Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow ELISA Kit rightarrow NOG rightarrow Mouse NOG  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • ELA4 ELISA Kit
 • CALB1 Blocking Peptide
 • Probable alpha,alpha-trehalose-phosp hate synthase [UDP-formi... Recombinant Protein
 • ATF4 Antibody
 • Phosphocreatine (PCr) Antibody
 • YME1-like 1 (YME1L1) Antibody
 • Triglyceride (TG) ELISA Kit
 • growth differentiation factor 5 (GDF5) ELISA Kit
 • Vesicular Inhibitory Amino Acid Transporter (VIAAT) ELISA Kit
 • Goat Anti-Rabbit IgM+IgG (H+L chain specific) Secondary Antibody
 • Mac-2 Binding Protein Glycosylation Isomer (M2BPGI) ELISA Kit
 • G Protein Coupled Estrogen Receptor 1 (GPER) ELISA Kit
 • O-acetyl transferase (OAC) Recombinant Protein
 • Centrosomal protein of 135 kDa (CEP135) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

NOG elisa kit :: Mouse Noggin (NOG) ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2021387 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
24-Strip-Wells  /  $270 +1 FREE 8GB USB
48-Strip-Wells  /  $505 +1 FREE 8GB USB
96-Strip-Wells  /  $685 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Noggin (NOG), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Noggin (NOG) ELISA Kit

 Product Gene Name   

NOG elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 Chromosome Location    Chromosome: 17; NC_000017.11 (56593699..56595590). Location: 17q22
 OMIM    184460
 3D Structure    ModBase 3D Structure for Q13253
Section Bar
 Species Reactivity    Mouse
Section Bar
 Specificity    This assay has high sensitivity and excellent specificity for detection of Noggin (NOG).
No significant cross-reactivity or interference between Noggin (NOG) and analogues was observed.
Section Bar
 Assay Type    Double-antibody Sandwich
 Samples    Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
 Detection Range    0.156-10ng/mL
 Sensitivity    < 0.066ng/mL
 Application    Enzyme-linked immunosorbent assay for Antigen Detection.
 Intra-assay Precision (Precision within an assay)    3 samples with low, middle and high level Noggin (NOG) were tested 20 times on one plate, respectively.
 Inter-assay Precision (Precision between assays)    3 samples with low, middle and high level Noggin (NOG) were tested on 3 different plates, 8 replicates in each plate.
 CV(%) =    SD/meanX100
 Intra-Assay    CV<10%
 Inter-Assay    CV<12%
Section Bar
 Preparation and Storage    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of NOG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for NOG purchase    MBS2021387 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Noggin (NOG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 0.156-10ng/mL in biological research samples containing NOG, with an estimated sensitivity of < 0.066ng/mL. The ELISA analytical biochemical technique of the MBS2021387 kit is based on NOG antibody-NOG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar
 

Related Product Information for NOG elisa kit

   The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Noggin (NOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Noggin (NOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Noggin (NOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noggin (NOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
 Product Categories/Family for NOG elisa kit    Signal transduction; Immune molecule; Neuroscience; Bone metabolism
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of NOG elisa kit    NOG elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS2021387. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for NOG. It may not necessarily be applicable to this product.
 NCBI GI #    4885523
 NCBI GeneID    9241
 NCBI Accession #    NP_005441.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005450.4 [Other Products]
Section Bar
 UniProt Primary Accession #    Q13253 [Other Products]
 UniProt Related Accession #    Q13253 [Other Products]
 Molecular Weight    25,774 Da
Table BarTOPTable Bar
 NCBI Official Full Name    noggin
 NCBI Official Synonym Full Names    noggin
 NCBI Official Symbol    NOG [Similar Products]
 NCBI Official Synonym Symbols   
SYM1; SYNS1
[Similar Products]
 NCBI Protein Information    noggin; symphalangism 1 (proximal)
 UniProt Protein Name    Noggin
 Protein Family    Noggin
 UniProt Gene Name    NOG [Similar Products]
 UniProt Entry Name    NOGG_HUMAN
Section Bar
 NCBI Summary for NOG    The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for NOG    NOG: Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in NOG are a cause of symphalangism proximal syndrome (SYM1). SYM1 is characterized by the hereditary absence of the proximal interphalangeal (PIP) joints (Cushing symphalangism). Severity of PIP joint involvement diminishes towards the radial side. Distal interphalangeal joints are less frequently involved and metacarpophalangeal joints are rarely affected whereas carpal bone malformation and fusion are common. In the lower extremities, tarsal bone coalition is common. Conducive hearing loss is seen and is due to fusion of the stapes to the petrous part of the temporal bone. Defects in NOG are the cause of multiple synostoses syndrome type 1 (SYNS1); also known as synostoses, multiple, with brachydactyly/symphalangism-brachydactyly syndrome. SYNS1 is characterized by tubular-shaped (hemicylindrical) nose with lack of alar flare, otosclerotic deafness, and multiple progressive joint fusions commencing in the hand. The joint fusions are progressive, commencing in the fifth proximal interphalangeal joint in early childhood (or at birth in some individuals) and progressing in an ulnar-to-radial and proximal- to-distal direction. With increasing age, ankylosis of other joints, including the cervical vertebrae, hips, and humeroradial joints, develop. Defects in NOG are the cause of tarsal-carpal coalition syndrome (TCC). TCC is an autosomal dominant disorder characterized by fusion of the carpals, tarsals and phalanges, short first metacarpals causing brachydactyly, and humeroradial fusion. TCC is allelic to SYM1, and different mutations in NOG can result in either TCC or SYM1 in different families. Defects in NOG are a cause of stapes ankylosis with broad thumb and toes (SABTS); also known as Teunissen- Cremers syndrome. SABTS is a congenital autosomal dominant disorder that includes hyperopia, a hemicylindrical nose, broad thumbs, great toes, and other minor skeletal anomalies but lacked carpal and tarsal fusion and symphalangism. Defects in NOG are the cause of brachydactyly type B2 (BDB2). BDB2 is a subtype of brachydactyly characterized by hypoplasia/aplasia of distal phalanges in combination with distal symphalangism, fusion of carpal/tarsal bones, and partial cutaneous syndactyly. Belongs to the noggin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; protein homodimerization activity; cytokine binding

