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MYH1 elisa kit :: Mouse Myosin-1 (MYH1) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS7215467
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $455 +1 FREE 8GB USB
96-Strip-Wells  /  $660 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,925 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,490 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin-1 (MYH1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Myosin-1 (MYH1) ELISA Kit

 Product Gene Name   

MYH1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    160730
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 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of MYH1. No significant cross-reactivity or interference between MYH1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYH1 and all the analogues, therefore, cross reaction may still exist in some cases.
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    100-2500pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This MYH1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYH1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of MYH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MYH1 purchase    MBS7215467 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myosin-1 (MYH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 100-2500pg/mL in biological research samples containing MYH1, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS7215467 kit is based on MYH1 antibody-MYH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MYH1 elisa kit

   Principle of the assay: MYH1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYH1 antibody and an MYH1-HRP conjugate. The assay sample and buffer are incubated together with MYH1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYH1 concentration since MYH1 from samples and MYH1-HRP conjugate compete for the anti-MYH1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYH1 from the sample, fewer sites are left to bind MYH1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYH1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for MYH1 elisa kit    Signal Transduction
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 Typical Testing Data/Standard Curve (for reference only) of MYH1 elisa kit    MYH1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS7215467. Click to request current manual
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NCBI/Uniprot data below describe general gene information for MYH1. It may not necessarily be applicable to this product.
 NCBI GI #    115527082
 NCBI GeneID    4619
 NCBI Accession #    NP_005954.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_005963.3 [Other Products]
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 UniProt Secondary Accession #    Q14CA4; Q9Y622 [Other Products]
 UniProt Related Accession #    P12882 [Other Products]
 Molecular Weight    223,145 Da
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 NCBI Official Full Name    myosin-1
 NCBI Official Synonym Full Names    myosin, heavy chain 1, skeletal muscle, adult
 NCBI Official Symbol    MYH1 [Similar Products]
 NCBI Official Synonym Symbols   
MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D
[Similar Products]
 NCBI Protein Information    myosin-1; myHC-IIx/d; myosin heavy chain 1; myosin heavy chain 2x; myosin heavy chain IIx/d; epididymis luminal protein 71; myosin heavy chain, skeletal muscle, adult 1; myosin, heavy polypeptide 1, skeletal muscle, adult
 UniProt Protein Name    Myosin-1
 UniProt Synonym Protein Names   
Myosin heavy chain 1; Myosin heavy chain 2x; MyHC-2x; Myosin heavy chain IIx/d; MyHC-IIx/d; Myosin heavy chain, skeletal muscle, adult 1
 UniProt Gene Name    MYH1 [Similar Products]
 UniProt Synonym Gene Names    MyHC-2x; MyHC-IIx/d [Similar Products]
 UniProt Entry Name    MYH1_HUMAN
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 NCBI Summary for MYH1    Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MYH1    MYH1: Muscle contraction.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: muscle myosin complex; A band

Molecular Function: calmodulin binding; motor activity; actin binding; ATP binding

Biological Process: muscle contraction; metabolic process
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 Research Articles on MYH1    1. adaptive response to reduced masticatory load was lower numerical and area proportion of MyHC-1 expressing fibres and higher numerical proportion of hybrid fibres in edentulous compared with dentate subjects
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with MYH1 elisa kitPathways associated with MYH1 elisa kit
 Reference Product  PubMed Publications
 MYH7 elisa kit  >11 publications with MYH1 and MYH7
 MYH2 elisa kit  >10 publications with MYH1 and MYH2
 MYH8 elisa kit  >8 publications with MYH1 and MYH8
 MYH6 elisa kit  >4 publications with MYH1 and MYH6
 MYLPF elisa kit  >1 publications with MYH1 and MYLPF
 MYH13 elisa kit  >1 publications with MYH1 and MYH13
 ACTB elisa kit  >1 publications with MYH1 and ACTB
 Products by Pathway  Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
 Translocation Of GLUT4 To The Plasma Membrane Pathway antibodies  Translocation Of GLUT4 To The Plasma Membrane Pathway Diagram
Diseases associated with MYH1 elisa kitOrgans/Tissues associated with MYH1 elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with MYH1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >2 publications with MYH1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with MYH1 and Cardiovascular Diseases
 Chromosome Aberrations Antibodies  >1 publications with MYH1 and Chromosome Aberrations
 Fibrosis Antibodies  >1 publications with MYH1 and Fibrosis
 Prostatic Neoplasms Antibodies  >1 publications with MYH1 and Prostatic Neoplasms
 Heart Diseases Antibodies  >1 publications with MYH1 and Heart Diseases
 Inflammation Antibodies  >1 publications with MYH1 and Inflammation
 Necrosis Antibodies  >1 publications with MYH1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >31 publications with MYH1 and Muscle
 Bone Antibodies  >3 publications with MYH1 and Bone
 Testis Antibodies  >2 publications with MYH1 and Testis
 Heart Antibodies  >2 publications with MYH1 and Heart
 Mouth Antibodies  >1 publications with MYH1 and Mouth
 Uncharacterized Tissue Antibodies  >1 publications with MYH1 and Uncharacterized Tissue
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