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UCP2 elisa kit :: Mouse Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7234483
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Mitochondrial uncoupling protein 2 (UCP2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit

 Product Gene Name   

UCP2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    gene 607447
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of UCP2. No significant cross-reactivity or interference between UCP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UCP2 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    0.5-10ng/mL
 Sensitivity    0.1ng/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of UCP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for UCP2 purchase    MBS7234483 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mitochondrial uncoupling protein 2 (UCP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.5-10ng/mL in biological research samples containing UCP2, with an estimated sensitivity of 0.1ng/mL. The ELISA analytical biochemical technique of the MBS7234483 kit is based on UCP2 antibody-UCP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UCP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UCP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for UCP2 elisa kit

   Intended Uses: This UCP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse UCP2.

Principle of the Assay: UCP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UCP2 antibody and an UCP2-HRP conjugate. The assay sample and buffer are incubated together with UCP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UCP2 concentration since UCP2 from samples and UCP2-HRP conjugate compete for the anti-UCP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by UCP2 from the sample, fewer sites are left to bind UCP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UCP2 concentration in each sample is interpolated from this standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of UCP2 elisa kit    UCP2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7234483. Click to request current manual
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NCBI/Uniprot data below describe general gene information for UCP2. It may not necessarily be applicable to this product.
 NCBI GI #    13259541
 NCBI GeneID    7351
 NCBI Accession #    NP_003346.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003355.2 [Other Products]
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 UniProt Secondary Accession #    Q4PJH8; Q53HM3 [Other Products]
 UniProt Related Accession #    P55851 [Other Products]
 Molecular Weight    33,229 Da
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 NCBI Official Full Name    mitochondrial uncoupling protein 2
 NCBI Official Synonym Full Names    uncoupling protein 2 (mitochondrial, proton carrier)
 NCBI Official Symbol    UCP2 [Similar Products]
 NCBI Official Synonym Symbols   
UCPH; BMIQ4; SLC25A8
[Similar Products]
 NCBI Protein Information    mitochondrial uncoupling protein 2; UCP 2; solute carrier family 25 member 8
 UniProt Protein Name    Mitochondrial uncoupling protein 2
 UniProt Synonym Protein Names   
Solute carrier family 25 member 8; UCPH
 UniProt Gene Name    UCP2 [Similar Products]
 UniProt Synonym Gene Names    SLC25A8; UCP 2 [Similar Products]
 UniProt Entry Name    UCP2_HUMAN
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 NCBI Summary for UCP2    Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]
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 UniProt Comments for UCP2    UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.

Protein type: Membrane protein, multi-pass; Mitochondrial; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: mitochondrial inner membrane; integral to membrane

Biological Process: cellular metabolic process; proton transport; response to superoxide; regulation of mitochondrial membrane potential; cellular response to insulin stimulus; cellular response to amino acid starvation; transport; response to hypoxia; female pregnancy; negative regulation of apoptosis; mitochondrial transport; aging

Disease: Body Mass Index Quantitative Trait Locus 4
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 Research Articles on UCP2    1. In various tumor types protein levels of UCP2 are significantly higher in tumor tissues than that in the adjacent normal tissues.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with UCP2 elisa kitPathways associated with UCP2 elisa kit
 Reference Product  PubMed Publications
 UCP1 elisa kit  >309 publications with UCP2 and UCP1
 PPARG elisa kit  >69 publications with UCP2 and PPARG
 INS elisa kit  >48 publications with UCP2 and INS
 SIRT1 elisa kit  >14 publications with UCP2 and SIRT1
 LEP elisa kit  >9 publications with UCP2 and LEP
 NOS3 elisa kit  >7 publications with UCP2 and NOS3
 NRF1 elisa kit  >7 publications with UCP2 and NRF1
 GHRL elisa kit  >2 publications with UCP2 and GHRL
 FOXA2 elisa kit  >1 publications with UCP2 and FOXA2
 Products by Pathway  Pathway Diagram
 Electron Transport Chain Pathway antibodies  Electron Transport Chain Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Mitochondrial Uncoupling Proteins Pathway antibodies  Mitochondrial Uncoupling Proteins Pathway Diagram
 Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway antibodies  Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway Diagram
 The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies  The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram
 The Fatty Acid Cycling Model Pathway antibodies  The Fatty Acid Cycling Model Pathway Diagram
 The Proton Buffering Model Pathway antibodies  The Proton Buffering Model Pathway Diagram
Diseases associated with UCP2 elisa kitOrgans/Tissues associated with UCP2 elisa kit
 Disease Name  Pubmed Publications
 Obesity Antibodies  >341 publications with UCP2 and Obesity
 Fatty Liver Antibodies  >100 publications with UCP2 and Fatty Liver
 Insulin Resistance Antibodies  >97 publications with UCP2 and Insulin Resistance
 Diabetes Mellitus, Type 2 Antibodies  >95 publications with UCP2 and Diabetes Mellitus, Type 2
 Cardiovascular Diseases Antibodies  >91 publications with UCP2 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >80 publications with UCP2 and Disease Models, Animal
 Inflammation Antibodies  >65 publications with UCP2 and Inflammation
 Nervous System Diseases Antibodies  >61 publications with UCP2 and Nervous System Diseases
 Weight Gain Antibodies  >55 publications with UCP2 and Weight Gain
 Necrosis Antibodies  >53 publications with UCP2 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Adipose Tissue Antibodies  >350 publications with UCP2 and Adipose Tissue
 Muscle Antibodies  >322 publications with UCP2 and Muscle
 Blood Antibodies  >255 publications with UCP2 and Blood
 Connective Tissue Antibodies  >238 publications with UCP2 and Connective Tissue
 Liver Antibodies  >199 publications with UCP2 and Liver
 Brain Antibodies  >142 publications with UCP2 and Brain
 Pancreas Antibodies  >113 publications with UCP2 and Pancreas
 Heart Antibodies  >100 publications with UCP2 and Heart
 Vascular Antibodies  >48 publications with UCP2 and Vascular
 Kidney Antibodies  >43 publications with UCP2 and Kidney
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