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Lep elisa kit :: Mouse Leptin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2885529
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $325 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,290 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Leptin, ELISA Kit

★Popular Item★
 Also Known As   

Mouse Leptin ELISA Kit

 Product Synonym Names    Leptin; Obesity factor; Lep; Ob
 Product Gene Name   

Lep elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2885529 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for P41160
 Species Reactivity    Mouse
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 Specificity    Recombinant and natural Mouse Leptin
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 Samples    Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
 Detection Range    0.312-20 ng/mL
 Intra-assay Precision    Intra-Assay CV: <=5.7%
 Inter-assay Precision    Inter-Assay CV: <=7.9%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Lep elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lep purchase    MBS2885529 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leptin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20 ng/mL in biological research samples containing Lep. The ELISA analytical biochemical technique of the MBS2885529 kit is based on Lep antibody-Lep antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lep antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lep. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Lep elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of Lep elisa kit    Lep elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2885529. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Lep. It may not necessarily be applicable to this product.
 NCBI GI #    6678678
 NCBI GeneID    16846
 NCBI Accession #    NP_032519.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008493.3 [Other Products]
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 UniProt Primary Accession #    P41160 [Other Products]
 UniProt Related Accession #    P41160 [Other Products]
 Molecular Weight    18,709 Da
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 NCBI Official Full Name    leptin
 NCBI Official Synonym Full Names    leptin
 NCBI Official Symbol    Lep [Similar Products]
 NCBI Official Synonym Symbols   
ob; obese
[Similar Products]
 NCBI Protein Information    leptin
 UniProt Protein Name    Leptin
 UniProt Synonym Protein Names   
Obesity factor
 Protein Family    Leptin
 UniProt Gene Name    Lep [Similar Products]
 UniProt Synonym Gene Names    Ob [Similar Products]
 UniProt Entry Name    LEP_MOUSE
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 UniProt Comments for Lep    leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted, signal peptide; Secreted; Hormone; Cell development/differentiation

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding; receptor binding

Biological Process: adult feeding behavior; bile acid metabolic process; central nervous system neuron development; cholesterol metabolic process; eating behavior; energy reserve metabolic process; fatty acid beta-oxidation; fatty acid catabolic process; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process; hormone metabolic process; insulin secretion; intestinal absorption; leptin-mediated signaling pathway; leukocyte tethering or rolling; lipid metabolic process; negative regulation of apoptosis; negative regulation of appetite; negative regulation of autophagy; negative regulation of glucose import; negative regulation of metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of vasoconstriction; ovulation from ovarian follicle; placenta development; positive regulation of cell proliferation; positive regulation of developmental growth; positive regulation of insulin receptor signaling pathway; positive regulation of ion transport; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of myeloid cell differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of TOR signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; prostaglandin secretion; regulation of angiogenesis; regulation of blood pressure; regulation of bone remodeling; regulation of cell cycle; regulation of cholesterol absorption; regulation of endothelial cell proliferation; regulation of fat cell differentiation; regulation of gluconeogenesis; regulation of insulin secretion; regulation of metabolic process; regulation of natural killer cell activation; regulation of natural killer cell mediated cytotoxicity; regulation of natural killer cell proliferation; regulation of nitric-oxide synthase activity; regulation of protein amino acid phosphorylation; regulation of steroid biosynthetic process; response to dietary excess; response to insulin stimulus; sexual reproduction; signal transduction; T cell differentiation; tyrosine phosphorylation of STAT protein
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 Research Articles on Lep    1. These findings demonstrate that hyperleptinemic DIO mice retain leptin suppression of feeding comparable to lean mice
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lep elisa kitPathways associated with Lep elisa kit
 Reference Product  PubMed Publications
 Lepr elisa kit  >193 publications with Lep and Lepr
 Stat3 elisa kit  >18 publications with Lep and Stat3
 Ghrl elisa kit  >13 publications with Lep and Ghrl
 Il6 elisa kit  >12 publications with Lep and Il6
 Irs1 elisa kit  >7 publications with Lep and Irs1
 Jak2 elisa kit  >6 publications with Lep and Jak2
 Socs3 elisa kit  >1 publications with Lep and Socs3
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Incretin Synthesis, Secretion, And Inactivation Pathway antibodies  Incretin Synthesis, Secretion, And Inactivation Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
Diseases associated with Lep elisa kitOrgans/Tissues associated with Lep elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >392 publications with Lep and Obesity
 Pain Antibodies  >195 publications with Lep and Pain
 Insulin Resistance Antibodies  >125 publications with Lep and Insulin Resistance
 Hyperinsulinism Antibodies  >84 publications with Lep and Hyperinsulinism
 Weight Gain Antibodies  >66 publications with Lep and Weight Gain
 Weight Loss Antibodies  >64 publications with Lep and Weight Loss
 Cardiovascular Diseases Antibodies  >58 publications with Lep and Cardiovascular Diseases
 Inflammation Antibodies  >57 publications with Lep and Inflammation
 Fatty Liver Antibodies  >48 publications with Lep and Fatty Liver
 Liver Diseases Antibodies  >45 publications with Lep and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >237 publications with Lep and Brain
 Adipose Tissue Antibodies  >182 publications with Lep and Adipose Tissue
 Skin Antibodies  >117 publications with Lep and Skin
 Bone Marrow Antibodies  >12 publications with Lep and Bone Marrow
 Salivary Gland Antibodies  >5 publications with Lep and Salivary Gland
 Bladder Antibodies  >5 publications with Lep and Bladder
 Thymus Antibodies  >4 publications with Lep and Thymus
 Mammary Gland Antibodies  >4 publications with Lep and Mammary Gland
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