Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource rightarrow ELISA Kit rightarrow Orexin rightarrow Mouse Orexin  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
Our Best Sellers moreseparator
 • potassium inwardly-rectifying channel, subfamily J, member 1... Antibody
 • Malondialdehyde (MDA ) ELISA Kit
 • Legionella antibody lgM (LP Ab-lgM) ELISA Kit
 • pH2AX Antibody
 • IgG (H&L) Secondary Antibody
 • Interleukin 34 (IL-34) ELISA Kit
 • Cathepsin V (CTSV) Antibody
 • Ycf54-like protein (slr1780) Recombinant Protein
 • solute carrier family 22 (organic anion transporter) , membe... ELISA Kit
 • S100A4 - FSP1 Antibody
 • Interleukin-26 (IL26) ELISA Kit
 • CLEC3A Antibody
 • Ornithine Carbamoyl Transferase (OCT) ELISA Kit
 downarrow more ...
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

Orexin elisa kit :: Mouse Orexin A ELISA Kit

Scan QR to view Datasheet Catalog #    MBS722375
Typical Testing Data/Standard Curve (for reference only)
Unit / Price
48-Strip-Wells  /  $440 +1 FREE 8GB USB
96-Strip-Wells  /  $640 +1 FREE 8GB USB
5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Orexin A, ELISA Kit

 Also Known As   

Mouse Orexin A ELISA Kit

 Product Gene Name   

Orexin elisa kit

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
  Sample Manual Insert    Download PDF Manual View PDF Manual
 Request for Current Manual Insert    Request Current Manual
 OMIM    602358
Section Bar
 Species Reactivity    Mouse
Section Bar
Section Bar
 Samples    Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
 Assay Type    Competitive
 Detection Range    5.0-100ng/mL
 Sensitivity    1.0ng/mL
 Intended Uses    This OX-A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse OX-A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Section Bar
 Preparation and Storage    Store all reagents at 2-8 degree C.
 Sample Preparation    We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
 Product Note    Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
 Other Notes    Small volumes of Orexin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for Orexin purchase    MBS722375 is a ready-to-use microwell, strip plate Competitive ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Orexin A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 5.0-100ng/mL in biological research samples containing Orexin, with an estimated sensitivity of 1.0ng/mL. The ELISA analytical biochemical technique of the MBS722375 kit is based on Orexin antibody-Orexin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Orexin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Orexin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions such as Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Table BarTOPTable Bar

Related Product Information for Orexin elisa kit

   Principle of the Assay: OX-A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OX-A antibody and an OX-A-HRP conjugate. The assay sample and buffer are incubated together with OX-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OX-A concentration since OX-A from samples and OX-A-HRP conjugate compete for the anti-OX-A antibody binding site. Since the number of sites is limited, as more sites are occupied by OX-A from the sample, fewer sites are left to bind OX-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OX-A concentration in each sample is interpolated from this standard curve.
 Product Categories/Family for Orexin elisa kit    Neurobiology
Section Bar
 Typical Testing Data/Standard Curve (for reference only) of Orexin elisa kit    Orexin elisa kit Typical Testing Data/Standard Curve (for reference only) image
Table BarTOPTable Bar
Sample Manual Insert of MBS722375. Click to request current manual
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for Orexin. It may not necessarily be applicable to this product.
 NCBI GI #    4557635
 NCBI GeneID    3060
 NCBI Accession #    NP_001515.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001524.1 [Other Products]
Section Bar
 UniProt Related Accession #    O43612 [Other Products]
 Molecular Weight    13,363 Da
Table BarTOPTable Bar
 NCBI Official Full Name    orexin
 NCBI Official Synonym Full Names    hypocretin (orexin) neuropeptide precursor
 NCBI Official Symbol    HCRT [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    orexin; prepro-orexin
 UniProt Protein Name    Orexin
 UniProt Synonym Protein Names   
Hypocretin; Hcrt
 UniProt Gene Name    HCRT [Similar Products]
 UniProt Synonym Gene Names    OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2 [Similar Products]
 UniProt Entry Name    OREX_HUMAN
Section Bar
 NCBI Summary for Orexin    This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]
Section Bar
 UniProt Comments for Orexin    HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family.

Protein type: Hormone

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule

Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding

Biological Process: synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; eating behavior; negative regulation of DNA replication; negative regulation of potassium ion transport; protein kinase C activation; regulation of neurotransmitter secretion; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of calcium ion transport; regulation of excitatory postsynaptic membrane potential; positive regulation of transmission of nerve impulse

Disease: Narcolepsy 1
Table BarTOPTable Bar
 Research Articles on Orexin    1. Rapid postoperative increases in plasma Orexin are associated with better improvement of glucose and lipid profiles following bariatric surgery.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with Orexin elisa kitPathways associated with Orexin elisa kit
 Reference Product  PubMed Publications
 HCRTR1 elisa kit  >87 publications with Orexin and HCRTR1
 FOS elisa kit  >26 publications with Orexin and FOS
 TRH elisa kit  >2 publications with Orexin and TRH
 CCK elisa kit  >2 publications with Orexin and CCK
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway antibodies  Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
Diseases associated with Orexin elisa kitOrgans/Tissues associated with Orexin elisa kit
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >72 publications with Orexin and Nervous System Diseases
 Narcolepsy 1 Antibodies  >60 publications with Orexin and Narcolepsy 1
 Disease Models, Animal Antibodies  >12 publications with Orexin and Disease Models, Animal
 Bradycardia Antibodies  >8 publications with Orexin and Bradycardia
 Carcinoma Antibodies  >7 publications with Orexin and Carcinoma
 Cardiovascular Diseases Antibodies  >6 publications with Orexin and Cardiovascular Diseases
 Anxiety Disorders Antibodies  >6 publications with Orexin and Anxiety Disorders
 Pain Antibodies  >5 publications with Orexin and Pain
 Intestinal Diseases Antibodies  >5 publications with Orexin and Intestinal Diseases
 Weight Loss Antibodies  >4 publications with Orexin and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >195 publications with Orexin and Brain
 Connective Tissue Antibodies  >1 publications with Orexin and Connective Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions