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LEI elisa kit :: Mouse Leukocyte Elastase Inhibitor (LEI) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2023550 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
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 Product Name   

Leukocyte Elastase Inhibitor (LEI), ELISA Kit

★Popular Item★
 Also Known As   

Leukocyte Elastase Inhibitor (LEI) ELISA Kit

 Product Synonym Names    SERPINB1; EI; ELANH2; LEI; M/NEI; MNEI; PI2; Serpin Peptidase Inhibitor Clade B Member 1; Anti-Elastase; Monocyte/Neutrophil Elastase Inhibitor
 Product Gene Name   

LEI elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    130135
 3D Structure    ModBase 3D Structure for P30740
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Leukocyte Elastase Inhibitor (LEI).
No significant cross-reactivity or interference between Leukocyte Elastase Inhibitor (LEI) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma and Other Biological Fluids
 Detection Range    15.62-1000pg/mL
 Sensitivity    < 6.4pg/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Leukocyte Elastase Inhibitor (LEI) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LEI elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LEI purchase    MBS2023550 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Leukocyte Elastase Inhibitor (LEI) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.62-1000pg/mL in biological research samples containing LEI, with an estimated sensitivity of < 6.4pg/mL. The ELISA analytical biochemical technique of the MBS2023550 kit is based on LEI antibody-LEI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LEI antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LEI. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LEI elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Elastase Inhibitor (LEI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Elastase Inhibitor (LEI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Elastase Inhibitor (LEI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukocyte Elastase Inhibitor (LEI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LEI elisa kit    Infection immunity
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 Typical Testing Data/Standard Curve (for reference only) of LEI elisa kit    LEI elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2023550. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LEI. It may not necessarily be applicable to this product.
 NCBI GI #    13489087
 NCBI GeneID    1992
 NCBI Accession #    NP_109591.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_030666.3 [Other Products]
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 UniProt Primary Accession #    P30740 [Other Products]
 UniProt Secondary Accession #    Q53FB9; Q5W0E1; Q9UDF8; A8K5L2; B4DNT0 [Other Products]
 UniProt Related Accession #    P30740 [Other Products]
 Molecular Weight    26,073 Da
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 NCBI Official Full Name    leukocyte elastase inhibitor
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade B (ovalbumin), member 1
 NCBI Official Symbol    SERPINB1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    leukocyte elastase inhibitor
 UniProt Protein Name    Leukocyte elastase inhibitor
 UniProt Synonym Protein Names   
Monocyte/neutrophil elastase inhibitor; EI; M/NEI; Peptidase inhibitor 2; PI-2; Serpin B1
 Protein Family    Leukocyte elastase inhibitor
 UniProt Gene Name    SERPINB1 [Similar Products]
 UniProt Synonym Gene Names    ELANH2; MNEI; PI2; LEI; EI; M/NEI; PI-2 [Similar Products]
 UniProt Entry Name    ILEU_HUMAN
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 NCBI Summary for LEI    The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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 UniProt Comments for LEI    SERPINB1: Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: extracellular space; membrane; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity
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 Research Articles on LEI    1. Decreased expression of SERPINB1 correlates with tumor invasion and poor prognosis in hepatocellular carcinoma.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LEI elisa kitPathways associated with LEI elisa kit
 Reference Product  PubMed Publications
 CTSG elisa kit  >5 publications with LEI and CTSG
 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with LEI elisa kitOrgans/Tissues associated with LEI elisa kit
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >17 publications with LEI and Lung Diseases
 Inflammation Antibodies  >13 publications with LEI and Inflammation
 Lung Neoplasms Antibodies  >3 publications with LEI and Lung Neoplasms
 Neoplasm Invasiveness Antibodies  >2 publications with LEI and Neoplasm Invasiveness
 Breast Neoplasms Antibodies  >2 publications with LEI and Breast Neoplasms
 Adenocarcinoma Antibodies  >2 publications with LEI and Adenocarcinoma
 Glioblastoma Antibodies  >2 publications with LEI and Glioblastoma
 Liver Diseases Antibodies  >2 publications with LEI and Liver Diseases
 Carcinoma, Squamous Cell Antibodies  >2 publications with LEI and Carcinoma, Squamous Cell
 Brain Diseases Antibodies  >1 publications with LEI and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >27 publications with LEI and Blood
 Lung Antibodies  >20 publications with LEI and Lung
 Bone Marrow Antibodies  >4 publications with LEI and Bone Marrow
 Brain Antibodies  >4 publications with LEI and Brain
 Bone Antibodies  >4 publications with LEI and Bone
 Liver Antibodies  >3 publications with LEI and Liver
 Skin Antibodies  >2 publications with LEI and Skin
 Placenta Antibodies  >2 publications with LEI and Placenta
 Prostate Antibodies  >1 publications with LEI and Prostate
 Mouth Antibodies  >1 publications with LEI and Mouth
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