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IL-35 elisa kit :: Mouse Interleukin 35 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS029510 IL-35 elisa kit
Unit / Price
48-Strip-Wells  /  $435 +1 FREE 8GB USB
96-Strip-Wells  /  $600 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Interleukin 35 (IL-35), ELISA Kit

 Also Known As   

Mouse Interleukin 35 ELISA Kit

 Product Gene Name   

IL-35 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q9Z1X4
 Species Reactivity    Mouse
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 Specificity    No significant cross-reactivity or interference between Mouse IL-35 and analogues was observed.
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 Samples    Serum, Plasma, Tissue Homogenate, Feces and Urine
 Assay Type    Sandwich
 Detection Range    6.25 pg/ml - 200 pg/ml.
 Sensitivity    1.0 pg/ml.
 Intended Uses    This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Intra-assay Precision    Intra-assay CV (%) is less than 15%.
 Inter-assay Precision    Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]
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 Preparation and Storage    Store all reagents at 2-8 degree C
 Other Notes    Small volumes of IL-35 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for IL-35 purchase    MBS029510 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 35 (IL-35) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 6.25 pg/ml - 200 pg/ml in biological research samples containing IL-35, with an estimated sensitivity of 1.0 pg/ml. The ELISA analytical biochemical technique of the MBS029510 kit is based on IL-35 antibody-IL-35 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-35 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-35. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue Homogenate, Feces and Urine. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for IL-35 elisa kit

   Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IL-35 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse IL-35 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-35 and IL-35 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS029510
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NCBI/Uniprot data below describe general gene information for IL-35. It may not necessarily be applicable to this product.
 NCBI GI #    472339106
 NCBI GeneID    16201
 NCBI Accession #    NP_001264250.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001277321.1 [Other Products]
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 UniProt Primary Accession #    Q9Z1X4 [Other Products]
 UniProt Secondary Accession #    Q80VD5; Q812A1; Q8BP80; Q8C2H8; Q8K588 [Other Products]
 UniProt Related Accession #    Q9Z1X4 [Other Products]
 Molecular Weight    96,021 Da
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 NCBI Official Full Name    interleukin enhancer-binding factor 3 isoform 5
 NCBI Official Synonym Full Names    interleukin enhancer binding factor 3
 NCBI Official Symbol    Ilf3 [Similar Products]
 NCBI Official Synonym Symbols   
NF90; NFAR; MBII-26; MPHOSPH4
[Similar Products]
 NCBI Protein Information    interleukin enhancer-binding factor 3; nuclear factor 90; M phase phosphoprotein 4; nuclear factor associated with dsRNA
 UniProt Protein Name    Interleukin enhancer-binding factor 3
 UniProt Gene Name    Ilf3 [Similar Products]
 UniProt Entry Name    ILF3_MOUSE
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 NCBI Summary for IL-35    The protein encoded by this gene contains two double-stranded RNA binding domains and functions in the post-transcriptional regulation of gene expression. It is a component of an RNA-protein complex that may be involved in mediating the export of messenger RNAs. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These isoforms are grouped into two categories, NFAR-1 or NFAR-2, based on variation at the C-terminus. [provided by RefSeq, Mar 2013]
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 UniProt Comments for IL-35    NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nucleolus; RNA-binding

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: enzyme binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: protein amino acid methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation
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 Research Articles on IL-35    1. This work allows to establish a link between Ilf3 and NF90 functions, as RNA binding proteins, and their interacting partners implicated in these functions.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Mouse Interleukin 35 ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS2011667  Recombinant Interleukin 35 (IL35)  MBS2061961  APC-Linked Polyclonal Antibody to Interleukin 35 (IL35)
 MBS2087064  Interleukin 35 (IL35) High Sensitive ELISA Kit  MBS743120  Chicken Interleukin 35 ELISA Kit
 MBS2030934  Recombinant Interleukin 35 (IL35)  MBS2090243  Interleukin 35 (IL35) Antibody Pair
 MBS2061955  APC-Linked Polyclonal Antibody to Interleukin 35 (IL35)  MBS283929  Rat Interleukin 35 (IL-35) ELISA Kit
 MBS008763  Chicken Interleukin 35 ELISA Kit  MBS008967  Cavy Interleukin 35 ELISA Kit
Products associated with IL-35 elisa kitPathways associated with IL-35 elisa kit
 Reference Product  PubMed Publications
 Ilf2 elisa kit  >14 publications with IL-35 and Ilf2
 Xpo5 elisa kit  >5 publications with IL-35 and Xpo5
 Prmt1 elisa kit  >4 publications with IL-35 and Prmt1
 Prkdc elisa kit  >1 publications with IL-35 and Prkdc
 Products by Pathway  Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
Diseases associated with IL-35 elisa kitOrgans/Tissues associated with IL-35 elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >10 publications with IL-35 and Neoplasms
 Skin Diseases Antibodies  >3 publications with IL-35 and Skin Diseases
 Heart Diseases Antibodies  >3 publications with IL-35 and Heart Diseases
 Disease Models, Animal Antibodies  >2 publications with IL-35 and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with IL-35 and Kidney Diseases
 Liver Diseases Antibodies  >2 publications with IL-35 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with IL-35 and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >2 publications with IL-35 and Nervous System Diseases
 Adenocarcinoma Antibodies  >2 publications with IL-35 and Adenocarcinoma
 Inflammation Antibodies  >2 publications with IL-35 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >10 publications with IL-35 and Blood
 Brain Antibodies  >7 publications with IL-35 and Brain
 Liver Antibodies  >6 publications with IL-35 and Liver
 Ovary Antibodies  >4 publications with IL-35 and Ovary
 Kidney Antibodies  >4 publications with IL-35 and Kidney
 Testis Antibodies  >4 publications with IL-35 and Testis
 Lung Antibodies  >3 publications with IL-35 and Lung
 Spleen Antibodies  >2 publications with IL-35 and Spleen
 Thyroid Antibodies  >1 publications with IL-35 and Thyroid
 Fertilized Ovum Antibodies  >1 publications with IL-35 and Fertilized Ovum
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