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MME elisa kit :: Mouse Neprilysin/CD10 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS176564
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
1x96 Wells  /  $425 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neprilysin/CD10 (MME), ELISA Kit

 Also Known As   

Mouse Neprilysin/CD10 PicoKine ELISA Kit

 Product Synonym Names    Neprilysin; membrane metallo-endopeptidase; Atriopeptidase; CALLA; CD 10; CD10; CD10 antigen; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase); Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); Membrane metallo endopeptidase; Membrane metallo endopeptidase variant 1; Membrane metallo endopeptidase variant 2; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; NEP_HUMAN; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; SFE; Skin fibroblast elastase
 Product Gene Name   

MME elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Manual Insert    Download PDF Manual View PDF Manual
 3D Structure    ModBase 3D Structure for Q61391
 Species Reactivity    Mouse
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 Specificity    Natural and recombinant mouse CD10
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 Samples    Cell culture supernates, serum and plasma (heparin, EDTA)
 Assay Type    Sandwich
 Detection Range    93.7pg/ml-6000pg/ml
 Sensitivity    < 10pg/ml
 Intra-assay Precision    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
 Inter-assay Precision    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
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 Preparation and Storage    Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of MME elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for MME purchase    MBS176564 is a ready-to-use microwell, strip-or-full plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neprilysin/CD10 (MME) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 93.7pg/ml-6000pg/ml in biological research samples containing MME, with an estimated sensitivity of < 10pg/ml. The ELISA analytical biochemical technique of the MBS176564 kit is based on MME antibody-MME antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MME antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MME. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions such as Cell culture supernates, serum and plasma (heparin, EDTA). Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for MME elisa kit

   Principle of the assay: mouse CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD10 has been precoated onto 96-well plates. Standards(sf21, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD10 amount of sample captured in plate.
Background: Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
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 Typical Testing Data/Standard Curve (for reference only) of MME elisa kit    MME elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS176564
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NCBI/Uniprot data below describe general gene information for MME. It may not necessarily be applicable to this product.
 NCBI GI #    51338732
 NCBI GeneID    17380
 NCBI Accession #    Q61391.3 [Other Products]
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 UniProt Primary Accession #    Q61391 [Other Products]
 UniProt Secondary Accession #    Q6NXX5; Q8K251 [Other Products]
 UniProt Related Accession #    Q61391 [Other Products]
 Molecular Weight    85,702 Da
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 NCBI Official Full Name    Neprilysin
 NCBI Official Synonym Full Names    membrane metallo endopeptidase
 NCBI Official Symbol    Mme [Similar Products]
 NCBI Official Synonym Symbols   
NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
[Similar Products]
 NCBI Protein Information    neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; common acute lymphoblastic leukemia antigen
 UniProt Protein Name    Neprilysin
 UniProt Synonym Protein Names   
Atriopeptidase; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD_antigen: CD10
 Protein Family    Neprilysin
 UniProt Gene Name    Mme [Similar Products]
 UniProt Synonym Gene Names    NEP; Neutral endopeptidase; SFE [Similar Products]
 UniProt Entry Name    NEP_MOUSE
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 UniProt Comments for MME    CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.11

Cellular Component: synaptic vesicle; focal adhesion; membrane; axon; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: peptide metabolic process; beta-amyloid metabolic process; creatinine metabolic process; sensory perception of pain; proteolysis
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 Research Articles on MME    1. Data indicate that 5-aza-deoxycytidine induced the demethylation of neprilysin (NEP) gene and significantly increased NEP expression in a dose-dependent manner.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Mouse Neprilysin/CD10 PicoKine ELISA Kit
 Catalog #  Product Name  Catalog #  Product Name
 MBS844735  Human CellExp Neprilysin / CD10, human recombinant  MBS176564  Mouse Neprilysin/CD10 PicoKine ELISA Kit
Pathways associated with MME elisa kitDiseases associated with MME elisa kit
 Products by Pathway  Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Protein Digestion And Absorption Pathway antibodies  Protein Digestion And Absorption Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >22 publications with MME and Nervous System Diseases
 Carcinoma Antibodies  >17 publications with MME and Carcinoma
 Heart Diseases Antibodies  >16 publications with MME and Heart Diseases
 Kidney Diseases Antibodies  >14 publications with MME and Kidney Diseases
 Necrosis Antibodies  >13 publications with MME and Necrosis
 Inflammation Antibodies  >11 publications with MME and Inflammation
 Neoplasms, Experimental Antibodies  >11 publications with MME and Neoplasms, Experimental
 Lung Diseases Antibodies  >10 publications with MME and Lung Diseases
 Disease Models, Animal Antibodies  >10 publications with MME and Disease Models, Animal
 Edema Antibodies  >7 publications with MME and Edema
Organs/Tissues associated with MME elisa kit
 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >71 publications with MME and Other
 Brain Antibodies  >23 publications with MME and Brain
 Liver Antibodies  >21 publications with MME and Liver
 Lung Antibodies  >18 publications with MME and Lung
 Kidney Antibodies  >16 publications with MME and Kidney
 Eye Antibodies  >9 publications with MME and Eye
 Skin Antibodies  >9 publications with MME and Skin
 Prostate Antibodies  >6 publications with MME and Prostate
 Ovary Antibodies  >3 publications with MME and Ovary
 Thyroid Antibodies  >3 publications with MME and Thyroid
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