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Il36g elisa kit :: Mouse Interleukin-36 gamma ELISA Kit

Scan QR to view Datasheet Catalog #    MBS288173
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $325 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,290 +4 FREE 8GB USB
 
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 Product Name   

Interleukin-36 gamma (Il36g), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Interleukin-36 gamma ELISA Kit

 Product Gene Name   

Il36g elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS288173 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q8R460
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse Interleukin-36 gamma
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
 Detection Range    15.6-1000 pg/mL
 Intra-assay Precision    Intra-Assay CV: <=6.5%
 Inter-assay Precision    Inter-Assay CV: <=9.1%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Il36g elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Il36g purchase    MBS288173 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-36 gamma (Il36g) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000 pg/mL in biological research samples containing Il36g. The ELISA analytical biochemical technique of the MBS288173 kit is based on Il36g antibody-Il36g antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Il36g antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Il36g. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Il36g elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of Il36g elisa kit    Il36g elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS288173. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Il36g. It may not necessarily be applicable to this product.
 NCBI GI #    25008588
 NCBI Accession #    Q8R460.1 [Other Products]
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 UniProt Primary Accession #    Q8R460 [Other Products]
 UniProt Secondary Accession #    Q14BB6 [Other Products]
 Molecular Weight    18,733 Da
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 NCBI Official Full Name    Interleukin-36 gamma
 UniProt Protein Name    Interleukin-36 gamma
 UniProt Synonym Protein Names   
Interleukin-1 family member 9; IL-1F9
 Protein Family    Interleukin
 UniProt Gene Name    Il36g [Similar Products]
 UniProt Synonym Gene Names    Il1f9; IL-1F9 [Similar Products]
 UniProt Entry Name    IL36G_MOUSE
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 UniProt Comments for Il36g    Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T-cells and splenocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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