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Rnase2 elisa kit :: Mouse Non-secretory ribonuclease ELISA Kit

Scan QR to view Datasheet Catalog #    MBS2883405
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $380 +1 FREE 8GB USB
96-Strip-Wells  /  $500 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,170 +2 FREE 8GB USB
10x96-Strip-Wells  /  $3,840 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Non-secretory ribonuclease (Rnase2), ELISA Kit

★Popular Item★
 Also Known As   

Mouse Non-secretory ribonuclease ELISA Kit

 Product Synonym Names    Non-secretory ribonuclease; Eosinophil cationic-type ribonuclease 4; MR-4; Ribonuclease 2; RNase 2; Rnase2; Ear4; 3.1.27.5
 Product Gene Name   

Rnase2 elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 MBS2883405 Testing Data    Testing Data PDF
 3D Structure    ModBase 3D Structure for O35291
 Species Reactivity    Mouse
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 Assay Type    Sandwich
 Detection Range    78-5000 pg/mL
 Sensitivity    39.4 pg/mL
 Intra-Assay CV    <=6.1%
 Inter-Assay CV    <=8.6%
 Recovery    97%
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 Preparation and Storage    For long term storage, please store the entire kit at -20 degree C.
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Rnase2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Rnase2 purchase    MBS2883405 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Non-secretory ribonuclease (Rnase2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 78-5000 pg/mL in biological research samples containing Rnase2, with an estimated sensitivity of 39.4 pg/mL. The ELISA analytical biochemical technique of the MBS2883405 kit is based on Rnase2 antibody-Rnase2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Rnase2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Rnase2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Product Description specifically for Rnase2 elisa kit

   Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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 Typical Testing Data/Standard Curve (for reference only) of Rnase2 elisa kit    Rnase2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
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Sample Manual Insert of MBS2883405. Click to request current manual
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NCBI/Uniprot data below describe general gene information for Rnase2. It may not necessarily be applicable to this product.
 NCBI GI #    62945404
 NCBI GeneID    53877
 NCBI Accession #    NP_001017422.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001017422.1 [Other Products]
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 UniProt Primary Accession #    O35291 [Other Products]
 UniProt Related Accession #    O35291 [Other Products]
 Molecular Weight    17,723 Da
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 NCBI Official Full Name    non-secretory ribonuclease
 NCBI Official Synonym Full Names    eosinophil-associated, ribonuclease A family, member 4
 NCBI Official Symbol    Ear4 [Similar Products]
 NCBI Official Synonym Symbols   
mR4; RNS2; Rnase2
[Similar Products]
 NCBI Protein Information    non-secretory ribonuclease
 UniProt Protein Name    Non-secretory ribonuclease
 UniProt Synonym Protein Names   
Eosinophil cationic-type ribonuclease 4; MR-4; Ribonuclease 2; RNase 2
 Protein Family    Non-secretory ribonuclease
 UniProt Gene Name    Rnase2 [Similar Products]
 UniProt Synonym Gene Names    Ear4; RNase 2 [Similar Products]
 UniProt Entry Name    RNAS2_MOUSE
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 UniProt Comments for Rnase2    RNASE2: This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities. Belongs to the pancreatic ribonuclease family.

Protein type: RNA processing; Motility/polarity/chemotaxis; Ribonuclease; EC 3.1.27.5

Cellular Component: lysosome

Molecular Function: endonuclease activity; hydrolase activity; nuclease activity; nucleic acid binding; pancreatic ribonuclease activity; ribonuclease activity
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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