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MEP1a elisa kit :: Mouse Meprin A Alpha (MEP1a) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2020854 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $260 +1 FREE 8GB USB
48-Strip-Wells  /  $465 +1 FREE 8GB USB
96-Strip-Wells  /  $625 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,530 +3 FREE 8GB USB
10x96-Strip-Wells  /  $4,565 +5 FREE 8GB USB
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 Product Name   

Meprin A Alpha (MEP1a), ELISA Kit

★Popular Item★
 Also Known As   

Meprin A Alpha (MEP1a) ELISA Kit

 Product Synonym Names    PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase
 Product Gene Name   

MEP1a elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    600388
 3D Structure    ModBase 3D Structure for Q16819
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a).
No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates and other biological fluids
 Assay Type    Sandwich
 Detection Range    62.5-4000pg/mL
 Sensitivity    Typically less than 26.4pg/mL.
 Precision    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
 Assay Procedure Summary    1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;
8. Add 50uL Stop Solution. Read at 450nm immediately.
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 Preparation and Storage    For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MEP1a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MEP1a purchase    MBS2020854 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Meprin A Alpha (MEP1a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 62.5-4000pg/mL in biological research samples containing MEP1a, with an estimated sensitivity of Typically less than 26.4pg/mL. The ELISA analytical biochemical technique of the MBS2020854 kit is based on MEP1a antibody-MEP1a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MEP1a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MEP1a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates and other biological fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MEP1a elisa kit

   Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Alpha (MEP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Alpha (MEP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Alpha (MEP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meprin A Alpha (MEP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of MEP1a elisa kit    MEP1a elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2020854. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MEP1a. It may not necessarily be applicable to this product.
 NCBI GI #    153070262
 NCBI GeneID    4224
 NCBI Accession #    NP_005579.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005588.2 [Other Products]
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 UniProt Primary Accession #    Q16819 [Other Products]
 UniProt Secondary Accession #    Q8TDC9; Q9H1R1; A2RRM4; B0AZP9; B2RCS2 [Other Products]
 UniProt Related Accession #    Q16819 [Other Products]
 Molecular Weight    84,419 Da
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 NCBI Official Full Name    meprin A subunit alpha
 NCBI Official Synonym Full Names    meprin A, alpha (PABA peptide hydrolase)
 NCBI Official Symbol    MEP1A [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    meprin A subunit alpha
 UniProt Protein Name    Meprin A subunit alpha
 UniProt Synonym Protein Names   
Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha
 Protein Family    Meprin
 UniProt Gene Name    MEP1A [Similar Products]
 UniProt Entry Name    MEP1A_HUMAN
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 UniProt Comments for MEP1a    MEP1A: Belongs to the peptidase M12A family.

Protein type: Membrane protein, integral; Protease; EC

Chromosomal Location of Human Ortholog: 6p12-p11

Cellular Component: extracellular space; integral to plasma membrane; meprin A complex

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity

Biological Process: digestion; proteolysis
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 Research Articles on MEP1a    1. Variants rs17468190 (G/T) in the MEP1A gene are associated with glucose and insulin metabolism in overweight/obese central European women with polycystic ovary syndrome.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MEP1a elisa kitPathways associated with MEP1a elisa kit
 Reference Product  PubMed Publications
 MEP1B elisa kit  >5 publications with MEP1a and MEP1B
 CDX2 elisa kit  >2 publications with MEP1a and CDX2
 PTH elisa kit  >1 publications with MEP1a and PTH
 Products by Pathway  Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
Diseases associated with MEP1a elisa kitOrgans/Tissues associated with MEP1a elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >6 publications with MEP1a and Inflammation
 Obesity Antibodies  >1 publications with MEP1a and Obesity
 Liver Diseases Antibodies  >1 publications with MEP1a and Liver Diseases
 Fibrosis Antibodies  >1 publications with MEP1a and Fibrosis
 Kidney Diseases Antibodies  >1 publications with MEP1a and Kidney Diseases
 Necrosis Antibodies  >1 publications with MEP1a and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >6 publications with MEP1a and Intestine
 Liver Antibodies  >1 publications with MEP1a and Liver
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