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Mcpt4 elisa kit :: Mouse Mcpt4 (Mast cell protease 4) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7606603
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $300 +1 FREE 8GB USB
96-Strip-Wells  /  $415 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mcpt4 (Mast cell protease 4), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Mcpt4 (Mast cell protease 4) ELISA Kit

 Product Synonym Names    Mcpt4; mast cell protease 4; MSMCP; Serosal mast cell protease
 Product Gene Name   

Mcpt4 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS7606603 COA    COA PDF
 3D Structure    ModBase 3D Structure for P21812
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mcpt4 . No significant cross-reactivity or interference between Mcpt4 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    46.875-3000pg/ml
 Sensitivity    <28.125pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mcpt4 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
 Inter-assay Precision    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mcpt4 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
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 Preparation and Storage    Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Mcpt4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Mcpt4 purchase    MBS7606603 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mcpt4 (Mast cell protease 4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 46.875-3000pg/ml in biological research samples containing Mcpt4, with an estimated sensitivity of <28.125pg/ml. The ELISA analytical biochemical technique of the MBS7606603 kit is based on Mcpt4 antibody-Mcpt4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Mcpt4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Mcpt4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Mcpt4 elisa kit

   Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Mcpt4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Mcpt4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mcpt4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mcpt4 can be calculated.
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 Typical Testing Data/Standard Curve (for reference only) of Mcpt4 elisa kit    Mcpt4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7606603. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Mcpt4. It may not necessarily be applicable to this product.
 NCBI GI #    114205406
 NCBI GeneID    17227
 NCBI Accession #    NP_034909.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_010779.2 [Other Products]
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 UniProt Primary Accession #    P21812 [Other Products]
 UniProt Secondary Accession #    Q9EPQ9; Q9EQT2 [Other Products]
 UniProt Related Accession #    P21812 [Other Products]
 Molecular Weight    27,204 Da
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 NCBI Official Full Name    mast cell protease 4
 NCBI Official Synonym Full Names    mast cell protease 4
 NCBI Official Symbol    Mcpt4 [Similar Products]
 NCBI Official Synonym Symbols   
Mcp4; Mcp-4; MMCP-4; MMCP-4A; MMCP-4B
[Similar Products]
 NCBI Protein Information    mast cell protease 4
 UniProt Protein Name    Mast cell protease 4
 UniProt Synonym Protein Names   
MSMCP; Myonase; Serosal mast cell protease
 Protein Family    Mast cell protease
 UniProt Gene Name    Mcpt4 [Similar Products]
 UniProt Synonym Gene Names    mMCP-4 [Similar Products]
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 UniProt Comments for Mcpt4    Mcpt4: Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta- protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 14 C3|14 28.19 cM

Cellular Component: intracellular

Molecular Function: peptidase activity; serine-type endopeptidase activity

Biological Process: protein processing; proteolysis; regulation of angiotensin levels in blood
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 Research Articles on Mcpt4    1. study supports a role for mMCP-4 in the early inflammatory phases of the disease in a mouse model of MS.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with Mcpt4 elisa kitOrgans/Tissues associated with Mcpt4 elisa kit
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with Mcpt4 and Necrosis
 Lung Diseases Antibodies  >1 publications with Mcpt4 and Lung Diseases
 Cardiovascular Diseases Antibodies  >1 publications with Mcpt4 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >1 publications with Mcpt4 and Bone Marrow
 Other Antibodies  >1 publications with Mcpt4 and Other
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