Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow MMP9 rightarrow Mouse MMP9  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • anti-Human IgE Antibody
 • DELGEF Antibody
 • 40S ribosomal protein S25 (RPS25) Antibody
 • KDM5C - Jarid1C - SMCX Antibody
 • H-2 class I histocompatibility antigen, D-K alpha chain (H2-... Recombinant Protein
 • Insulin Like Growth Factor1 (IGF1) ELISA Kit
 • AHCYL1 Antibody
 • 12-Lipoxygenase (LOX-12) ELISA Kit
 • PICK1 ELISA Kit
 • Carcinoembryonic Antigen (CEA) Antibody
 • Cholesteryl Ester Transfer Protein (CETP) ELISA Kit
 • GDF9 - GDF-9 Antibody
 • IgE ELISA Kit
 • CD239 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

MMP9 elisa kit :: Mouse MMP-9 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS175917
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $485 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MMP-9 (MMP9), ELISA Kit

 Also Known As   

Mouse MMP-9 PicoKine ELISA Kit

 Product Synonym Names    Matrix metalloproteinase-9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Collagenase Type 4 beta; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase B; Gelatinase beta; GelatinaseB; GELB; Macrophage gelatinase; MANDP2; Matrix metallopeptidase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collangenase); Matrix metalloproteinase 9; MMP 9; MMP-9; MMP9; MMP9_HUMAN; Type V collagenase
 Product Gene Name   

MMP9 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for P41245
 Species Reactivity    Mouse
Section Bar
 Specificity    Natural and recombinant mouse total MMP-9
Section Bar
 Samples    Cell culture supernates, serum and plasma (heparin)
 Assay Type    Sandwich
 Detection Range    156pg/ml-10,000pg/ml
 Sensitivity    <20pg/ml
Section Bar
 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of MMP9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP9 purchase    MBS175917 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the MMP-9 (MMP9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 156pg/ml-10,000pg/ml in biological research samples containing MMP9, with an estimated sensitivity of <20pg/ml. The ELISA analytical biochemical technique of the MBS175917 kit is based on MMP9 antibody-MMP9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP9. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Product Description specifically for MMP9 elisa kit

   Principle of the assay: mouse MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-9 has been precoated onto 96-well plates. Standards (CHO,A20-P730) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-9 amount of sample captured in plate.
Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allowangiogenesis and tumor invasion.2 Gelatinase B, a matrix metalloproteinase that has proteolytic activity againstconnective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture.3 MMP-9 is predominantly expressed in neutrophils,macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.4 The polymorphism of MMP-9 actsas a genetic factor for the development of smoking-induced pulmonary emphysema.5 The standard product used inthis kit is recombinant mouse MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of MMP9 elisa kit    MMP9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS175917
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for MMP9. It may not necessarily be applicable to this product.
 NCBI GI #    341940960
 NCBI GeneID    17395
 NCBI Accession #    P41245.2 [Other Products]
Section Bar
 UniProt Primary Accession #    P41245 [Other Products]
 UniProt Secondary Accession #    Q06788; Q80XI8; Q9DC02 [Other Products]
 UniProt Related Accession #    P41245 [Other Products]
 Molecular Weight    80,535 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Matrix metalloproteinase-9
 NCBI Official Synonym Full Names    matrix metallopeptidase 9
 NCBI Official Symbol    Mmp9 [Similar Products]
 NCBI Official Synonym Symbols   
Clg4b; MMP-9; B/MMP9; AW743869; pro-MMP-9
[Similar Products]
 NCBI Protein Information    matrix metalloproteinase-9; GELB; Gel B; gelatinase B; 92kD gelatinase; 92kDa gelatinase; 92 kDa gelatinase; collagenase type IVB; 92kD type IV collagenase; 92kDa type IV collagenase; 92 kDa type IV collagenase; matrix metalloproteinase 9
 UniProt Protein Name    Matrix metalloproteinase-9
 UniProt Synonym Protein Names   
92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
 Protein Family    Matrix metalloproteinase
 UniProt Gene Name    Mmp9 [Similar Products]
 UniProt Synonym Gene Names    Clg4b; MMP-9; GELB [Similar Products]
 UniProt Entry Name    MMP9_MOUSE
Section Bar
 UniProt Comments for MMP9    MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.35; Secreted, signal peptide

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; protein complex; extracellular region

Molecular Function: peptidase activity; identical protein binding; zinc ion binding; hydrolase activity; metallopeptidase activity; fibronectin binding; metal ion binding; metalloendopeptidase activity; endopeptidase activity; protein complex binding

Biological Process: positive regulation of keratinocyte migration; response to drug; ossification; extracellular matrix organization and biogenesis; positive regulation of apoptosis; positive regulation of leukocyte migration; tissue remodeling; positive regulation of synaptic plasticity; proteolysis; protein oligomerization; collagen catabolic process; positive regulation of angiogenesis; negative regulation of fibroblast proliferation; response to oxidative stress; leukocyte migration; skeletal development; embryo implantation; transformation of host cell by virus
Table BarTOPTable Bar
 Research Articles on MMP9    1. TNF-alpha-induced MMP-9 expression is mediated through a c-Src-dependent PDGFR transactivation.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with MMP9 elisa kitDiseases associated with MMP9 elisa kit
 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >497 publications with MMP9 and Inflammation
 Neoplasm Invasiveness Antibodies  >403 publications with MMP9 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >355 publications with MMP9 and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >166 publications with MMP9 and Breast Neoplasms
 Liver Diseases Antibodies  >148 publications with MMP9 and Liver Diseases
 Aortic Diseases Antibodies  >98 publications with MMP9 and Aortic Diseases
 Carcinoma, Squamous Cell Antibodies  >96 publications with MMP9 and Carcinoma, Squamous Cell
 Aortic Aneurysm Antibodies  >93 publications with MMP9 and Aortic Aneurysm
 Aortic Aneurysm, Abdominal Antibodies  >76 publications with MMP9 and Aortic Aneurysm, Abdominal
 Carcinoma, Hepatocellular Antibodies  >72 publications with MMP9 and Carcinoma, Hepatocellular
Organs/Tissues associated with MMP9 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >810 publications with MMP9 and Blood
 Lung Antibodies  >356 publications with MMP9 and Lung
 Bone Antibodies  >319 publications with MMP9 and Bone
 Brain Antibodies  >265 publications with MMP9 and Brain
 Liver Antibodies  >211 publications with MMP9 and Liver
 Heart Antibodies  >204 publications with MMP9 and Heart
 Bone Marrow Antibodies  >135 publications with MMP9 and Bone Marrow
 Skin Antibodies  >118 publications with MMP9 and Skin
 Embryonic Tissue Antibodies  >111 publications with MMP9 and Embryonic Tissue
 Eye Antibodies  >85 publications with MMP9 and Eye
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions