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MIF elisa kit :: Mouse MIF ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2512304
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $410 +1 FREE 8GB USB
96-Strip-Wells  /  $490 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MIF, ELISA Kit

★Popular Item★
 Also Known As   

Mouse MIF (Macrophage Migration Inhibitory Factor) ELISA Kit

 Product Gene Name   

MIF elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    153620
 3D Structure    ModBase 3D Structure for P14174
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse MIF. No significant cross-reactivity or interference between Mouse MIF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse MIF and all the analogues, therefore, cross reaction may still exist.
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 Samples    Serum, Plasma, Biological Fluids
 Assay Type    Sandwich
 Detection Range    15.625-1000pg/mL
 Sensitivity    Min: 9.375pg/mL; Max: 1000pg/mL
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 Preparation and Storage    Store at 4 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MIF purchase    MBS2512304 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.625-1000pg/mL in biological research samples containing MIF, with an estimated sensitivity of Min: 9.375pg/mL; Max: 1000pg/mL. The ELISA analytical biochemical technique of the MBS2512304 kit is based on MIF antibody-MIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MIF elisa kit

   Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MIF in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse MIF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse MIF. You can calculate the concentration of Mouse MIF in the samples by comparing the OD of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MIF elisa kit    MIF elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2512304. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.
 NCBI GI #    47678571
 NCBI GeneID    4282
 NCBI Accession #    CAG30406.1 [Other Products]
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 UniProt Primary Accession #    P14174 [Other Products]
 UniProt Secondary Accession #    Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3 [Other Products]
 UniProt Related Accession #    P14174 [Other Products]
 Molecular Weight    12,476 Da
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 NCBI Official Full Name    MIF
 NCBI Official Synonym Full Names    macrophage migration inhibitory factor (glycosylation-inhibiting factor)
 NCBI Official Symbol    MIF [Similar Products]
 NCBI Official Synonym Symbols   
GIF; GLIF; MMIF
[Similar Products]
 NCBI Protein Information    macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase
 UniProt Protein Name    Macrophage migration inhibitory factor
 UniProt Synonym Protein Names   
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
 Protein Family    MIF-like protein
 UniProt Gene Name    MIF [Similar Products]
 UniProt Synonym Gene Names    GLIF; MMIF; MIF; GIF [Similar Products]
 UniProt Entry Name    MIF_HUMAN
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 NCBI Summary for MIF    This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MIF    MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Cell development/differentiation; Cytokine; EC 5.3.3.12; Amino Acid Metabolism - phenylalanine; Apoptosis; EC 5.3.2.1; Amino Acid Metabolism - tyrosine; Isomerase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle

Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; chemoattractant activity; receptor binding

Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; carboxylic acid metabolic process; positive regulation of lipopolysaccharide-mediated signaling pathway; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; positive regulation of MAP kinase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; negative regulation of mature B cell apoptosis; cell surface receptor linked signal transduction; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; positive regulation of cytokine secretion; negative regulation of apoptosis

Disease: Rheumatoid Arthritis, Systemic Juvenile
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 Research Articles on MIF    1. Both p53 wildtype and mutant human lung adenocarcinoma tumors rely on MIF family members for maximal cell growth and survival.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MIF elisa kitPathways associated with MIF elisa kit
 Reference Product  PubMed Publications
 CD74 elisa kit  >147 publications with MIF and CD74
 COPS5 elisa kit  >17 publications with MIF and COPS5
 MMP9 elisa kit  >13 publications with MIF and MMP9
 TAT elisa kit  >3 publications with MIF and TAT
 NME1 elisa kit  >2 publications with MIF and NME1
 UBC elisa kit  >2 publications with MIF and UBC
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Phenylalanine Metabolism Pathway antibodies  Phenylalanine Metabolism Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
Diseases associated with MIF elisa kitOrgans/Tissues associated with MIF elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >697 publications with MIF and Inflammation
 Necrosis Antibodies  >323 publications with MIF and Necrosis
 Disease Models, Animal Antibodies  >231 publications with MIF and Disease Models, Animal
 Lung Diseases Antibodies  >213 publications with MIF and Lung Diseases
 Carcinoma Antibodies  >162 publications with MIF and Carcinoma
 Sepsis Antibodies  >138 publications with MIF and Sepsis
 Arthritis, Rheumatoid Antibodies  >114 publications with MIF and Arthritis, Rheumatoid
 Heart Diseases Antibodies  >105 publications with MIF and Heart Diseases
 Pneumonia Antibodies  >83 publications with MIF and Pneumonia
 Kidney Diseases Antibodies  >81 publications with MIF and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1528 publications with MIF and Blood
 Brain Antibodies  >406 publications with MIF and Brain
 Vascular Antibodies  >244 publications with MIF and Vascular
 Skin Antibodies  >219 publications with MIF and Skin
 Lung Antibodies  >215 publications with MIF and Lung
 Muscle Antibodies  >201 publications with MIF and Muscle
 Liver Antibodies  >166 publications with MIF and Liver
 Spleen Antibodies  >164 publications with MIF and Spleen
 Heart Antibodies  >152 publications with MIF and Heart
 Lymph Antibodies  >130 publications with MIF and Lymph
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