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KARSL elisa kit :: Mouse Lysyl tRNA Synthetase ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2533484
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Lysyl tRNA Synthetase (KARSL), ELISA Kit

★Popular Item★
 Also Known As   

Mouse KARSL (Lysyl tRNA Synthetase) ELISA Kit

 Product Gene Name   

KARSL elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601421
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 Species Reactivity    Mouse
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 Specificity    This kit recognizes natural and recombinantMouseKARS. No significant cross-reactivity or interference between MouseKARS and analogues was observed.
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 Samples    Serum, plasma and other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    37.5pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of KARSL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for KARSL purchase    MBS2533484 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysyl tRNA Synthetase (KARSL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing KARSL, with an estimated sensitivity of 37.5pg/mL. The ELISA analytical biochemical technique of the MBS2533484 kit is based on KARSL antibody-KARSL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KARSL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KARSL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for KARSL elisa kit

   Intended Uses: This ELISA kit applies to the invitro quantitative determination of MouseKARSconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KARSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of KARS.You can calculate the concentration of KARSin the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of KARSL elisa kit    KARSL elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2533484. Click to request current manual
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NCBI/Uniprot data below describe general gene information for KARSL. It may not necessarily be applicable to this product.
 NCBI GI #    2366752
 NCBI GeneID    3735
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 UniProt Secondary Accession #    O14946; Q96J25; Q9HB23; A8MSK1; D3DUK4 [Other Products]
 UniProt Related Accession #    Q15046 [Other Products]
 Molecular Weight    71,497 Da
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 NCBI Official Full Name    Lysyl tRNA Synthetase
 NCBI Official Synonym Full Names    lysyl-tRNA synthetase
 NCBI Official Symbol    KARS [Similar Products]
 NCBI Official Synonym Symbols   
KRS; KARS1; KARS2; CMTRIB; DFNB89
[Similar Products]
 NCBI Protein Information    lysine--tRNA ligase
 UniProt Protein Name    Lysine--tRNA ligase
 UniProt Synonym Protein Names   
Lysyl-tRNA synthetase; LysRS
 UniProt Gene Name    KARS [Similar Products]
 UniProt Synonym Gene Names    KIAA0070; LysRS [Similar Products]
 UniProt Entry Name    SYK_HUMAN
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 NCBI Summary for KARSL    Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for KARSL    KARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. Homodimer; also part of a multisubunit complex that groups AIMP1, AIMP2, EEF1A1 and tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro. Interacts with AIMP2 (via N- terminus) and MITF. Interacts directly with HIV-1 virus GAG protein. Up-regulated by DARS and EEF1A1, but not by AIMP2. Belongs to the class-II aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 6.1.1.6; Translation; Ligase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; extracellular region; microtubule cytoskeleton; mitochondrial matrix; mitochondrion; nucleus; plasma membrane

Molecular Function: amino acid binding; ATP binding; lysine-tRNA ligase activity; metal ion binding; protein binding; tRNA binding

Biological Process: diadenosine tetraphosphate biosynthetic process; gene expression; lysyl-tRNA aminoacylation; selenium metabolic process; tRNA aminoacylation for protein translation; tRNA processing; viral reproduction

Disease: Charcot-marie-tooth Disease, Recessive Intermediate B; Deafness, Autosomal Recessive 89
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 Research Articles on KARSL    1. tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with KARSL elisa kitPathways associated with KARSL elisa kit
 Reference Product  PubMed Publications
 QARS elisa kit  >4 publications with KARSL and QARS
 LARS elisa kit  >3 publications with KARSL and LARS
 EPRS elisa kit  >3 publications with KARSL and EPRS
 MARS elisa kit  >2 publications with KARSL and MARS
 RARS elisa kit  >2 publications with KARSL and RARS
 AIMP1 elisa kit  >2 publications with KARSL and AIMP1
 AIMP2 elisa kit  >2 publications with KARSL and AIMP2
 DARS elisa kit  >1 publications with KARSL and DARS
 IARS elisa kit  >1 publications with KARSL and IARS
 Products by Pathway  Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis Pathway antibodies  Aminoacyl-tRNA Biosynthesis Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway antibodies  Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway Diagram
 Cytosolic TRNA Aminoacylation Pathway antibodies  Cytosolic TRNA Aminoacylation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Mitochondrial TRNA Aminoacylation Pathway antibodies  Mitochondrial TRNA Aminoacylation Pathway Diagram
 SeMet Incorporation Into Proteins Pathway antibodies  SeMet Incorporation Into Proteins Pathway Diagram
Diseases associated with KARSL elisa kitOrgans/Tissues associated with KARSL elisa kit
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >98 publications with KARSL and Cardiovascular Diseases
 Liver Diseases Antibodies  >30 publications with KARSL and Liver Diseases
 Inflammation Antibodies  >26 publications with KARSL and Inflammation
 Necrosis Antibodies  >21 publications with KARSL and Necrosis
 Kidney Diseases Antibodies  >19 publications with KARSL and Kidney Diseases
 Edema Antibodies  >18 publications with KARSL and Edema
 Hyperplasia Antibodies  >16 publications with KARSL and Hyperplasia
 Poisoning Antibodies  >15 publications with KARSL and Poisoning
 Adenocarcinoma Antibodies  >14 publications with KARSL and Adenocarcinoma
 Fibrosis Antibodies  >12 publications with KARSL and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >208 publications with KARSL and Blood
 Brain Antibodies  >159 publications with KARSL and Brain
 Heart Antibodies  >63 publications with KARSL and Heart
 Vascular Antibodies  >54 publications with KARSL and Vascular
 Liver Antibodies  >48 publications with KARSL and Liver
 Eye Antibodies  >47 publications with KARSL and Eye
 Lung Antibodies  >45 publications with KARSL and Lung
 Bone Antibodies  >45 publications with KARSL and Bone
 Nerve Antibodies  >39 publications with KARSL and Nerve
 Kidney Antibodies  >37 publications with KARSL and Kidney
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