Biological Process: limb development; wound healing; somatic stem cell maintenance; embryonic skeletal development; motor axon guidance; negative regulation of transcription from RNA polymerase II promoter; middle ear morphogenesis; negative regulation of BMP signaling pathway; BMP signaling pathway; notochord morphogenesis; cell differentiation in hindbrain; negative regulation of cardiac muscle cell proliferation; axial mesoderm development; negative regulation of osteoblast differentiation; negative regulation of cell migration; skeletal development; nervous system development; neural plate morphogenesis; in utero embryonic development; osteoblast differentiation; mesoderm formation; dorsal/ventral pattern formation; endoderm formation; pituitary gland development; spinal cord development; cartilage development; negative regulation of astrocyte differentiation; neural tube closure; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; positive regulation of epithelial cell proliferation

Disease: Stapes Ankylosis With Broad Thumb And Toes; Symphalangism, Proximal, 1a; Brachydactyly, Type B2; Tarsal-carpal Coalition Syndrome; Multiple Synostoses Syndrome 1
Table BarTOPTable Bar
 Research Articles on NOG    1. Even though gremlin 1 and noggin were not widely expressed in adult tissues, in a subset of organs their expression pattern indicated a potential role in normal tissue homeostasis as well as in malignancies.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with NOG elisa kitPathways associated with NOG elisa kit
 Reference Product  PubMed Publications
 BMP4 elisa kit  >18 publications with NOG and BMP4
 GDF5 elisa kit  >11 publications with NOG and GDF5
 BMP2 elisa kit  >9 publications with NOG and BMP2
 BMPR1A elisa kit  >8 publications with NOG and BMPR1A
 BMPR1B elisa kit  >4 publications with NOG and BMPR1B
 BMPR2 elisa kit  >3 publications with NOG and BMPR2
 BMP7 elisa kit  >3 publications with NOG and BMP7
 BMP6 elisa kit  >2 publications with NOG and BMP6
 Products by Pathway  Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By BMP Pathway antibodies  Signaling By BMP Pathway Diagram
 TGF Beta Signaling Pathway antibodies  TGF Beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
Diseases associated with NOG elisa kitOrgans/Tissues associated with NOG elisa kit
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >71 publications with NOG and Neoplasms
 Disease Models, Animal Antibodies  >53 publications with NOG and Disease Models, Animal
 Nervous System Diseases Antibodies  >33 publications with NOG and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >23 publications with NOG and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >12 publications with NOG and Neoplasm Metastasis
 Inflammation Antibodies  >11 publications with NOG and Inflammation
 Liver Diseases Antibodies  >11 publications with NOG and Liver Diseases
 Liver Neoplasms Antibodies  >10 publications with NOG and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >9 publications with NOG and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >8 publications with NOG and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >109 publications with NOG and Blood
 Spleen Antibodies  >22 publications with NOG and Spleen
 Brain Antibodies  >15 publications with NOG and Brain
 Uterus Antibodies  >8 publications with NOG and Uterus
 Prostate Antibodies  >3 publications with NOG and Prostate
 Placenta Antibodies  >1 publications with NOG and Placenta
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions