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JAK3 elisa kit :: Mouse Janus kinase 3 (a protein tyrosine kinase, leukocyte) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS900324
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Tyrosine-protein kinase JAK3, JAK3 ELISA Kit

 Product Synonym Names    Mouse Tyrosine-protein kinase JAK3 (JAK3) ELISA kit; JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK; Janus kinase 3 (a protein tyrosine kinase; leukocyte) ; leukocyte Janus kinase; tyrosine-protein kinase JAK3; Janus kinase 3 (a protein tyrosine kinase; leukocyte)
 Product Gene Name   

JAK3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS900324 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q62137
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse JAK3. No significant cross-reactivity or interference between Mouse JAK3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
 Protein Biological Process 1    Immunity
 Protein Biological Process 3    Adaptive immunity
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of JAK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for JAK3 purchase    MBS900324 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing JAK3. The ELISA analytical biochemical technique of the MBS900324 kit is based on JAK3 antibody-JAK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect JAK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, JAK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for JAK3 elisa kit

   For samples: For the quantitative determination of mouse tyrosine-protein kinase JAK3 (JAK3) concentrations in serum, plasma, tissue homogenates, cell lysates.

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for JAK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any JAK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for JAK3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of JAK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of JAK3 elisa kit    JAK3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS900324. Click to request current manual
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NCBI/Uniprot data below describe general gene information for JAK3. It may not necessarily be applicable to this product.
 NCBI GI #    300192999
 NCBI GeneID    16453
 NCBI Accession #    NP_001177759.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001190830.1 [Other Products]
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 UniProt Primary Accession #    Q62137 [Other Products]
 UniProt Secondary Accession #    Q0D2M8; Q61746; Q61747; Q8BTY6; Q8BYU2; A2RRI3 [Other Products]
 UniProt Related Accession #    Q62137 [Other Products]
 Molecular Weight    122,641 Da
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 NCBI Official Full Name    tyrosine-protein kinase JAK3
 NCBI Official Synonym Full Names    Janus kinase 3
 NCBI Official Symbol    Jak3 [Similar Products]
 NCBI Official Synonym Symbols   
fae; wil
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase JAK3
 UniProt Protein Name    Tyrosine-protein kinase JAK3
 UniProt Synonym Protein Names   
Janus kinase 3; JAK-3
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    Jak3 [Similar Products]
 UniProt Synonym Gene Names    JAK-3 [Similar Products]
 UniProt Entry Name    JAK3_MOUSE
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 UniProt Comments for JAK3    JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; JakA family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; cytoplasm; intracellular; cytosol

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; protein phosphatase binding; ATP binding; protein kinase activity; hormone receptor binding

Biological Process: positive regulation of immune response; peptidyl-tyrosine phosphorylation; negative regulation of interleukin-10 production; negative regulation of FasL biosynthetic process; negative regulation of T-helper 1 cell differentiation; protein amino acid autophosphorylation; positive regulation of activated T cell proliferation; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; elevation of cytosolic calcium ion concentration; erythrocyte differentiation; T cell homeostasis; positive regulation of T cell proliferation; inflammatory response; negative regulation of interleukin-12 production; cell migration; immune system process; cytokine and chemokine mediated signaling pathway; lymph node development; tyrosine phosphorylation of Stat5 protein; negative regulation of dendritic cell cytokine production; tyrosine phosphorylation of STAT protein; B cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of calcium ion transport; negative regulation of T cell activation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway
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 Research Articles on JAK3    1. JAK3 deficiency leads to intestinal sodium (Na+) loss, decrease of blood pressure, increased aldosterone release and subsequent stimulation of renal tubular Na(+) reabsorption.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with JAK3 elisa kitDiseases associated with JAK3 elisa kit
 Products by Pathway  Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
 IL-4 Signaling Pathway antibodies  IL-4 Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Lymphoma Antibodies  >103 publications with JAK3 and Lymphoma
 Inflammation Antibodies  >94 publications with JAK3 and Inflammation
 Arthritis, Rheumatoid Antibodies  >53 publications with JAK3 and Arthritis, Rheumatoid
 Necrosis Antibodies  >43 publications with JAK3 and Necrosis
 Breast Neoplasms Antibodies  >18 publications with JAK3 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >17 publications with JAK3 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >16 publications with JAK3 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >15 publications with JAK3 and Kidney Diseases
 Liver Diseases Antibodies  >10 publications with JAK3 and Liver Diseases
 Neoplasm Invasiveness Antibodies  >9 publications with JAK3 and Neoplasm Invasiveness
Organs/Tissues associated with JAK3 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Thymus Antibodies  >31 publications with JAK3 and Thymus
 Intestine Antibodies  >11 publications with JAK3 and Intestine
 Connective Tissue Antibodies  >9 publications with JAK3 and Connective Tissue
 Testis Antibodies  >5 publications with JAK3 and Testis
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