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KLK3 elisa kit :: Mouse Kallikrein 3 (KLK3) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS453842
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $505 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,275 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,420 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Kallikrein 3 (KLK3), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Kallikrein 3 (KLK3) ELISA Kit

 Product Synonym Names    CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase
 Product Gene Name   

KLK3 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P26262
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of KLK3. No significant cross-reactivity or interference between KLK3 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids.
 Assay Type    Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    0.058ng/mL
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLK3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLK3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KLK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KLK3 purchase    MBS453842 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kallikrein 3 (KLK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing KLK3, with an estimated sensitivity of 0.058ng/mL. The ELISA analytical biochemical technique of the MBS453842 kit is based on KLK3 antibody-KLK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KLK3 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK3 in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KLK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KLK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of KLK3 elisa kit    KLK3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS453842. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KLK3. It may not necessarily be applicable to this product.
 NCBI GI #    236465805
 NCBI GeneID    16621
 NCBI Accession #    NP_032481.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008455.3 [Other Products]
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 UniProt Primary Accession #    P26262 [Other Products]
 UniProt Secondary Accession #    Q8R0P5 [Other Products]
 UniProt Related Accession #    P26262 [Other Products]
 Molecular Weight    71,383 Da
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 NCBI Official Full Name    plasma kallikrein preproprotein
 NCBI Official Synonym Full Names    kallikrein B, plasma 1
 NCBI Official Symbol    Klkb1 [Similar Products]
 NCBI Official Synonym Symbols   
APS; PSA; Kal3; Klk3; Kal-3
[Similar Products]
 NCBI Protein Information    plasma kallikrein
 UniProt Protein Name    Plasma kallikrein
 UniProt Synonym Protein Names   
Fletcher factor; Kininogenin; Plasma prekallikrein
 Protein Family    Glandular kallikrein
 UniProt Gene Name    Klkb1 [Similar Products]
 UniProt Synonym Gene Names    Klk3; Pk [Similar Products]
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 NCBI Summary for KLK3    This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric enzyme comprised of heavy and light chains. A complete deletion of the encoded protein prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. [provided by RefSeq, May 2016]
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 UniProt Comments for KLK3    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
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 Research Articles on KLK3    1. Klkb1(-/-) mice have a novel mechanism for thrombosis protection in addition to reduced contact activation. This pathway arises when bradykinin delivery to vasculature is compromised and mediated by increased receptor Mas, prostacyclin, Sirt1, and KLF4
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KLK3 elisa kitPathways associated with KLK3 elisa kit
 Reference Product  PubMed Publications
 F11 elisa kit  >8 publications with KLK3 and F11
 F12 elisa kit  >7 publications with KLK3 and F12
 Kng1 elisa kit  >2 publications with KLK3 and Kng1
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway Of Fibrin Clot Formation antibodies  Intrinsic Pathway Of Fibrin Clot Formation Diagram
Diseases associated with KLK3 elisa kitOrgans/Tissues associated with KLK3 elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >15 publications with KLK3 and Cardiovascular Diseases
 Thrombosis Antibodies  >8 publications with KLK3 and Thrombosis
 Hypertension Antibodies  >6 publications with KLK3 and Hypertension
 Kidney Diseases Antibodies  >3 publications with KLK3 and Kidney Diseases
 Inflammation Antibodies  >3 publications with KLK3 and Inflammation
 Edema Antibodies  >2 publications with KLK3 and Edema
 Mental Disorders Antibodies  >2 publications with KLK3 and Mental Disorders
 Lung Neoplasms Antibodies  >2 publications with KLK3 and Lung Neoplasms
 Pulmonary Embolism Antibodies  >2 publications with KLK3 and Pulmonary Embolism
 Heart Diseases Antibodies  >1 publications with KLK3 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >7 publications with KLK3 and Kidney
 Liver Antibodies  >2 publications with KLK3 and Liver
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