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UCP1 elisa kit :: Mouse Mitochondrial brown fat uncoupling protein 1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2606225
Typical Standard Curve/Testing Data
Unit / Price
48-Strip-Wells  /  $275 +1 FREE 8GB USB
96-Strip-Wells  /  $435 +1 FREE 8GB USB
5x96-Strip-Wells  /  $1,755 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,150 +3 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mitochondrial brown fat uncoupling protein 1 (UCP1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit

 Product Gene Name   

UCP1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    113730
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 Species Reactivity    Mouse
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 Specificity    No cross-reaction with other factors.
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 Samples    Serum, plasma or cell culture supernatant
 Assay Type    Quantitative Sandwich
 Detection Range    10 ng/ml-0.156ng/ml
 Sensitivity    Up to 0.05 ng/ml.
 Intra-assay Precision    <= 8%
 Inter-assay Precision    <= 12%
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of UCP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for UCP1 purchase    MBS2606225 is a ready-to-use microwell, strip plate Quantitative Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 10 ng/ml-0.156ng/ml in biological research samples containing UCP1, with an estimated sensitivity of Up to 0.05 ng/ml. The ELISA analytical biochemical technique of the MBS2606225 kit is based on UCP1 antibody-UCP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UCP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UCP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma or cell culture supernatant. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for UCP1 elisa kit

   Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse UCP1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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 Typical Standard Curve/Testing Data of UCP1 elisa kit    UCP1 elisa kit Typical Standard Curve/Testing Data image
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Sample Manual Insert of MBS2606225. Click to request current manual
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NCBI/Uniprot data below describe general gene information for UCP1. It may not necessarily be applicable to this product.
 NCBI GI #    11225256
 NCBI GeneID    7350
 NCBI Accession #    NP_068605.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021833.4 [Other Products]
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 UniProt Secondary Accession #    Q13218; Q4KMZ3; Q68G66 [Other Products]
 UniProt Related Accession #    P25874 [Other Products]
 Molecular Weight    33,005 Da
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 NCBI Official Full Name    mitochondrial brown fat uncoupling protein 1
 NCBI Official Synonym Full Names    uncoupling protein 1 (mitochondrial, proton carrier)
 NCBI Official Symbol    UCP1 [Similar Products]
 NCBI Official Synonym Symbols   
UCP; SLC25A7
[Similar Products]
 NCBI Protein Information    mitochondrial brown fat uncoupling protein 1
 UniProt Protein Name    Mitochondrial brown fat uncoupling protein 1
 UniProt Synonym Protein Names   
Solute carrier family 25 member 7; Thermogenin
 Protein Family    Mitochondrial brown fat uncoupling protein
 UniProt Gene Name    UCP1 [Similar Products]
 UniProt Synonym Gene Names    SLC25A7; UCP; UCP 1 [Similar Products]
 UniProt Entry Name    UCP1_HUMAN
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 NCBI Summary for UCP1    Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq, Jul 2008]
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 UniProt Comments for UCP1    UCP1: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q28-q31

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: oxidative phosphorylation uncoupler activity; structural constituent of ribosome

Biological Process: brown fat cell differentiation; cellular metabolic process; mitochondrial transport; proton transport; regulation of transcription from RNA polymerase II promoter; translation

Disease: Obesity
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 Research Articles on UCP1    1. this study reveals that human adipose-derived stromal/progenitor cells can be readily differentiated into beige adipocytes that, upon activation, undergo uncoupling protein 1-dependent thermogenesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with UCP1 elisa kitPathways associated with UCP1 elisa kit
 Reference Product  PubMed Publications
 PPARGC1A elisa kit  >68 publications with UCP1 and PPARGC1A
 CIDEA elisa kit  >34 publications with UCP1 and CIDEA
 NPY elisa kit  >19 publications with UCP1 and NPY
 ADRB3 elisa kit  >13 publications with UCP1 and ADRB3
 FNDC5 elisa kit  >9 publications with UCP1 and FNDC5
 INS elisa kit  >8 publications with UCP1 and INS
 LEP elisa kit  >8 publications with UCP1 and LEP
 PPARG elisa kit  >7 publications with UCP1 and PPARG
 PPARA elisa kit  >5 publications with UCP1 and PPARA
 RETN elisa kit  >3 publications with UCP1 and RETN
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Mitochondrial Uncoupling Proteins Pathway antibodies  Mitochondrial Uncoupling Proteins Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 PPAR Signaling Pathway antibodies  PPAR Signaling Pathway Diagram
 Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway antibodies  Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway Diagram
 The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies  The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram
Diseases associated with UCP1 elisa kitOrgans/Tissues associated with UCP1 elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >617 publications with UCP1 and Obesity
 Insulin Resistance Antibodies  >133 publications with UCP1 and Insulin Resistance
 Weight Gain Antibodies  >123 publications with UCP1 and Weight Gain
 Weight Loss Antibodies  >89 publications with UCP1 and Weight Loss
 Fatty Liver Antibodies  >75 publications with UCP1 and Fatty Liver
 Inflammation Antibodies  >56 publications with UCP1 and Inflammation
 Cardiovascular Diseases Antibodies  >38 publications with UCP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >34 publications with UCP1 and Disease Models, Animal
 Vascular Diseases Antibodies  >30 publications with UCP1 and Vascular Diseases
 Liver Diseases Antibodies  >30 publications with UCP1 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >1125 publications with UCP1 and Adipose Tissue
 Connective Tissue Antibodies  >773 publications with UCP1 and Connective Tissue
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