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MMP2 elisa kit :: Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS454416
 Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $505 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,275 +2 FREE 8GB USB
10x96-Strip-Wells  /  $4,420 +5 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Matrix Metalloproteinase 2 (MMP2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit

 Product Synonym Names    CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
 Product Gene Name   

MMP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 3D Structure    ModBase 3D Structure for P33434
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MMP2. No significant cross-reactivity or interference between MMP2 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates or other biological fluids
 Assay Type    Quantitative Sandwich
 Detection Range    0.156-10ng/mL
 Sensitivity    <0.065ng/mL.
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
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 Preparation and Storage    For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MMP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MMP2 purchase    MBS454416 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix Metalloproteinase 2 (MMP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing MMP2, with an estimated sensitivity of <0.065ng/mL. The ELISA analytical biochemical technique of the MBS454416 kit is based on MMP2 antibody-MMP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates or other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MMP2 elisa kit

   Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MMP2 in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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  Typical Testing Data/Standard Curve (for reference only) of MMP2 elisa kit    MMP2 elisa kit  Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS454416. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MMP2. It may not necessarily be applicable to this product.
 NCBI GI #    6678902
 NCBI GeneID    17390
 NCBI Accession #    NP_032636.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_008610.3 [Other Products]
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 UniProt Primary Accession #    P33434 [Other Products]
 UniProt Related Accession #    P33434 [Other Products]
 Molecular Weight    65,883 Da
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 NCBI Official Full Name    72 kDa type IV collagenase preproprotein
 NCBI Official Synonym Full Names    matrix metallopeptidase 2
 NCBI Official Symbol    Mmp2 [Similar Products]
 NCBI Official Synonym Symbols   
GelA; Clg4a; MMP-2
[Similar Products]
 NCBI Protein Information    72 kDa type IV collagenase
 UniProt Protein Name    72 kDa type IV collagenase
 UniProt Synonym Protein Names   
72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2
 Protein Family    Matrix metalloproteinase
 UniProt Gene Name    Mmp2 [Similar Products]
 UniProt Synonym Gene Names    MMP-2 [Similar Products]
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 NCBI Summary for MMP2    This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin and elastin. Mice lacking the encoded protein exhibit suppressed angiogenesis and attenuated features of human multicentric osteolysis with arthritis including abnormal skeletal and craniofacial development. [provided by RefSeq, Feb 2016]
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 UniProt Comments for MMP2    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues ().
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 Research Articles on MMP2    1. MMP-2 potentiates shear-induced platelet activation by enhancing thrombus formation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MMP2 elisa kitPathways associated with MMP2 elisa kit
 Reference Product  PubMed Publications
 Jun elisa kit  >461 publications with MMP2 and Jun
 Timp2 elisa kit  >382 publications with MMP2 and Timp2
 Timp1 elisa kit  >359 publications with MMP2 and Timp1
 Vegfa elisa kit  >144 publications with MMP2 and Vegfa
 Timp3 elisa kit  >90 publications with MMP2 and Timp3
 Src elisa kit  >65 publications with MMP2 and Src
 Trp53 elisa kit  >24 publications with MMP2 and Trp53
 Col18a1 elisa kit  >13 publications with MMP2 and Col18a1
 Dcn elisa kit  >8 publications with MMP2 and Dcn
 Igfbp3 elisa kit  >7 publications with MMP2 and Igfbp3
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
Diseases associated with MMP2 elisa kitOrgans/Tissues associated with MMP2 elisa kit
 Disease Name  Pubmed Publications
 Neoplasm Invasiveness Antibodies  >790 publications with MMP2 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >631 publications with MMP2 and Neoplasm Metastasis
 Inflammation Antibodies  >461 publications with MMP2 and Inflammation
 Fibrosis Antibodies  >343 publications with MMP2 and Fibrosis
 Disease Progression Antibodies  >299 publications with MMP2 and Disease Progression
 Necrosis Antibodies  >259 publications with MMP2 and Necrosis
 Breast Neoplasms Antibodies  >249 publications with MMP2 and Breast Neoplasms
 Bone Diseases Antibodies  >164 publications with MMP2 and Bone Diseases
 Carcinoma, Hepatocellular Antibodies  >155 publications with MMP2 and Carcinoma, Hepatocellular
 Aortic Diseases Antibodies  >135 publications with MMP2 and Aortic Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >535 publications with MMP2 and Muscle
 Lung Antibodies  >454 publications with MMP2 and Lung
 Bone Antibodies  >341 publications with MMP2 and Bone
 Heart Antibodies  >324 publications with MMP2 and Heart
 Brain Antibodies  >310 publications with MMP2 and Brain
 Connective Tissue Antibodies  >196 publications with MMP2 and Connective Tissue
 Skin Antibodies  >191 publications with MMP2 and Skin
 Kidney Antibodies  >187 publications with MMP2 and Kidney
 Embryonic Tissue Antibodies  >165 publications with MMP2 and Embryonic Tissue
 Eye Antibodies  >135 publications with MMP2 and Eye
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