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CXCL2 elisa kit :: Mouse MIP-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175915
 Typical Testing Data/Standard Curve
Unit / Price
96-Strip-Wells  /  $475 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   


 Also Known As   

Mouse MIP-2 PicoKine ELISA Kit

 Product Synonym Names    C-X-C motif chemokine 2; chemokine (C-X-C motif) ligand 2; C-X-C motif chemokine 2; Chemokine (C-X-C motif) ligand 2; CINC-2a; Cxcl2; CXCL2_HUMAN; Gro beta; Gro-beta; GRO-beta(5-73); GRO-beta-T; GRO2; GRO2 oncogene; GROb; Growth-regulated protein beta; Hematopoietic synergistic factor; HSF; Macrophage inflammatory protein 2-alpha; Melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MIP 2; MIP 2a; MIP2; MIP2-alpha; MIP2A; SB-251353; SCYB2
 Product Gene Name   

CXCL2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P10889
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse MIP-2
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    15.6pg/ml-1000pg/ml
 Sensitivity    < 5pg/ml
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of CXCL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for CXCL2 purchase    MBS175915 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIP-2 (CXCL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6pg/ml-1000pg/ml in biological research samples containing CXCL2, with an estimated sensitivity of < 5pg/ml. The ELISA analytical biochemical technique of the MBS175915 kit is based on CXCL2 antibody-CXCL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CXCL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CXCL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for CXCL2 elisa kit

   Principle of the assay: mouse MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MIP-2 has been precoated onto 96-well plates. Standards(E.coli, A28-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MIP-2 amount of sample captured in plate.
Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells. The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8KDa
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  Typical Testing Data/Standard Curve of CXCL2 elisa kit    Mouse MIP-2 PicoKine ELISA Kit standard curve
CXCL2 elisa kit  Typical Testing Data/Standard Curve image
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Sample Manual Insert of MBS175915. Click to request current manual
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NCBI/Uniprot data below describe general gene information for CXCL2. It may not necessarily be applicable to this product.
 NCBI GI #    127100
 NCBI GeneID    20310
 NCBI Accession #    P10889.2 [Other Products]
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 UniProt Primary Accession #    P10889 [Other Products]
 UniProt Related Accession #    P10889 [Other Products]
 Molecular Weight    10,621 Da
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 NCBI Official Full Name    C-X-C motif chemokine 2
 NCBI Official Synonym Full Names    chemokine (C-X-C motif) ligand 2
 NCBI Official Symbol    Cxcl2 [Similar Products]
 NCBI Official Synonym Symbols   
GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a
[Similar Products]
 NCBI Protein Information    C-X-C motif chemokine 2; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2
 UniProt Protein Name    C-X-C motif chemokine 2
 UniProt Synonym Protein Names   
Macrophage inflammatory protein 2; MIP2
 Protein Family    C-X-C motif chemokine
 UniProt Gene Name    Cxcl2 [Similar Products]
 UniProt Synonym Gene Names    Mip-2; Mip2; Scyb2; MIP2 [Similar Products]
 UniProt Entry Name    CXCL2_MOUSE
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 UniProt Comments for CXCL2    Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
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 Research Articles on CXCL2    1. Data indicate that levels of toll-like receptors TLR4/4-stimulated chemokines CXCL1 and CXCL2 were selectively enhanced in stressed macrophages via receptor-interacting protein kinase 1 (RIPK1).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with CXCL2 elisa kitDiseases associated with CXCL2 elisa kit
 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1000 publications with CXCL2 and Inflammation
 Necrosis Antibodies  >640 publications with CXCL2 and Necrosis
 Lung Diseases Antibodies  >383 publications with CXCL2 and Lung Diseases
 Disease Models, Animal Antibodies  >352 publications with CXCL2 and Disease Models, Animal
 Lung Injury Antibodies  >259 publications with CXCL2 and Lung Injury
 Pneumonia Antibodies  >172 publications with CXCL2 and Pneumonia
 Reperfusion Injury Antibodies  >115 publications with CXCL2 and Reperfusion Injury
 Wounds and Injuries Antibodies  >94 publications with CXCL2 and Wounds and Injuries
 Edema Antibodies  >92 publications with CXCL2 and Edema
 Skin Diseases Antibodies  >81 publications with CXCL2 and Skin Diseases
Organs/Tissues associated with CXCL2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >136 publications with CXCL2 and Bone Marrow
 Skin Antibodies  >97 publications with CXCL2 and Skin
 Spleen Antibodies  >72 publications with CXCL2 and Spleen
 Pancreas Antibodies  >42 publications with CXCL2 and Pancreas
 Mammary Gland Antibodies  >8 publications with CXCL2 and Mammary Gland
 Vagina Antibodies  >2 publications with CXCL2 and Vagina
 Uncharacterized Tissue Antibodies  >2 publications with CXCL2 and Uncharacterized Tissue
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