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KLK10 elisa kit :: Mouse Kallikrein 10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722747
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $470 +1 FREE 8GB USB
96-Strip-Wells  /  $675 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Kallikrein 10 (KLK10), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Kallikrein 10 ELISA Kit

 Product Gene Name   

KLK10 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602673
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of KLK10. No significant cross-reactivity or interference between KLK10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between KLK10 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
 Assay Type    Quantitative Sandwich
 Sensitivity    0.1 ng/mL.
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KLK10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KLK10 purchase    MBS722747 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kallikrein 10 (KLK10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLK10, with an estimated sensitivity of 0.1 ng/mL. The ELISA analytical biochemical technique of the MBS722747 kit is based on KLK10 antibody-KLK10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLK10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLK10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for KLK10 elisa kit

   Intended Uses: This KLK10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse KLK10. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: KLK10 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for KLK10. Standards or samples are then added to the microtiter plate wells and KLK10 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of KLK10 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for KLK10 are added to each well to "sandwich" the KLK10 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain KLK10 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK10 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for KLK10 elisa kit    Signal Transduction
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 Typical Standard Curve/Testing Data of KLK10 elisa kit    KLK10 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS722747. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KLK10. It may not necessarily be applicable to this product.
 NCBI GI #    117422433
 NCBI GeneID    5655
 NCBI Accession #    NP_001070968.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001077500.1 [Other Products]
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 UniProt Secondary Accession #    Q53YL3; Q99920; Q9GZW9; A6NC12 [Other Products]
 UniProt Related Accession #    O43240 [Other Products]
 Molecular Weight    30,170 Da
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 NCBI Official Full Name    kallikrein-10 preproprotein
 NCBI Official Synonym Full Names    kallikrein-related peptidase 10
 NCBI Official Symbol    KLK10 [Similar Products]
 NCBI Official Synonym Symbols   
NES1; PRSSL1
[Similar Products]
 NCBI Protein Information    kallikrein-10; protease serine-like 1; kallikrein 10 protein 1; kallikrein 10 protein 2; kallikrein 10 protein 3; kallikrein 10 protein 4; kallikrein 10 protein 7; kallikrein 10 protein 8; kallikrein 10 protein 9; kallikrein 10 protein 12; protease, serine-like, 1; normal epithelial cell-specific 1; breast normal epithelial cell associated serine protease
 UniProt Protein Name    Kallikrein-10
 UniProt Synonym Protein Names   
Normal epithelial cell-specific 1; Protease serine-like 1
 Protein Family    Kallikrein
 UniProt Gene Name    KLK10 [Similar Products]
 UniProt Synonym Gene Names    NES1; PRSSL1 [Similar Products]
 UniProt Entry Name    KLK10_HUMAN
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 NCBI Summary for KLK10    Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
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 UniProt Comments for KLK10    KLK10: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Tumor suppressor; Protease; Secreted, signal peptide; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: cell cycle; proteolysis
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 Research Articles on KLK10    1. KLK10 expression is an independent biomarker of unfavorable prognosis in patients with gastric cancer.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KLK10 elisa kitDiseases associated with KLK10 elisa kit
 Reference Product  PubMed Publications
 KLK5 elisa kit  >16 publications with KLK10 and KLK5
 KLK7 elisa kit  >14 publications with KLK10 and KLK7
 KLK8 elisa kit  >14 publications with KLK10 and KLK8
 KLK11 elisa kit  >10 publications with KLK10 and KLK11
 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >27 publications with KLK10 and Ovarian Neoplasms
 Breast Neoplasms Antibodies  >27 publications with KLK10 and Breast Neoplasms
 Lung Neoplasms Antibodies  >7 publications with KLK10 and Lung Neoplasms
 Respiratory Tract Diseases Antibodies  >6 publications with KLK10 and Respiratory Tract Diseases
 Stomach Neoplasms Antibodies  >6 publications with KLK10 and Stomach Neoplasms
 Prostatic Neoplasms Antibodies  >6 publications with KLK10 and Prostatic Neoplasms
 Carcinoma, Squamous Cell Antibodies  >6 publications with KLK10 and Carcinoma, Squamous Cell
 Neoplasms, Experimental Antibodies  >5 publications with KLK10 and Neoplasms, Experimental
 Immune System Diseases Antibodies  >3 publications with KLK10 and Immune System Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with KLK10 and Cell Transformation, Neoplastic
Organs/Tissues associated with KLK10 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >24 publications with KLK10 and Prostate
 Lung Antibodies  >7 publications with KLK10 and Lung
 Testis Antibodies  >6 publications with KLK10 and Testis
 Ovary Antibodies  >5 publications with KLK10 and Ovary
 Skin Antibodies  >5 publications with KLK10 and Skin
 Ascites Antibodies  >3 publications with KLK10 and Ascites
 Mouth Antibodies  >3 publications with KLK10 and Mouth
 Brain Antibodies  >3 publications with KLK10 and Brain
 Pancreas Antibodies  >3 publications with KLK10 and Pancreas
 Thyroid Antibodies  >2 publications with KLK10 and Thyroid
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