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KISS1 elisa kit :: Mouse KiSS-1 metastasis-suppressor ELISA Kit

Scan QR to view Datasheet Catalog #    MBS916129
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 
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 Product Name   

KiSS-1 metastasis-suppressor (KISS1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Metastasis-suppressor KiSS-1, KISS1 ELISA Kit

 Product Synonym Names    Mouse Metastasis-suppressor KiSS-1 (KISS1) ELISA kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor
 Product Gene Name   

KISS1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS916129 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for Q6Y4S4
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of mouse KISS1. No significant cross-reactivity or interference between mouse KISS1 and analogues was observed.
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 Samples    Serum, plasma, cell culture supernates, tissue homogenates
 Assay Type    Sandwich
 Detection Range    12.5 pg/ml-800 pg/ml.
 Sensitivity    The minimum detectable dose of mouse KISS1 is typically less than 3.125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
 Inter-assay Precision    Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KISS1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KISS1 purchase    MBS916129 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the KiSS-1 metastasis-suppressor (KISS1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 12.5 pg/ml-800 pg/ml in biological research samples containing KISS1, with an estimated sensitivity of The minimum detectable dose of mouse KISS1 is typically less than 3.125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. The ELISA analytical biochemical technique of the MBS916129 kit is based on KISS1 antibody-KISS1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KISS1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KISS1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, cell culture supernates, tissue homogenates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KISS1 elisa kit

   Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KISS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KISS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of KISS1 elisa kit    KISS1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS916129. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KISS1. It may not necessarily be applicable to this product.
 NCBI GI #    144226215
 NCBI GeneID    280287
 NCBI Accession #    NP_839991.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_178260.3 [Other Products]
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 UniProt Primary Accession #    Q6Y4S4 [Other Products]
 UniProt Secondary Accession #    Q149Z8; Q68Y93; Q80XF6 [Other Products]
 UniProt Related Accession #    Q6Y4S4 [Other Products]
 Molecular Weight    14,117 Da
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 NCBI Official Full Name    metastasis-suppressor KiSS-1
 NCBI Official Synonym Full Names    KiSS-1 metastasis-suppressor
 NCBI Official Symbol    Kiss1 [Similar Products]
 NCBI Official Synonym Symbols   
metastatin
[Similar Products]
 NCBI Protein Information    metastasis-suppressor KiSS-1; metastin; kisspeptin-1; 0610012C01Rik; Kiss1 metastasis-suppressor
 UniProt Protein Name    Metastasis-suppressor KiSS-1
 UniProt Synonym Protein Names   
Kisspeptin-1Cleaved into the following 2 chains:MetastinAlternative name(s):Kisspeptin-52
 Protein Family    Metastasis-suppressor
 UniProt Gene Name    Kiss1 [Similar Products]
 UniProt Entry Name    KISS1_MOUSE
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 UniProt Comments for KISS1    KiSS-1: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Belongs to the KISS1 family.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; neuron projection; cell soma; apical plasma membrane; cytoplasm; cytosol

Molecular Function: protein binding; G-protein-coupled receptor binding; kisspeptin receptor binding

Biological Process: positive regulation of growth hormone secretion; generation of ovulation cycle rhythm; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; negative regulation of cell migration; positive regulation of synaptic transmission; positive regulation of luteinizing hormone secretion
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 Research Articles on KISS1    1. Sex differences in reproductive genes Kiss1, Gnrh1, and Gad1 are critically disturbed in GABAB1-deficient mice, probably altering the organization and development of neural circuits governing the reproductive axis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with KISS1 elisa kitDiseases associated with KISS1 elisa kit
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >123 publications with KISS1 and Neoplasms
 Gonadal Disorders Antibodies  >70 publications with KISS1 and Gonadal Disorders
 Neoplasm Metastasis Antibodies  >67 publications with KISS1 and Neoplasm Metastasis
 Carcinoma Antibodies  >44 publications with KISS1 and Carcinoma
 Breast Neoplasms Antibodies  >32 publications with KISS1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >22 publications with KISS1 and Adenocarcinoma
 Puberty, Delayed Antibodies  >21 publications with KISS1 and Puberty, Delayed
 Infertility, Female Antibodies  >19 publications with KISS1 and Infertility, Female
 Infertility, Male Antibodies  >16 publications with KISS1 and Infertility, Male
 Nervous System Diseases Antibodies  >13 publications with KISS1 and Nervous System Diseases
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