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NPC1 elisa kit :: Mouse Niemann-Pick disease, type C1 ELISA Kit

Scan QR to view Datasheet Catalog #    MBS906920
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells (LIMIT 1)  /  $250 +1 FREE 8GB USB
48-Strip-Wells  /  $495 +1 FREE 8GB USB
96-Strip-Wells  /  $710 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Niemann-Pick disease, type C1 (NPC1), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Niemann-Pick C1 protein, NPC1 ELISA Kit

 Product Synonym Names    Mouse Niemann-Pick C1 protein (NPC1) ELISA kit; FLJ98532; NPC; ; Niemann-Pick disease; type C1
 Product Gene Name   

NPC1 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS906920 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35604
 Species Reactivity    Mouse
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 Specificity    This assay has high sensitivity and excellent specificity for detection of Mouse NPC1. No significant cross-reactivity or interference between Mouse NPC1 and analogues was observed.
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 Samples    Serum, plasma, tissue homogenates, Cell lysates
 Assay Type    Sandwich
 Detection Range    31.25 pg/ml-2000 pg/ml
 Sensitivity    7.81 pg/ml
 Intra-assay Precision    Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
 Detection Wavelength    450 nm
 Sample Volume    50-100ul
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 Preparation and Storage    Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NPC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NPC1 purchase    MBS906920 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Niemann-Pick disease, type C1 (NPC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 31.25 pg/ml-2000 pg/ml in biological research samples containing NPC1, with an estimated sensitivity of 7.81 pg/ml. The ELISA analytical biochemical technique of the MBS906920 kit is based on NPC1 antibody-NPC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, tissue homogenates, Cell lysates. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Related Product Information for NPC1 elisa kit

   Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NPC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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 Typical Testing Data/Standard Curve (for reference only) of NPC1 elisa kit    NPC1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS906920. Click to request current manual
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NCBI/Uniprot data below describe general gene information for NPC1. It may not necessarily be applicable to this product.
 NCBI GI #    89242146
 NCBI GeneID    18145
 NCBI Accession #    NP_032746.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_008720.2 [Other Products]
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 UniProt Primary Accession #    O35604 [Other Products]
 UniProt Secondary Accession #    O35605; G3X8W9 [Other Products]
 UniProt Related Accession #    O35604 [Other Products]
 Molecular Weight    142,885 Da
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 NCBI Official Full Name    Niemann-Pick C1 protein
 NCBI Official Synonym Full Names    Niemann-Pick type C1
 NCBI Official Symbol    Npc1 [Similar Products]
 NCBI Official Synonym Symbols   
spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
[Similar Products]
 NCBI Protein Information    Niemann-Pick C1 protein; sphingomyelinosis; Niemann Pick type C1
 UniProt Protein Name    Niemann-Pick C1 protein
 Protein Family    Niemann-Pick C1 protein
 UniProt Gene Name    Npc1 [Similar Products]
 UniProt Entry Name    NPC1_MOUSE
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 UniProt Comments for NPC1    NPC1: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPC1). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected. Belongs to the patched family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; lysosomal membrane; endoplasmic reticulum; lysosome; integral to plasma membrane; integral to membrane; extracellular region; nuclear envelope; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; endosome; vesicle

Molecular Function: protein binding; hedgehog receptor activity; cholesterol binding

Biological Process: response to drug; steroid metabolic process; cholesterol metabolic process; cholesterol transport; protein amino acid glycosylation; endocytosis; cholesterol efflux; adult walking behavior; negative regulation of macroautophagy; cholesterol homeostasis; response to cadmium ion; autophagy; lipid metabolic process; lipid raft organization and biogenesis
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 Research Articles on NPC1    1. NPC1(-/-) animals present myelin-like lysosomal deposits in virtually all types of cells of the peripheral and central olfactory system.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with NPC1 elisa kitDiseases associated with NPC1 elisa kit
 Products by Pathway  Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >379 publications with NPC1 and Nervous System Diseases
 Niemann-Pick Disease, Type C Antibodies  >114 publications with NPC1 and Niemann-Pick Disease, Type C
 Disease Models, Animal Antibodies  >86 publications with NPC1 and Disease Models, Animal
 Neurodegenerative Diseases Antibodies  >78 publications with NPC1 and Neurodegenerative Diseases
 Liver Diseases Antibodies  >47 publications with NPC1 and Liver Diseases
 Nerve Degeneration Antibodies  >30 publications with NPC1 and Nerve Degeneration
 Cardiovascular Diseases Antibodies  >24 publications with NPC1 and Cardiovascular Diseases
 Mental Disorders Antibodies  >23 publications with NPC1 and Mental Disorders
 Atherosclerosis Antibodies  >23 publications with NPC1 and Atherosclerosis
 Vascular Diseases Antibodies  >18 publications with NPC1 and Vascular Diseases
Organs/Tissues associated with NPC1 elisa kit
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >149 publications with NPC1 and Brain
 Liver Antibodies  >100 publications with NPC1 and Liver
 Blood Antibodies  >52 publications with NPC1 and Blood
 Ovary Antibodies  >36 publications with NPC1 and Ovary
 Skin Antibodies  >20 publications with NPC1 and Skin
 Spleen Antibodies  >17 publications with NPC1 and Spleen
 Lung Antibodies  >14 publications with NPC1 and Lung
 Bone Antibodies  >10 publications with NPC1 and Bone
 Bone Marrow Antibodies  >9 publications with NPC1 and Bone Marrow
 Kidney Antibodies  >8 publications with NPC1 and Kidney
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