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MYO/MB elisa kit :: Mouse Myoglobin ELISA Kit

Scan QR to view Datasheet Catalog #    MBS724191
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myoglobin (MYO/MB), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Myoglobin ELISA Kit

 Product Gene Name   

MYO/MB elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    160000
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MYO/MB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MYO/MB purchase    MBS724191 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myoglobin (MYO/MB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing MYO/MB, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS724191 kit is based on MYO/MB antibody-MYO/MB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYO/MB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYO/MB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for MYO/MB elisa kit

   For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Intended Uses: This MYO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: MYO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYO antibody and an MYO-HRP conjugate. The assay sample and buffer are incubated together with MYO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO concentration since MYO from samples and MYO-HRP conjugate compete for the anti-MYO antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO from the sample, fewer sites are left to bind MYO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MYO/MB elisa kit    Cardiovascular
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 Typical Testing Data/Standard Curve (for reference only) of MYO/MB elisa kit    MYO/MB elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS724191. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MYO/MB. It may not necessarily be applicable to this product.
 NCBI GI #    44955888
 NCBI GeneID    4151
 NCBI Accession #    NP_976312.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_203378.1 [Other Products]
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 UniProt Secondary Accession #    Q52H51; Q5THY7 [Other Products]
 UniProt Related Accession #    P02144 [Other Products]
 Molecular Weight    17,184 Da
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 NCBI Official Full Name    myoglobin
 NCBI Official Synonym Full Names    myoglobin
 NCBI Official Symbol    MB [Similar Products]
 NCBI Official Synonym Symbols   
PVALB; myoglobgin
[Similar Products]
 NCBI Protein Information    myoglobin
 UniProt Protein Name    Myoglobin
 Protein Family    Myoglobin
 UniProt Gene Name    MB [Similar Products]
 UniProt Entry Name    MYG_HUMAN
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 NCBI Summary for MYO/MB    This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MYO/MB    MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hypoxia; brown fat cell differentiation; response to hormone stimulus; enucleate erythrocyte differentiation
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 Research Articles on MYO/MB    1. the novel cancer-associated MB splice variants exhibited increased expression in tumor cells subjected to experimental hypoxia; the novel gene regulatory mechanisms unveiled in this study support the idea of a non-canonical role of MB during carcinogenesis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MYO/MB elisa kitPathways associated with MYO/MB elisa kit
 Reference Product  PubMed Publications
 ALB elisa kit  >14 publications with MYO/MB and ALB
 HBB elisa kit  >12 publications with MYO/MB and HBB
 CKM elisa kit  >11 publications with MYO/MB and CKM
 HPR elisa kit  >10 publications with MYO/MB and HPR
 TNNT2 elisa kit  >4 publications with MYO/MB and TNNT2
 TNNI3 elisa kit  >2 publications with MYO/MB and TNNI3
 VCAM1 elisa kit  >2 publications with MYO/MB and VCAM1
 PGAM2 elisa kit  >1 publications with MYO/MB and PGAM2
 Products by Pathway  Pathway Diagram
 Effects Of Nitric Oxide Pathway antibodies  Effects Of Nitric Oxide Pathway Diagram
Diseases associated with MYO/MB elisa kitOrgans/Tissues associated with MYO/MB elisa kit
 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >5873 publications with MYO/MB and Heart Diseases
 Myocardial Infarction Antibodies  >3895 publications with MYO/MB and Myocardial Infarction
 Congenital Abnormalities Antibodies  >2159 publications with MYO/MB and Congenital Abnormalities
 Brain Diseases Antibodies  >1770 publications with MYO/MB and Brain Diseases
 Necrosis Antibodies  >1363 publications with MYO/MB and Necrosis
 Adenocarcinoma Antibodies  >972 publications with MYO/MB and Adenocarcinoma
 Lung Diseases Antibodies  >939 publications with MYO/MB and Lung Diseases
 Inflammation Antibodies  >866 publications with MYO/MB and Inflammation
 Lung Neoplasms Antibodies  >818 publications with MYO/MB and Lung Neoplasms
 Kidney Diseases Antibodies  >549 publications with MYO/MB and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >5165 publications with MYO/MB and Heart
 Muscle Antibodies  >3549 publications with MYO/MB and Muscle
 Vascular Antibodies  >2221 publications with MYO/MB and Vascular
 Lung Antibodies  >1606 publications with MYO/MB and Lung
 Liver Antibodies  >1138 publications with MYO/MB and Liver
 Nerve Antibodies  >821 publications with MYO/MB and Nerve
 Prostate Antibodies  >613 publications with MYO/MB and Prostate
 Eye Antibodies  >536 publications with MYO/MB and Eye
 Mouth Antibodies  >347 publications with MYO/MB and Mouth
 Embryonic Tissue Antibodies  >313 publications with MYO/MB and Embryonic Tissue
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