONAL PRICING: 10% OFF web price.
Discount will be applied after order has been placed)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 12 p35 (IL-12p35), ELISA Kit

 Also Known As   

Mouse Interleukin 12 p35 ELISA Kit

 Product Gene Name   

IL-12p35 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Samples    Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 pg/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of IL-12p35 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-12p35 purchase    MBS744303 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 12 p35 (IL-12p35) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-12p35, with an estimated sensitivity of 1.0 pg/mL. The ELISA analytical biochemical technique of the MBS744303 kit is based on IL-12p35 antibody-IL-12p35 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-12p35 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-12p35. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture superna tants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-12p35 elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This IL-12P35 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-12P35. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY IL-12P35 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-12P35 antibody and an IL-12P35-HRP conjugate. The assay sample and buffer are incubated together with IL-12P35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12P35 concentration since IL-12P35 from samples and IL-12P35-HRP conjugate compete for the anti-IL-12P35 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12P35 from the sample, fewer sites are left to bind IL-12P35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12P35 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for IL-12p35 elisa kit    Immunology
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 Typical Testing Data/Standard Curve (for reference only) of IL-12p35 elisa kit    IL-12p35 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS744303. Click to request current manual
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NCBI/Uniprot data below describe general gene information for IL-12p35. It may not necessarily be applicable to this product.
 NCBI GI #    113472827
 NCBI Accession #    ABI35901.1 [Other Products]
 Molecular Weight    7,886 Da
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 NCBI Official Full Name    interleukin 12 polypeptide 35, partial
 UniProt Protein Name    Interleukin 12 polypeptide 35
 UniProt Synonym Protein Names   
Interleukin 12 polypeptide 35
 UniProt Gene Name    IL12 [Similar Products]
 UniProt Entry Name    Q0GA55_BUBBU
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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