Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow DCT rightarrow Mouse DCT  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Kidney Injury Molecule 1 (Kim1) ELISA Kit
 • Tyrosine 3-Tryptophan 5 Monooxygenase Activation Protein The... ELISA Kit
 • interleukin 1 family, member 8 (eta) (IL1F8) ELISA Kit
 • Histone Deacetylase 1 (HDAC1) ELISA Kit
 • ACTN3 ELISA Kit
 • Mouse Serum pool Serum
 • Sm (Smith) Antigen Antibody
 • IL-9 Active Protein
 • DGKK Antibody
 • MOBKL2A-B Antibody
 • FGF acidic Recombinant Protein
 • myeloid differentiation primary response gene (88) (MYD88) ELISA Kit
 • KCNJ8 Antibody
 • CEP152 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

DCT elisa kit :: Mouse L-dopachrome tautomerase (DCT) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7245680
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

L-dopachrome tautomerase (DCT), ELISA Kit

★Popular Item★
 Also Known As   

Mouse L-dopachrome tautomerase (DCT) ELISA Kit

 Product Gene Name   

DCT elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    191275
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of DCT. No significant cross-reactivity or interference between DCT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DCT and all the analogues, therefore, cross reaction may still exist in some cases.
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Sensitivity    1.0 ng/mL.
 Intended Uses    This DCT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse DCT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of DCT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for DCT purchase    MBS7245680 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the L-dopachrome tautomerase (DCT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DCT, with an estimated sensitivity of 1.0 ng/mL. The ELISA analytical biochemical technique of the MBS7245680 kit is based on DCT antibody-DCT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DCT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DCT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for DCT elisa kit

   Principle of the assay: DCT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DCT antibody and an DCT-HRP conjugate. The assay sample and buffer are incubated together with DCT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCT concentration since DCT from samples and DCT-HRP conjugate compete for the anti-DCT antibody binding site. Since the number of sites is limited, as more sites are occupied by DCT from the sample, fewer sites are left to bind DCT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCT concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for DCT elisa kit    Signal Transduction
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of DCT elisa kit    DCT elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS7245680. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for DCT. It may not necessarily be applicable to this product.
 NCBI GI #    6041667
 NCBI GeneID    1638
 NCBI Accession #    NP_001913.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001922.3 [Other Products]
Section Bar
 UniProt Secondary Accession #    Q09GT4 [Other Products]
 UniProt Related Accession #    P40126 [Other Products]
 Molecular Weight    62,929 Da
Table BarTOPTable Bar
 NCBI Official Full Name    L-dopachrome tautomerase isoform 1
 NCBI Official Synonym Full Names    dopachrome tautomerase
 NCBI Official Symbol    DCT [Similar Products]
 NCBI Official Synonym Symbols   
TRP-2; TYRP2
[Similar Products]
 NCBI Protein Information    L-dopachrome tautomerase; DT; TRP2; dopachrome delta-isomerase; tyrosine-related protein 2; L-dopachrome Delta-isomerase; tyrosinase-related protein 2; dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
 UniProt Protein Name    L-dopachrome tautomerase
 UniProt Synonym Protein Names   
L-dopachrome Delta-isomerase; Tyrosinase-related protein 2; TRP-2; TRP2
 Protein Family    L-dopachrome tautomerase
 UniProt Gene Name    DCT [Similar Products]
 UniProt Synonym Gene Names    TYRP2; DCT; DT; TRP-2; TRP2 [Similar Products]
 UniProt Entry Name    TYRP2_HUMAN
Section Bar
 UniProt Comments for DCT    DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tyrosine; Cell development/differentiation; Isomerase; Membrane protein, integral; EC 5.3.3.12

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: melanosome membrane; integral to membrane; melanosome; cytosol

Molecular Function: copper ion binding; oxidoreductase activity; dopachrome isomerase activity

Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development
Table BarTOPTable Bar
 Research Articles on DCT    1. The results suggested that the miRNAs may be involved in MITF regulation of TYR, TYRP1 and TYRP2, which provides a new clue for understanding the role of miRNAs in melanocyte dysfunctional disease.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with DCT elisa kitPathways associated with DCT elisa kit
 Reference Product  PubMed Publications
 TYRP1 elisa kit  >66 publications with DCT and TYRP1
 MITF elisa kit  >54 publications with DCT and MITF
 SILV elisa kit  >4 publications with DCT and SILV
 MLANA elisa kit  >2 publications with DCT and MLANA
 OTX2 elisa kit  >2 publications with DCT and OTX2
 SOX9 elisa kit  >1 publications with DCT and SOX9
 Products by Pathway  Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Melanogenesis Pathway antibodies  Melanogenesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Diseases associated with DCT elisa kitOrgans/Tissues associated with DCT elisa kit
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >54 publications with DCT and Kidney Diseases
 Carcinoma Antibodies  >46 publications with DCT and Carcinoma
 Heart Diseases Antibodies  >39 publications with DCT and Heart Diseases
 Neoplasms, Experimental Antibodies  >35 publications with DCT and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >23 publications with DCT and Adenocarcinoma
 Anemia Antibodies  >17 publications with DCT and Anemia
 Necrosis Antibodies  >17 publications with DCT and Necrosis
 Lung Neoplasms Antibodies  >16 publications with DCT and Lung Neoplasms
 Hypertrophy Antibodies  >14 publications with DCT and Hypertrophy
 Heart Failure Antibodies  >13 publications with DCT and Heart Failure
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >278 publications with DCT and Kidney
 Eye Antibodies  >156 publications with DCT and Eye
 Skin Antibodies  >85 publications with DCT and Skin
 Heart Antibodies  >67 publications with DCT and Heart
 Brain Antibodies  >63 publications with DCT and Brain
 Bone Antibodies  >43 publications with DCT and Bone
 Lung Antibodies  >34 publications with DCT and Lung
 Embryonic Tissue Antibodies  >23 publications with DCT and Embryonic Tissue
 Intestine Antibodies  >15 publications with DCT and Intestine
 Connective Tissue Antibodies  >13 publications with DCT and Connective Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions