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Ntn1 elisa kit :: Mouse Netrin-1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS733806
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Netrin-1 (Ntn1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Netrin-1 ELISA Kit

 Product Gene Name   

Ntn1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    601614
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 Species Reactivity    Mouse
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 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    50-1000pg/mL
 Sensitivity    1.0pg/mL
 Intended Uses    This NTn-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NTn-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
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 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Ntn1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Ntn1 purchase    MBS733806 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Netrin-1 (Ntn1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 50-1000pg/mL in biological research samples containing Ntn1, with an estimated sensitivity of 1.0pg/mL. The ELISA analytical biochemical technique of the MBS733806 kit is based on Ntn1 antibody-Ntn1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ntn1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ntn1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for Ntn1 elisa kit

   Intended Uses: This NTn-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse NTn-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: NTn-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NTn-1 antibody and an NTn-1-HRP conjugate. The assay sample and buffer are incubated together with NTn-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTn-1 concentration since NTn-1 from samples and NTn-1-HRP conjugate compete for the anti-NTn-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NTn-1 from the sample, fewer sites are left to bind NTn-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTn-1 concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Ntn1 elisa kit    Neurobiology
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 Typical Testing Data/Standard Curve (for reference only) of Ntn1 elisa kit    Ntn1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS733806. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Ntn1. It may not necessarily be applicable to this product.
 NCBI GI #    148613884
 NCBI GeneID    9423
 NCBI Accession #    NP_004813.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_004822.2 [Other Products]
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 UniProt Secondary Accession #    E9KL51 [Other Products]
 UniProt Related Accession #    O95631 [Other Products]
 Molecular Weight    67,748 Da
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 NCBI Official Full Name    netrin-1
 NCBI Official Synonym Full Names    netrin 1
 NCBI Official Symbol    NTN1 [Similar Products]
 NCBI Official Synonym Symbols   
NTN1L
[Similar Products]
 NCBI Protein Information    netrin-1; netrin 1, mouse, homolog of; epididymis tissue protein Li 131P
 UniProt Protein Name    Netrin-1
 UniProt Synonym Protein Names   
Epididymis tissue protein Li 131P
 Protein Family    Netrin
 UniProt Gene Name    NTN1 [Similar Products]
 UniProt Synonym Gene Names    NTN1L [Similar Products]
 UniProt Entry Name    NET1_HUMAN
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 NCBI Summary for Ntn1    Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Ntn1    NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; extracellular region; basement membrane

Molecular Function: protein binding

Biological Process: axon guidance; inner ear morphogenesis; cell-cell adhesion; establishment of nucleus localization; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance
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 Research Articles on Ntn1    1. Data indicate that dysregulation of expression of netrin-1 NTN1 in smooth muscle cell (SMC) and its chemorepulsive receptor UNC5B in macrophages are involved in the development of atherosclerosis and unstable plaques.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with Ntn1 elisa kitPathways associated with Ntn1 elisa kit
 Reference Product  PubMed Publications
 DCC elisa kit  >6 publications with Ntn1 and DCC
 UNC5B elisa kit  >4 publications with Ntn1 and UNC5B
 UNC5C elisa kit  >2 publications with Ntn1 and UNC5C
 UNC5A elisa kit  >1 publications with Ntn1 and UNC5A
 NEO1 elisa kit  >1 publications with Ntn1 and NEO1
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 DCC Mediated Attractive Signaling Pathway antibodies  DCC Mediated Attractive Signaling Pathway Diagram
 DSCAM Interactions Pathway antibodies  DSCAM Interactions Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Netrin Mediated Repulsion Signals Pathway antibodies  Netrin Mediated Repulsion Signals Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
Diseases associated with Ntn1 elisa kitOrgans/Tissues associated with Ntn1 elisa kit
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with Ntn1 and Inflammation
 Atherosclerosis Antibodies  >2 publications with Ntn1 and Atherosclerosis
 Carcinoma, Hepatocellular Antibodies  >2 publications with Ntn1 and Carcinoma, Hepatocellular
 Disease Models, Animal Antibodies  >2 publications with Ntn1 and Disease Models, Animal
 Pneumonia Antibodies  >1 publications with Ntn1 and Pneumonia
 Nervous System Diseases Antibodies  >1 publications with Ntn1 and Nervous System Diseases
 Prenatal Exposure Delayed Effects Antibodies  >1 publications with Ntn1 and Prenatal Exposure Delayed Effects
 Congenital Abnormalities Antibodies  >1 publications with Ntn1 and Congenital Abnormalities
 Liver Neoplasms Antibodies  >1 publications with Ntn1 and Liver Neoplasms
 Lung Neoplasms Antibodies  >1 publications with Ntn1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with Ntn1 and Brain
 Muscle Antibodies  >3 publications with Ntn1 and Muscle
 Embryonic Tissue Antibodies  >3 publications with Ntn1 and Embryonic Tissue
 Lung Antibodies  >2 publications with Ntn1 and Lung
 Eye Antibodies  >2 publications with Ntn1 and Eye
 Connective Tissue Antibodies  >1 publications with Ntn1 and Connective Tissue
 Ovary Antibodies  >1 publications with Ntn1 and Ovary
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