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MIP-2 elisa kit :: Mouse MIP-2 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS9424852
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
96 Tests  /  $450 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MIP-2, ELISA Kit

★Popular Item★
 Also Known As   

Mouse MIP-2 ELISA KIT

 Product Synonym Names    Mouse MIP-2
 Product Gene Name   

MIP-2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Species Reactivity    Mouse
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 Specificity    This assay recognizes both natural and recombinant mouse MIP-2. The factors listed below were prepared at 50nglml in Standard Isample Diluent and assayed for cross-reactivity and no significanl cross-reactivily or interference was observed.
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 Samples    Cell culture supernates, serum, and plasma
 Assay Type    Quantitative Sandwich
 Detection Range    15.6 pg/ml - 1000 pglml
 Sensitivity    7pg/mL.
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 Preparation and Storage    Store at 4 degree C
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MIP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MIP-2 purchase    MBS9424852 is a ready-to-use microwell, strip-or-full plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MIP-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 15.6 pg/ml - 1000 pglml in biological research samples containing MIP-2, with an estimated sensitivity of 7pg/mL. The ELISA analytical biochemical technique of the MBS9424852 kit is based on MIP-2 antibody-MIP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum, and plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MIP-2 elisa kit

   Principle of the Assay: This assay employs Ihe quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipelted into the wells and any MIP-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MIP-2 is added to the wells and binds to the combination of capture antibody-MIP-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A coloured product is formed in proportion to the amount of MIP-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MIP-2 standard dilutions and MIP-2 sample concentration determined.

Background/Introduction: Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2. was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line (1). A cDNA clone encoding the protein was isolated from this cell line and characterized (2). Based on its protein and DNA sequences. mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines (3). Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63% identity to that of mouse KC. another mouse alpha chemokine. Mouse MIP-2 is also 60% identical to human GRO and GRO (2). Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GRO, and chemokines. Since chemokines with protein sequence homology to human IL-S have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice (3. 4). A putative mouse homolog of the human IL-S receptor beta (IL-8 R ) has also been cloned. This receptor shows 71% identity to human IL-8 Rand 68% identity to human IL-8 R . Both mouse KC and MIP-2 bind mouse IL-8 R with high affinity (5). Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection (3, 4). Increased MIP-2 expression has been found to be associated with neutrophil influx in various inftammatory conditions (6 - 10).
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 Typical Testing Data/Standard Curve (for reference only) of MIP-2 elisa kit    MIP-2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS9424852. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MIP-2. It may not necessarily be applicable to this product.
 NCBI GI #    298582
 NCBI Accession #    AAB25756.1 [Other Products]
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 NCBI Official Full Name    macrophage inflammatory protein 2, MIP-2 {N-terminal}
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MIP-2 elisa kitPathways associated with MIP-2 elisa kit
 Reference Product  PubMed Publications
 FLNA elisa kit  >12 publications with MIP-2 and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with MIP-2 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with MIP-2 and Bone
 Connective Tissue Antibodies  >4 publications with MIP-2 and Connective Tissue
 Heart Antibodies  >3 publications with MIP-2 and Heart
 Muscle Antibodies  >3 publications with MIP-2 and Muscle
 Brain Antibodies  >3 publications with MIP-2 and Brain
 Vascular Antibodies  >2 publications with MIP-2 and Vascular
 Embryonic Tissue Antibodies  >2 publications with MIP-2 and Embryonic Tissue
 Ear Antibodies  >1 publications with MIP-2 and Ear
 Kidney Antibodies  >1 publications with MIP-2 and Kidney
 Umbilical Cord Antibodies  >1 publications with MIP-2 and Umbilical Cord
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