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LXRa elisa kit :: Mouse Liver X Receptor Alpha (LXRa) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020587 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Liver X Receptor Alpha (LXRa), ELISA Kit

 Also Known As   

Liver X Receptor Alpha (LXRa) ELISA Kit

 Product Synonym Names    NR1-H3; LXR-A; LXRA; RLD-1; Nuclear Receptor Subfamily 1,Group H,Member 3; Oxysterols receptor LXR-alpha
 Product Gene Name   

LXRa elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602423
 3D Structure    ModBase 3D Structure for Q13133
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Liver X Receptor Alpha (LXRa).
No significant cross-reactivity or interference between Liver X Receptor Alpha (LXRa) and analogues was observed.
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 Assay Type    Double-antibody Sandwich
 Samples    Tissue homogenates and Other Biological Fluids
 Detection Range    0.312-20ng/mL
 Sensitivity    < 0.103ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Liver X Receptor Alpha (LXRa) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
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 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of LXRa elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for LXRa purchase    MBS2020587 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Liver X Receptor Alpha (LXRa) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.312-20ng/mL in biological research samples containing LXRa, with an estimated sensitivity of < 0.103ng/mL. The ELISA analytical biochemical technique of the MBS2020587 kit is based on LXRa antibody-LXRa antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LXRa antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LXRa. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Tissue homogenates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for LXRa elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver X Receptor Alpha (LXRa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver X Receptor Alpha (LXRa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver X Receptor Alpha (LXRa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver X Receptor Alpha (LXRa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for LXRa elisa kit    Signal transduction; Metabolic pathway; Infection immunity; Hepatology
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 Typical Testing Data/Standard Curve (for reference only) of LXRa elisa kit    LXRa elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020587. Click to request current manual
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NCBI/Uniprot data below describe general gene information for LXRa. It may not necessarily be applicable to this product.
 NCBI GI #    216409704
 NCBI GeneID    10062
 NCBI Accession #    BAH02289.1 [Other Products]
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 UniProt Primary Accession #    Q13133 [Other Products]
 UniProt Secondary Accession #    Q8IW13; Q96H87; A8K3J9; D3DQR1 [Other Products]
 UniProt Related Accession #    Q13133 [Other Products]
 Molecular Weight    45,692 Da
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 NCBI Official Full Name    liver X receptor alpha
 NCBI Official Synonym Full Names    nuclear receptor subfamily 1, group H, member 3
 NCBI Official Symbol    NR1H3 [Similar Products]
 NCBI Official Synonym Symbols   
LXRA; LXR-a; RLD-1
[Similar Products]
 NCBI Protein Information    oxysterols receptor LXR-alpha
 UniProt Protein Name    Oxysterols receptor LXR-alpha
 UniProt Synonym Protein Names   
Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3
 UniProt Gene Name    NR1H3 [Similar Products]
 UniProt Synonym Gene Names    LXRA [Similar Products]
 UniProt Entry Name    NR1H3_HUMAN
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 NCBI Summary for LXRa    The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for LXRa    LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleus; receptor complex

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; sterol response element binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; cholesterol binding; steroid hormone receptor activity

Biological Process: negative regulation of lipid transport; transcription initiation from RNA polymerase II promoter; positive regulation of fatty acid biosynthetic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of toll-like receptor 4 signaling pathway; lipid homeostasis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of pinocytosis; positive regulation of cholesterol transport; regulation of circadian rhythm; cholesterol homeostasis; negative regulation of inflammatory response; positive regulation of lipoprotein lipase activity; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; response to progesterone stimulus; sterol homeostasis; negative regulation of macrophage activation; apoptotic cell clearance
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 Research Articles on LXRa    1. This study provides the first evidence to show LXR activation reduces cadmium-induced apoptotic cell death of human renal proximal tubular cells by inhibition of reactive oxygen species production and JNK activation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with LXRa elisa kitPathways associated with LXRa elisa kit
 Reference Product  PubMed Publications
 NR1H2 elisa kit  >42 publications with LXRa and NR1H2
 ABCA1 elisa kit  >18 publications with LXRa and ABCA1
 FASN elisa kit  >6 publications with LXRa and FASN
 CYP7A1 elisa kit  >3 publications with LXRa and CYP7A1
 REN elisa kit  >3 publications with LXRa and REN
 ABCG8 elisa kit  >2 publications with LXRa and ABCG8
 NR0B2 elisa kit  >2 publications with LXRa and NR0B2
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 Non-alcoholic Fatty Liver Disease (NAFLD) Pathway antibodies  Non-alcoholic Fatty Liver Disease (NAFLD) Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram
Diseases associated with LXRa elisa kitOrgans/Tissues associated with LXRa elisa kit
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >17 publications with LXRa and Fatty Liver
 Inflammation Antibodies  >12 publications with LXRa and Inflammation
 Liver Diseases Antibodies  >11 publications with LXRa and Liver Diseases
 Atherosclerosis Antibodies  >8 publications with LXRa and Atherosclerosis
 Cardiovascular Diseases Antibodies  >7 publications with LXRa and Cardiovascular Diseases
 Insulin Resistance Antibodies  >6 publications with LXRa and Insulin Resistance
 Hypercholesterolemia Antibodies  >5 publications with LXRa and Hypercholesterolemia
 Hypertriglyceridemia Antibodies  >4 publications with LXRa and Hypertriglyceridemia
 Dyslipidemias Antibodies  >3 publications with LXRa and Dyslipidemias
 Prenatal Exposure Delayed Effects Antibodies  >3 publications with LXRa and Prenatal Exposure Delayed Effects
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >74 publications with LXRa and Liver
 Blood Antibodies  >20 publications with LXRa and Blood
 Thyroid Antibodies  >8 publications with LXRa and Thyroid
 Brain Antibodies  >8 publications with LXRa and Brain
 Intestine Antibodies  >6 publications with LXRa and Intestine
 Heart Antibodies  >6 publications with LXRa and Heart
 Adipose Tissue Antibodies  >4 publications with LXRa and Adipose Tissue
 Mammary Gland Antibodies  >3 publications with LXRa and Mammary Gland
 Kidney Antibodies  >3 publications with LXRa and Kidney
 Pancreas Antibodies  >3 publications with LXRa and Pancreas
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