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MEPE elisa kit :: Mouse Matrix extracellular phosphoglycoprotein ELISA Kit

Scan QR to view Datasheet Catalog #    MBS721044
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Matrix extracellular phosphoglycoprotein (MEPE), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Matrix extracellular phosphoglycoprotein ELISA Kit

 Product Gene Name   

MEPE elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    605912
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    2.5-50ng/mL
 Sensitivity    0.1ng/mL
 Intended Uses    This MEPE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MEPE. This ELISA kit for research use only, not for therapeutic or diagnostic applications
 !Intended Uses    This MEPE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MEPE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MEPE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MEPE purchase    MBS721044 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix extracellular phosphoglycoprotein (MEPE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 2.5-50ng/mL in biological research samples containing MEPE, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS721044 kit is based on MEPE antibody-MEPE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MEPE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MEPE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MEPE elisa kit

   Principle of the Assay: MEPE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MEPE antibody and an MEPE-HRP conjugate. The assay sample and buffer are incubated together with MEPE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MEPE concentration since MEPE from samples and MEPE-HRP conjugate compete for the anti-MEPE antibody binding site. Since the number of sites is limited, as more sites are occupied by MEPE from the sample, fewer sites are left to bind MEPE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MEPE concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MEPE elisa kit    Cell Biology
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 Typical Testing Data/Standard Curve (for reference only) of MEPE elisa kit    MEPE elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS721044. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MEPE. It may not necessarily be applicable to this product.
 NCBI GI #    118764291
 NCBI GeneID    56955
 NCBI Accession #    AAI28159.1 [Other Products]
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 UniProt Secondary Accession #    A1A4X9; A8MTA3; D2CFR4; F5H5C5 [Other Products]
 UniProt Related Accession #    Q9NQ76 [Other Products]
 Molecular Weight    45,291 Da
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 NCBI Official Full Name    Matrix extracellular phosphoglycoprotein
 NCBI Official Synonym Full Names    matrix extracellular phosphoglycoprotein
 NCBI Official Symbol    MEPE [Similar Products]
 NCBI Official Synonym Symbols   
OF45
[Similar Products]
 NCBI Protein Information    matrix extracellular phosphoglycoprotein; osteoblast/osteocyte factor 45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone)
 UniProt Protein Name    Matrix extracellular phosphoglycoprotein
 UniProt Synonym Protein Names   
Osteoblast/osteocyte factor 45; OF45
 Protein Family    Matrix extracellular phosphoglycoprotein
 UniProt Gene Name    MEPE [Similar Products]
 UniProt Synonym Gene Names    OF45 [Similar Products]
 UniProt Entry Name    MEPE_HUMAN
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 NCBI Summary for MEPE    This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
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 UniProt Comments for MEPE    MEPE: Promotes renal phosphate excretion and modulates mineralization. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: proteinaceous extracellular matrix

Molecular Function: protein binding; extracellular matrix structural constituent

Biological Process: negative regulation of bone mineralization; biomineral formation; regulation of bone remodeling; skeletal development
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 Research Articles on MEPE    1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MEPE elisa kitDiseases associated with MEPE elisa kit
 Reference Product  PubMed Publications
 DMP1 elisa kit  >56 publications with MEPE and DMP1
 PHEX elisa kit  >46 publications with MEPE and PHEX
 FGF23 elisa kit  >27 publications with MEPE and FGF23
 IBSP elisa kit  >26 publications with MEPE and IBSP
 DSPP elisa kit  >26 publications with MEPE and DSPP
 SPP1 elisa kit  >15 publications with MEPE and SPP1
 SFRP4 elisa kit  >10 publications with MEPE and SFRP4
 GALNT3 elisa kit  >4 publications with MEPE and GALNT3
 Disease Name  Pubmed Publications
 Osteomalacia Antibodies  >34 publications with MEPE and Osteomalacia
 Kidney Diseases Antibodies  >31 publications with MEPE and Kidney Diseases
 Bone Diseases, Metabolic Antibodies  >28 publications with MEPE and Bone Diseases, Metabolic
 Familial Hypophosphatemic Rickets Antibodies  >16 publications with MEPE and Familial Hypophosphatemic Rickets
 Fractures, Bone Antibodies  >7 publications with MEPE and Fractures, Bone
 Renal Insufficiency Antibodies  >6 publications with MEPE and Renal Insufficiency
 Death Antibodies  >4 publications with MEPE and Death
 Calcinosis Antibodies  >4 publications with MEPE and Calcinosis
 Brain Diseases Antibodies  >2 publications with MEPE and Brain Diseases
 Hypercalciuria Antibodies  >1 publications with MEPE and Hypercalciuria
Organs/Tissues associated with MEPE elisa kit
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >131 publications with MEPE and Bone
 Brain Antibodies  >3 publications with MEPE and Brain
 Placenta Antibodies  >2 publications with MEPE and Placenta
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