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Lta elisa kit :: Mouse Lymphotoxin-alpha ELISA Kit

Scan QR to view Datasheet Catalog #    MBS765171
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $295 +1 FREE 8GB USB
96-Strip-Wells  /  $390 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,015 +3 FREE 8GB USB
15x96-Strip-Wells  /  $4,285 +5 FREE 8GB USB
25x96-Strip-Wells  /  $6,715 +8 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Lymphotoxin-alpha (Lta), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Lymphotoxin-alpha ELISA Kit

 Product Synonym Names    Lta/tnfb/TNF-beta/TNFSF1/TNFSF1B/LT/LT-alpha/lymphotoxin alpha (TNF superfamily; member 1)/TNFBlymphotoxin-alpha/TNFSF1TNF-beta/tumor necrosis factor beta/Tumor necrosis factor ligand superfamily member 1
 Product Gene Name   

Lta elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS765171 COA    COA PDF
 OMIM    153440
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Lta. No significant crossreactivity or interference between Lta and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    15.6-1000pg/ml
 Sensitivity    <9.375pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lta were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lta were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Lta elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Lta purchase    MBS765171 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lymphotoxin-alpha (Lta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6-1000pg/ml in biological research samples containing Lta, with an estimated sensitivity of <9.375pg/ml. The ELISA analytical biochemical technique of the MBS765171 kit is based on Lta antibody-Lta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lta antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Lta. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Lta elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Lta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Lta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lta can be calculated.
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 Typical Standard Curve/Testing Data of Lta elisa kit    Lta elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS765171. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Lta. It may not necessarily be applicable to this product.
 NCBI GI #    229092381
 NCBI GeneID    4049
 NCBI Accession #    NP_001153212.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001159740.2 [Other Products]
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 UniProt Secondary Accession #    Q8N4C3; Q9UKS8 [Other Products]
 UniProt Related Accession #    P01374 [Other Products]
 Molecular Weight    22,297 Da
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 NCBI Official Full Name    lymphotoxin-alpha
 NCBI Official Synonym Full Names    lymphotoxin alpha
 NCBI Official Symbol    LTA [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    lymphotoxin-alpha
 UniProt Protein Name    Lymphotoxin-alpha
 UniProt Synonym Protein Names   
TNF-beta; Tumor necrosis factor ligand superfamily member 1
 Protein Family    Lymphotoxin
 UniProt Gene Name    LTA [Similar Products]
 UniProt Synonym Gene Names    TNFB; TNFSF1; LT-alpha [Similar Products]
 UniProt Entry Name    TNFB_HUMAN
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 NCBI Summary for Lta    The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
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 UniProt Comments for Lta    LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family.

Protein type: Secreted; Secreted, signal peptide; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; plasma membrane

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding

Biological Process: apoptosis; cell-cell signaling; defense response to Gram-positive bacterium; humoral immune response; lymph node development; negative regulation of fibroblast proliferation; positive regulation of apoptosis; positive regulation of chronic inflammatory response to antigenic stimulus; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of interferon-gamma production; response to drug; response to hypoxia; response to lipopolysaccharide; response to nutrient; signal transduction; tumor necrosis factor-mediated signaling pathway

Disease: Leprosy, Susceptibility To, 4; Myocardial Infarction, Susceptibility To; Psoriatic Arthritis, Susceptibility To
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 Research Articles on Lta    1. Studied six SNP loci: (rs2279115 of BCL2 gene, rs804270 of NEIL2 gene, rs909253 of LTA gene, rs2294008 of PSCA gene, rs3765524 and rs10509670 of PLCE1 gene) to evaluate gastric cancer risk using magnetic nanoparticles and universal tagged arrays.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Lta elisa kitPathways associated with Lta elisa kit
 Reference Product  PubMed Publications
 LTB elisa kit  >112 publications with Lta and LTB
 LGALS2 elisa kit  >19 publications with Lta and LGALS2
 CD40 elisa kit  >8 publications with Lta and CD40
 TNFRSF1A elisa kit  >8 publications with Lta and TNFRSF1A
 LTBR elisa kit  >7 publications with Lta and LTBR
 TNFRSF1B elisa kit  >5 publications with Lta and TNFRSF1B
 RIPK1 elisa kit  >3 publications with Lta and RIPK1
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 IL2 Signaling Events Mediated By STAT5 Pathway antibodies  IL2 Signaling Events Mediated By STAT5 Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
Diseases associated with Lta elisa kitOrgans/Tissues associated with Lta elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >405 publications with Lta and Necrosis
 Inflammation Antibodies  >372 publications with Lta and Inflammation
 Cardiovascular Diseases Antibodies  >245 publications with Lta and Cardiovascular Diseases
 Heart Diseases Antibodies  >155 publications with Lta and Heart Diseases
 Lung Diseases Antibodies  >113 publications with Lta and Lung Diseases
 Sepsis Antibodies  >110 publications with Lta and Sepsis
 Myocardial Infarction Antibodies  >88 publications with Lta and Myocardial Infarction
 Coronary Artery Disease Antibodies  >83 publications with Lta and Coronary Artery Disease
 Disease Models, Animal Antibodies  >72 publications with Lta and Disease Models, Animal
 Liver Diseases Antibodies  >49 publications with Lta and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >120 publications with Lta and Lung
 Heart Antibodies  >106 publications with Lta and Heart
 Spleen Antibodies  >30 publications with Lta and Spleen
 Lymph Antibodies  >21 publications with Lta and Lymph
 Pharynx Antibodies  >14 publications with Lta and Pharynx
 Thymus Antibodies  >11 publications with Lta and Thymus
 Tonsil Antibodies  >4 publications with Lta and Tonsil
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